Restriction map for
pFN21AB8949
##################################
# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AlwI
AocI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AviII AvrII BamHI BbrPI BbvCI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BseAI BseRI BsiHKAI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspMI BsrBI BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstXI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DdeI DraI
DrdI Eam1105I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FokI
FspI HindIII HpaI KpnI MamI
MfeI MflI MluI MroI MunI
NcoI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SbfI ScaI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp45I Tsp509I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccI 646 GTCGAC GT^[AC][GT]AC
AfaI 714 GTAC GT^AC
AflIII 961 ACATGT A^C[GA][CT]GT
Aor51HI 28 AGCGCT AGC^GCT
AvaI 165 CTCGGG C^[CT]CG[GA]G
AvaII 536 GGTCC G^G[AT]CC
BalI 675 TGGCCA TGG^CCA
BbeI 346 GGCGCC GGCGC^C
BcgI 774 CGAGAACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 616 CTAG C^TAG
BsaWI 240 TCCGGT [AT]^CCGG[AT]
BsiEI 98 CGGCCG CG[GA][CT]^CG
BsoBI 165 CTCGGG C^[CT]CG[GA]G
BspLU11I 961 ACATGT A^CATGT
BssSI 214 CACGAG CACGAG(-5/-1)
DpnII 162 GATC ^GATC
DraIII 292 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 98 CGGCCG C^GGCCG
EarI 229 CTCTTC CTCTTC(1/4)
EclXI 98 CGGCCG C^GGCCG
Eco47III 28 AGCGCT AGC^GCT
Eco52I 98 CGGCCG C^GGCCG
EcoT14I 936 CCTTGG C^C[AT][AT]GG
FseI 189 GGCCGGCC GGCCGG^CC
HgaI 354 GACGC GACGC(5/10)
HincII 646 GTCGAC GT[CT]^[GA]AC
HindII 646 GTCGAC GT[CT]^[GA]AC
KasI 346 GGCGCC G^GCGCC
Ksp632I 229 CTCTTC CTCTTC(1/4)
MaeI 616 CTAG C^TAG
MaeII 337 ACGT A^CGT
MboI 162 GATC ^GATC
MluNI 675 TGGCCA TGG^CCA
MscI 675 TGGCCA TGG^CCA
MseI 629 TTAA T^TAA
NaeI 190 GCCGGC GCC^GGC
NarI 346 GGCGCC GG^CGCC
NdeI 477 CATATG CA^TATG
NdeII 162 GATC ^GATC
NgoMIV 190 GCCGGC G^CCGGC
NspI 961 ACATGT [GA]CATG^[CT]
PflMI 505 CCATGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 535 GGGTCCC [GA]G^G[AT]CC[CT]
RsaI 714 GTAC GT^AC
SalI 646 GTCGAC G^TCGAC
SapI 228 GCTCTTC GCTCTTC(1/4)
Sau3AI 162 GATC ^GATC
SexAI 293 ACCTGGT A^CC[AT]GGT
SfoI 346 GGCGCC GGC^GCC
StyI 936 CCTTGG C^C[AT][AT]GG
Tru9I 629 TTAA T^TAA
TspRI 277 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 505 CCATGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 138 CGCG CG^CG
150 CGCG
AcyI 346 GGCGCC G[GA]^CG[CT]C
354 GACGCC
AlwNI 638 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
879 CAGCGACTG
ApaI 123 GGGCCC GGGCC^C
168 GGGCCC
AspI 253 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
604 GACATCGTC
BanII 123 GGGCCC G[GA]GC[CT]^C
168 GGGCCC
BbsI 472 GAAGAC GAAGAC(2/6)
601 GAAGAC
BglI 148 GCCGCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
170 GCCCCGGCGGC
BpmI 697 CTGGAG CTGGAG(16/14)
793 CTGGAG
BpuAI 472 GAAGAC GAAGAC(2/6)
601 GAAGAC
BsaHI 346 GGCGCC G[GA]^CG[CT]C
354 GACGCC
BsgI 445 CTGCAC GTGCAG(16/14)
339 GTGCAG
BspHI 849 TCATGA T^CATGA
872 TCATGA
BsrI 650 ACTGG ACTGG(1/-1)
792 ACTGG
BstUI 138 CGCG CG^CG
150 CGCG
HaeII 28 AGCGCT [GA]GCGC^[CT]
346 GGCGCC
Hin1I 346 GGCGCC G[GA]^CG[CT]C
354 GACGCC
HinfI 846 GATTC G^A[ACGT]TC
941 GAATC
HphI 159 GGTGA GGTGA(8/7)
654 GGTGA
KspI 137 CCGCGG CCGC^GG
149 CCGCGG
MaeIII 302 GTAAC ^GT[ACGT]AC
949 GTTAC
MslI 687 CATGCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
850 CATGACGGTG
MspA1I 137 CCGCGG C[AC]G^C[GT]G
149 CCGCGG
MvnI 138 CGCG CG^CG
150 CGCG
PflFI 253 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
604 GACATCGTC
PstI 42 CTGCAG CTGCA^G
274 CTGCAG
RcaI 849 TCATGA T^CATGA
872 TCATGA
SacII 137 CCGCGG CCGC^GG
149 CCGCGG
SfaNI 660 GATGC GCATC(5/9)
362 GCATC
TfiI 846 GATTC G^A[AT]TC
941 GAATC
Tth111I 253 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
604 GACATCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI BanI BbvI BcnI
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BsrFI BssKI BstNI Cac8I
CfoI Cfr10I Cfr13I DraII DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
HaeIII HapII HhaI HinP1I HpaII
ItaI MboII MnlI MspI MvaI
MwoI NciI NlaIII NlaIV Sau96I
ScrFI SfcI TaqI TseI TthHB8I