Restriction map for
pFN21AB9604
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbrPI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BpmI BsaBI BseAI BseRI
BsmBI Bsp1407I BspDI BspEI BspHI
BsrBI BsrGI Bst1107I BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRV FbaI FseI FspI
HincII HindII HindIII HinfI HpaI
KpnI MaeI MaeIII MamI MfeI
MflI MroI MseI MunI NdeI
NheI NspV PacI PaeR7I PflFI
PflMI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI ScaI
SexAI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tsp45I Tsp509I Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AgeI 58 ACCGGT A^CCGGT
AlwI 198 GATCC GGATC(4/5)
AspHI 1054 GAGCTC G[AT]GC[AT]^C
BalI 5 TGGCCA TGG^CCA
BbsI 403 GAAGAC GAAGAC(2/6)
BbvCI 315 CCTCAGC CCTCAGC(-5/-2)
BlpI 963 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 963 GCTCAGC GC^T[ACGT]AGC
BpuAI 403 GAAGAC GAAGAC(2/6)
BsaAI 175 TACGTG [CT]AC^GT[GA]
BsaI 55 GAGACC GGTCTC(1/5)
BsaWI 58 ACCGGT [AT]^CCGG[AT]
BsiHKAI 1054 GAGCTC G[AT]GC[AT]^C
BsmAI 55 GAGAC GTCTC(1/5)
BsmI 1110 GCATTC GAATGC(1/-1)
BspLU11I 407 ACATGT A^CATGT
BspMI 581 ACCTGC ACCTGC(4/8)
BsrDI 1069 CATTGC GCAATG(2/0)
BssHII 1103 GCGCGC G^CGCGC
BssSI 606 CTCGTG CACGAG(-5/-1)
CelII 963 GCTCAGC GC^T[ACGT]AGC
EcoT14I 191 CCATGG C^C[AT][AT]GG
EcoT22I 1066 ATGCAT ATGCA^T
HphI 631 TCACC GGTGA(8/7)
KspI 698 CCGCGG CCGC^GG
MaeII 176 ACGT A^CGT
MluI 777 ACGCGT A^CGCGT
MluNI 5 TGGCCA TGG^CCA
MscI 5 TGGCCA TGG^CCA
NaeI 840 GCCGGC GCC^GGC
NcoI 191 CCATGG C^CATGG
NgoMIV 840 GCCGGC G^CCGGC
NruI 254 TCGCGA TCG^CGA
NsiI 1066 ATGCAT ATGCA^T
NspI 407 ACATGT [GA]CATG^[CT]
PinAI 58 ACCGGT A^CCGGT
PvuII 683 CAGCTG CAG^CTG
SacI 1054 GAGCTC GAGCT^C
SacII 698 CCGCGG CCGC^GG
SapI 495 GCTCTTC GCTCTTC(1/4)
StyI 191 CCATGG C^C[AT][AT]GG
TspRI 1014 CAGTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AflIII 407 ACATGT A^C[GA][CT]GT
777 ACGCGT
AlwNI 671 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
680 CAGCAGCTG
BsgI 895 CTGCAC GTGCAG(16/14)
781 GTGCAG
BsiWI 173 CGTACG C^GTACG
761 CGTACG
BsmFI 127 GGGAC GGGAC(10/14)
1082 GGGAC
BstAPI 670 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
679 GCAGCAGCTGC
BstF5I 303 CATCC GGATG(2/0)
877 CATCC
DdeI 316 CTCAG C^T[ACGT]AG
964 CTCAG
DpnII 198 GATC ^GATC
1123 GATC
FokI 303 CATCC GGATG(9/13)
877 CATCC
HgaI 28 GACGC GACGC(5/10)
384 GACGC
MboI 198 GATC ^GATC
1123 GATC
NdeII 198 GATC ^GATC
1123 GATC
NotI 721 GCGGCCGC GC^GGCCGC
901 GCGGCCGC
Sau3AI 198 GATC ^GATC
1123 GATC
TaqI 413 TCGA T^CGA
1121 TCGA
TthHB8I 413 TCGA T^CGA
1121 TCGA
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# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
ApaI AvaI AvaII BanI BanII
BbeI BbvI BcnI BglI BmyI
BsaHI BsaJI BsiEI BsiYI BslI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DraII DsaI EaeI EagI
EarI EclXI Eco52I EcoO109I EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HhaI Hin1I HinP1I HpaII ItaI
KasI Ksp632I MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NlaIII NlaIV PstI
RsaI Sau96I ScrFI SfaNI SfcI
SfiI SfoI TseI