Restriction map for
pFN21AE4381
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AvrII BalI
BamHI BbeI BbrPI BbsI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaHI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrGI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI HaeII
Hin1I HindIII KasI KpnI KspI
MflI MluI MluNI MroI MscI
NaeI NarI NdeI NgoMIV NotI
NruI NsiI NspV PacI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI XbaI XcmI XhoII
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# ONE CUT ENZYMES ( 47 enzymes) #
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AgeI 729 ACCGGT A^CCGGT
AlwNI 535 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 388 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 825 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1166 TGCGCA TGC^GCA
BcgI 363 CGAAGTCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1018 CTAG C^TAG
BsaBI 318 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 830 GGTCTC GGTCTC(1/5)
BseRI 426 GAGGAG GAGGAG(10/8)
BsgI 18 CTGCAC GTGCAG(16/14)
BsmFI 151 GGGAC GGGAC(10/14)
BsrDI 820 GCAATG GCAATG(2/0)
BsrFI 729 ACCGGT [GA]^CCGG[CT]
BssHII 139 GCGCGC G^CGCGC
Cfr10I 729 ACCGGT [GA]^CCGG[CT]
DsaI 596 CCATGG C^C[GA][CT]GG
EarI 36 GAAGAG CTCTTC(1/4)
FspI 1166 TGCGCA TGC^GCA
HaeIII 111 GGCC GG^CC
HgaI 993 GCGTC GACGC(5/10)
HincII 343 GTTAAC GT[CT]^[GA]AC
HindII 343 GTTAAC GT[CT]^[GA]AC
HpaI 343 GTTAAC GTT^AAC
Ksp632I 36 GAAGAG CTCTTC(1/4)
MaeI 1018 CTAG C^TAG
MaeII 154 ACGT A^CGT
MamI 318 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 983 CAATTG C^AATTG
MseI 344 TTAA T^TAA
MspA1I 784 CAGCTG C[AC]G^C[GT]G
MunI 983 CAATTG C^AATTG
NcoI 596 CCATGG C^CATGG
NheI 1017 GCTAGC G^CTAGC
PflFI 825 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 636 CCAAGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 729 ACCGGT A^CCGGT
PstI 541 CTGCAG CTGCA^G
PvuII 784 CAGCTG CAG^CTG
SmaI 107 CCCGGG CCC^GGG
Tru9I 344 TTAA T^TAA
Tsp45I 274 GTGAC ^GT[GC]AC
Tth111I 825 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 636 CCAAGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 107 CCCGGG C^CCGGG
XmaI 107 CCCGGG C^CCGGG
XmnI 388 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 138 CGCG CG^CG
140 CGCG
AcsI 115 AAATTC [GA]^AATT[CT]
290 AAATTT
AfaI 651 GTAC GT^AC
750 GTAC
AflIII 624 ACATGT A^C[GA][CT]GT
1137 ACATGT
AlwI 723 GATCC GGATC(4/5)
1069 GGATC
ApoI 115 AAATTC [GA]^AATT[CT]
290 AAATTT
BbvCI 163 CCTCAGC CCTCAGC(-5/-2)
1012 CCTCAGC
BcnI 107 CCCGG CC^[GC]GG
108 CCGGG
BpmI 532 CTCCAG CTGGAG(16/14)
423 CTGGAG
BsaWI 579 ACCGGA [AT]^CCGG[AT]
729 ACCGGT
BsiEI 125 CGGTCG CG[GA][CT]^CG
577 CGACCG
BsmAI 7 GTCTC GTCTC(1/5)
831 GTCTC
BspLU11I 624 ACATGT A^CATGT
1137 ACATGT
BstUI 138 CGCG CG^CG
140 CGCG
HphI 49 GGTGA GGTGA(8/7)
273 GGTGA
MaeIII 274 GTGAC ^GT[ACGT]AC
587 GTAAC
MvnI 138 CGCG CG^CG
140 CGCG
NciI 107 CCCGG CC^[GC]GG
108 CCGGG
NspI 624 ACATGT [GA]CATG^[CT]
1137 ACATGT
PaeR7I 547 CTCGAG C^TCGAG
663 CTCGAG
RsaI 651 GTAC GT^AC
750 GTAC
SacI 75 GAGCTC GAGCT^C
793 GAGCTC
SmlI 547 CTCGAG C^T[CT][GA]AG
663 CTCGAG
TspRI 899 CACTG CA[GC]TG[ACGT][ACGT]^
913 CAGTG
XhoI 547 CTCGAG C^TCGAG
663 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AluI AspHI AvaI AvaII
BanI BanII BbvI BmyI BsaJI
BsiHKAI BsiYI BslI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII EcoRII
EcoT14I Fnu4HI FokI HapII HhaI
HinP1I HinfI HpaII ItaI MboI
MboII MnlI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI TseI Tsp509I
TthHB8I