Restriction map for
pFN21AE2084
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BanII BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BlnI BpmI BsaAI BsaI BsaJI
BsaWI BseAI BsgI BsiEI BsiWI
Bsp1407I BspEI BspLU11I BsrBI BsrFI
BsrGI BssHII BstBI BstUI Bsu36I
Cfr10I CpoI DraII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoRV EcoT14I
FbaI FseI FspI KpnI KspI
MluI MroI MslI MspA1I MvnI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tth111I Van91I XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AcyI 37 GGCGCC G[GA]^CG[CT]C
AlwNI 883 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 21 GAGCAC G[AT]GC[AT]^C
BamHI 591 GGATCC G^GATCC
BbeI 37 GGCGCC GGCGC^C
BglII 2196 AGATCT A^GATCT
BlpI 1902 GCTTAGC GC^T[ACGT]AGC
BmyI 21 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 1902 GCTTAGC GC^T[ACGT]AGC
BsaBI 541 GATATATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 37 GGCGCC G[GA]^CG[CT]C
BsiHKAI 21 GAGCAC G[AT]GC[AT]^C
BsmBI 1432 CGTCTC CGTCTC(1/5)
BsmFI 1488 GGGAC GGGAC(10/14)
Bsp1286I 21 GAGCAC G[AGT]GC[ACT]^C
BspDI 548 ATCGAT AT^CGAT
BspMI 1666 ACCTGC ACCTGC(4/8)
BssSI 734 CTCGTG CACGAG(-5/-1)
Bst1107I 1446 GTATAC GTA^TAC
BstAPI 1300 GCAACCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 919 GGTTACC G^GT[ACGT]ACC
BstNI 527 CCAGG CC^[AT]GG
BstPI 919 GGTTACC G^GT[ACGT]ACC
BstZ17I 1446 GTATAC GTA^TAC
CelII 1902 GCTTAGC GC^T[ACGT]AGC
ClaI 548 ATCGAT AT^CGAT
DraIII 483 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 919 GGTTACC G^GT[ACGT]ACC
EcoRII 527 CCAGG ^CC[AT]GG
EcoT22I 1367 ATGCAT ATGCA^T
FauI 111 GCGGG CCCGC(4/6)
HgaI 926 GACGC GACGC(5/10)
Hin1I 37 GGCGCC G[GA]^CG[CT]C
HincII 579 GTTAAC GT[CT]^[GA]AC
HindII 579 GTTAAC GT[CT]^[GA]AC
HpaI 579 GTTAAC GTT^AAC
KasI 37 GGCGCC G^GCGCC
MamI 541 GATATATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1553 CAATTG C^AATTG
MunI 1553 CAATTG C^AATTG
MvaI 527 CCAGG CC^[AT]GG
NarI 37 GGCGCC GG^CGCC
NsiI 1367 ATGCAT ATGCA^T
NspI 1704 GCATGT [GA]CATG^[CT]
PaeR7I 1886 CTCGAG C^TCGAG
PleI 1220 GACTC GAGTC(4/5)
PshAI 908 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfoI 37 GGCGCC GGC^GCC
SmlI 1886 CTCGAG C^T[CT][GA]AG
XhoI 1886 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 1117 GTCTAC GT^[AC][GT]AC
1446 GTATAC
AciI 7 GCGG CCGC(-3/-1)
111 GCGG
AlwI 592 GATCC GGATC(4/5)
591 GGATC
AvaII 27 GGACC G^G[AT]CC
1468 GGTCC
BalI 557 TGGCCA TGG^CCA
979 TGGCCA
BanI 37 GGCGCC G^G[CT][GA]CC
1663 GGCACC
BbsI 1240 GAAGAC GAAGAC(2/6)
1864 GAAGAC
BcnI 683 CCCGG CC^[GC]GG
725 CCCGG
BpuAI 1240 GAAGAC GAAGAC(2/6)
1864 GAAGAC
BsiYI 18 CCCGAGCACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1311 CCTCAAAGTGG
BslI 18 CCCGAGCACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1311 CCTCAAAGTGG
BstXI 462 CCATGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1811 CCAAACTGCTGG
BstYI 591 GGATCC [GA]^GATC[CT]
2196 AGATCT
DraI 627 TTTAAA TTT^AAA
2046 TTTAAA
EaeI 557 TGGCCA [CT]^GGCC[GA]
979 TGGCCA
EarI 72 CTCTTC CTCTTC(1/4)
2073 GAAGAG
HaeII 37 GGCGCC [GA]GCGC^[CT]
96 GGCGCT
HindIII 145 AAGCTT A^AGCTT
1839 AAGCTT
Ksp632I 72 CTCTTC CTCTTC(1/4)
2073 GAAGAG
MaeII 763 ACGT A^CGT
1927 ACGT
MflI 591 GGATCC [GA]^GATC[CT]
2196 AGATCT
MluNI 557 TGGCCA TGG^CCA
979 TGGCCA
MscI 557 TGGCCA TGG^CCA
979 TGGCCA
NciI 683 CCCGG CC^[GC]GG
725 CCCGG
PstI 298 CTGCAG CTGCA^G
1747 CTGCAG
SfcI 298 CTGCAG C^T[GA][CT]AG
1747 CTGCAG
TaqI 549 TCGA T^CGA
1887 TCGA
TspRI 894 CACTG CA[GC]TG[ACGT][ACGT]^
1382 CACTG
TthHB8I 549 TCGA T^CGA
1887 TCGA
XhoII 591 GGATCC [GA]^GATC[CT]
2196 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AcsI AfaI AluI ApoI Asp700I
AvaI BbvI BfaI BseRI BsmAI
BsmI BsoBI BspHI BsrDI BsrI
BssKI BstF5I Cac8I CfoI Cfr13I
DdeI DpnII EcoRI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MnlI MseI MspI
MwoI NdeII NlaIII NlaIV RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
TfiI Tru9I TseI Tsp45I Tsp509I
XcmI XmnI