Restriction map for
pFN21AE4205
##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AscI AseI
AsnI Asp718I AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BlnI BlpI
Bpu1102I BsaI BsaWI BseAI BsiWI
BspDI BspEI BspHI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I CelII
ClaI CpoI DrdI DsaI EagI
EclXI Eco52I EcoT22I FbaI FseI
HincII HindII HindIII HpaI KasI
KpnI KspI MluI MroI MvnI
NarI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspI SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AhdI 1738 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1528 GTGCAC G^TGCAC
AlwNI 1831 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1528 GTGCAC G^TGCAC
AspEI 1738 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 820 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 616 CCCGAG C^[CT]CG[GA]G
BcnI 698 CCGGG CC^[GC]GG
BfaI 1201 CTAG C^TAG
BglII 863 AGATCT A^GATCT
BsaBI 864 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 573 CGACCG CG[GA][CT]^CG
BsmBI 637 CGTCTC CGTCTC(1/5)
BsoBI 616 CCCGAG C^[CT]CG[GA]G
BspLU11I 1870 ACATGT A^CATGT
BsrFI 449 GCCGGC [GA]^CCGG[CT]
BstEII 534 GGTCACC G^GT[ACGT]ACC
BstPI 534 GGTCACC G^GT[ACGT]ACC
BstXI 1337 CCAAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 449 GCCGGC [GA]^CCGG[CT]
DraIII 415 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1738 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 708 CCTCTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 534 GGTCACC G^GT[ACGT]ACC
EcoRV 2074 GATATC GAT^ATC
FauI 1690 CCCGC CCCGC(4/6)
MaeI 1201 CTAG C^TAG
MamI 864 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 449 GCCGGC GCC^GGC
NciI 698 CCGGG CC^[GC]GG
NgoMIV 449 GCCGGC G^CCGGC
PflFI 820 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1830 CCAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 1464 GCTCTTC GCTCTTC(1/4)
ScaI 899 AGTACT AGT^ACT
SexAI 416 ACCTGGT A^CC[AT]GGT
SphI 587 GCATGC GCATG^C
TfiI 1197 GAATC G^A[AT]TC
Tth111I 820 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1830 CCAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1200 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
AcyI 635 GGCGTC G[GA]^CG[CT]C
982 GACGCC
AflIII 1870 ACATGT A^C[GA][CT]GT
2024 ACGTGT
AlwI 217 GATCC GGATC(4/5)
1341 GATCC
AocI 321 CCTCAGG CC^T[ACGT]AGG
1496 CCTCAGG
Aor51HI 647 AGCGCT AGC^GCT
746 AGCGCT
ApaI 700 GGGCCC GGGCC^C
1477 GGGCCC
Asp700I 543 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2160 GAAGCACTTC
AvaII 1350 GGACC G^G[AT]CC
2056 GGTCC
AviII 356 TGCGCA TGC^GCA
1148 TGCGCA
BbsI 1803 GAAGAC GAAGAC(2/6)
1882 GAAGAC
BpuAI 1803 GAAGAC GAAGAC(2/6)
1882 GAAGAC
BsaAI 1420 TACGTG [CT]AC^GT[GA]
2023 TACGTG
BsaHI 635 GGCGTC G[GA]^CG[CT]C
982 GACGCC
BsmAI 1306 GAGAC GTCTC(1/5)
638 GTCTC
BsmFI 1502 GGGAC GGGAC(10/14)
1970 GGGAC
BsmI 1154 GCATTC GAATGC(1/-1)
1321 GCATTC
Bsp1407I 34 TGTACA T^GTACA
1749 TGTACA
BsrDI 908 GCAATG GCAATG(2/0)
2064 GCAATG
BsrGI 34 TGTACA T^GTACA
1749 TGTACA
BstYI 863 AGATCT [GA]^GATC[CT]
1340 AGATCC
Bsu36I 321 CCTCAGG CC^T[ACGT]AGG
1496 CCTCAGG
DraI 1093 TTTAAA TTT^AAA
2029 TTTAAA
DraII 1476 AGGGCCC [GA]G^G[ACGT]CC[CT]
2126 GGGGCCT
EarI 1009 CTCTTC CTCTTC(1/4)
1465 CTCTTC
Eco47III 647 AGCGCT AGC^GCT
746 AGCGCT
Eco81I 321 CCTCAGG CC^T[ACGT]AGG
1496 CCTCAGG
EcoO109I 1476 AGGGCCC [GA]G^G[ACGT]CC[CT]
2126 GGGGCCT
EcoRI 1069 GAATTC G^AATTC
1906 GAATTC
FspI 356 TGCGCA TGC^GCA
1148 TGCGCA
HgaI 982 GACGC GACGC(5/10)
636 GCGTC
Hin1I 635 GGCGTC G[GA]^CG[CT]C
982 GACGCC
Ksp632I 1009 CTCTTC CTCTTC(1/4)
1465 CTCTTC
MfeI 432 CAATTG C^AATTG
1817 CAATTG
MflI 863 AGATCT [GA]^GATC[CT]
1340 AGATCC
MslI 799 CACTCGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1251 CATGAAAGTG
MunI 432 CAATTG C^AATTG
1817 CAATTG
PstI 517 CTGCAG CTGCA^G
1327 CTGCAG
PvuII 514 CAGCTG CAG^CTG
610 CAGCTG
SacI 1462 GAGCTC GAGCT^C
1951 GAGCTC
SspBI 34 TGTACA T^GTACA
1749 TGTACA
XhoII 863 AGATCT [GA]^GATC[CT]
1340 AGATCC
XmnI 543 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2160 GAAGCACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI BalI BanII BbvI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI Bsp1286I BspMI BsrBI
BsrI BssKI BstAPI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI