Restriction map for
pFN21AE6625
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# NO CUT ENZYMES ( 161 enzymes) #
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Acc65I AccIII AciI AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspHI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BcgI
BciVI BclI BcnI BfrI BglI
BlnI BlpI BmyI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsiYI BslI BsmI
Bsp1286I Bsp1407I BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HapII HhaI
HinP1I HincII HindII HindIII HpaI
HpaII HphI KasI KpnI KspI
MamI MfeI MluNI MroI MscI
MspI MunI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Van91I XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 5 GACGTC GACGT^C
AccI 62 GTAGAC GT^[AC][GT]AC
AccII 386 CGCG CG^CG
AcsI 909 AAATTT [GA]^AATT[CT]
AcyI 5 GACGTC G[GA]^CG[CT]C
AflIII 385 ACGCGT A^C[GA][CT]GT
ApoI 909 AAATTT [GA]^AATT[CT]
AseI 957 ATTAAT AT^TAAT
AsnI 957 ATTAAT AT^TAAT
Asp700I 251 GAAGTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 332 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 934 GCTGAGG CCTCAGC(-5/-2)
BpmI 231 CTCCAG CTGGAG(16/14)
BsaHI 5 GACGTC G[GA]^CG[CT]C
BsaI 395 GGTCTC GGTCTC(1/5)
BseRI 72 CTCCTC GAGGAG(10/8)
BsmBI 337 CGTCTC CGTCTC(1/5)
BsmFI 3 GGGAC GGGAC(10/14)
BspDI 203 ATCGAT AT^CGAT
BstUI 386 CGCG CG^CG
Cac8I 197 GCAGGC GC[ACGT]^[ACGT]GC
ClaI 203 ATCGAT AT^CGAT
DdeI 935 CTGAG C^T[ACGT]AG
EarI 219 CTCTTC CTCTTC(1/4)
HgaI 194 GACGC GACGC(5/10)
Hin1I 5 GACGTC G[GA]^CG[CT]C
Ksp632I 219 CTCTTC CTCTTC(1/4)
MaeIII 374 GTCAC ^GT[ACGT]AC
MluI 385 ACGCGT A^CGCGT
MseI 958 TTAA T^TAA
MslI 178 CATTCTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 386 CGCG CG^CG
PaeR7I 75 CTCGAG C^TCGAG
PflFI 332 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 5 GACGTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 957 ATTAAT AT^TAAT
SapI 218 GCTCTTC GCTCTTC(1/4)
ScaI 832 AGTACT AGT^ACT
SmlI 75 CTCGAG C^T[CT][GA]AG
Tru9I 958 TTAA T^TAA
Tsp45I 374 GTCAC ^GT[GC]AC
Tth111I 332 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 554 CCAGACTACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 75 CTCGAG C^TCGAG
XmnI 251 GAAGTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AfaI 833 GTAC GT^AC
879 GTAC
AlwI 823 GATCC GGATC(4/5)
850 GATCC
AlwNI 17 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
815 CAGTTGCTG
AvaI 75 CTCGAG C^[CT]CG[GA]G
398 CTCGGG
BbsI 191 GAAGAC GAAGAC(2/6)
649 GAAGAC
BbvI 577 GCTGC GCAGC(8/12)
580 GCTGC
BfaI 296 CTAG C^TAG
770 CTAG
BglII 145 AGATCT A^GATCT
159 AGATCT
BpuAI 191 GAAGAC GAAGAC(2/6)
649 GAAGAC
BsaJI 662 CCCTGG C^C[ACGT][ACGT]GG
896 CCCTGG
BsmAI 338 GTCTC GTCTC(1/5)
396 GTCTC
BsoBI 75 CTCGAG C^[CT]CG[GA]G
398 CTCGGG
BsrI 371 CCAGT ACTGG(1/-1)
830 CCAGT
BstF5I 861 CATCC GGATG(2/0)
904 CATCC
Fnu4HI 577 GCTGC GC^[ACGT]GC
580 GCTGC
FokI 861 CATCC GGATG(9/13)
904 CATCC
HinfI 496 GAATC G^A[ACGT]TC
712 GATTC
ItaI 577 GCTGC GC^[ACGT]GC
580 GCTGC
MaeI 296 CTAG C^TAG
770 CTAG
MspA1I 536 CAGCTG C[AC]G^C[GT]G
562 CAGCTG
NlaIV 229 GGCTCC GG[ACGT]^[ACGT]CC
765 GGAACC
PvuII 536 CAGCTG CAG^CTG
562 CAGCTG
RsaI 833 GTAC GT^AC
879 GTAC
SfcI 279 CTATAG C^T[GA][CT]AG
559 CTACAG
TfiI 496 GAATC G^A[AT]TC
712 GATTC
TseI 577 GCTGC G^C[AT]GC
580 GCTGC
TspRI 360 CAGTG CA[GC]TG[ACGT][ACGT]^
761 CAGTG
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# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AluI AvaII BssKI BstNI BstYI
Cfr13I DpnII EcoRII HaeIII MaeII
MboI MboII MflI MnlI MvaI
MwoI NdeII Sau3AI Sau96I ScrFI
SfaNI TaqI Tsp509I TthHB8I XhoII