Restriction map for
pFN21AE9438
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# NO CUT ENZYMES ( 169 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BcnI BfaI BfrI BglI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1286I BspDI BspEI BspHI BsrBI
BsrDI BsrI BssHII BssSI BstAPI
BstEII BstPI BstUI BstXI Bsu36I
CelII CfoI ClaI CpoI DraI
DraII DraIII DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT22I
FauI FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MaeII MluI MluNI MroI
MscI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 375 GTATAC GT^[AC][GT]AC
AflIII 371 ACATGT A^C[GA][CT]GT
AvaII 313 GGACC G^G[AT]CC
BamHI 927 GGATCC G^GATCC
BclI 500 TGATCA T^GATCA
BsiYI 683 CCATATCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 683 CCATATCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 443 GGGAC GGGAC(10/14)
Bsp1407I 455 TGTACA T^GTACA
BspLU11I 371 ACATGT A^CATGT
BspMI 787 ACCTGC ACCTGC(4/8)
BsrFI 135 GCCGGT [GA]^CCGG[CT]
BsrGI 455 TGTACA T^GTACA
Bst1107I 375 GTATAC GTA^TAC
BstBI 287 TTCGAA TT^CGAA
BstZ17I 375 GTATAC GTA^TAC
Cfr10I 135 GCCGGT [GA]^CCGG[CT]
DdeI 837 CTTAG C^T[ACGT]AG
DrdI 121 GACACAATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 815 GAATTC G^AATTC
FbaI 500 TGATCA T^GATCA
HapII 136 CCGG C^CGG
HpaII 136 CCGG C^CGG
MfeI 125 CAATTG C^AATTG
MseI 584 TTAA T^TAA
MspA1I 633 CAGCTG C[AC]G^C[GT]G
MspI 136 CCGG C^CGG
MunI 125 CAATTG C^AATTG
NlaIV 927 GGATCC GG[ACGT]^[ACGT]CC
NspI 371 ACATGT [GA]CATG^[CT]
NspV 287 TTCGAA TT^CGAA
PstI 339 CTGCAG CTGCA^G
PvuII 633 CAGCTG CAG^CTG
SfcI 339 CTGCAG C^T[GA][CT]AG
SfuI 287 TTCGAA TT^CGAA
SspBI 455 TGTACA T^GTACA
TaqI 288 TCGA T^CGA
Tru9I 584 TTAA T^TAA
TthHB8I 288 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 115 GAATTT [GA]^AATT[CT]
815 GAATTC
ApoI 115 GAATTT [GA]^AATT[CT]
815 GAATTC
BbvI 42 GCTGC GCAGC(8/12)
635 GCTGC
BsaBI 412 GATCGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
660 GATTCTGATC
BsaJI 316 CCTTGG C^C[ACGT][ACGT]GG
393 CCTTGG
BstYI 194 GGATCT [GA]^GATC[CT]
927 GGATCC
Cfr13I 85 GGGCC G^G[ACGT]CC
313 GGACC
EaeI 48 CGGCCA [CT]^GGCC[GA]
647 TGGCCG
EcoT14I 316 CCTTGG C^C[AT][AT]GG
393 CCTTGG
Fnu4HI 42 GCTGC GC^[ACGT]GC
635 GCTGC
HgaI 15 GACGC GACGC(5/10)
270 GACGC
ItaI 42 GCTGC GC^[ACGT]GC
635 GCTGC
MamI 412 GATCGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
660 GATTCTGATC
MflI 194 GGATCT [GA]^GATC[CT]
927 GGATCC
MnlI 261 CCTC CCTC(7/6)
556 GAGG
MslI 621 CACCTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1065 CACTGATGTG
MwoI 71 GCAACAGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
407 GCATTGATCGC
PleI 445 GACTC GAGTC(4/5)
57 GAGTC
Sau96I 85 GGGCC G^G[ACGT]CC
313 GGACC
StyI 316 CCTTGG C^C[AT][AT]GG
393 CCTTGG
TfiI 660 GATTC G^A[AT]TC
856 GAATC
TseI 42 GCTGC G^C[AT]GC
635 GCTGC
XhoII 194 GGATCT [GA]^GATC[CT]
927 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AfaI AluI AlwI BsgI BssKI
BstF5I BstNI Cac8I DpnII EcoRII
FokI HaeIII HinfI HphI MaeIII
MboI MboII MvaI NdeII NlaIII
RsaI Sau3AI ScrFI SfaNI Tsp45I
Tsp509I TspRI