Restriction map for
pFN21AE3095
##################################
# NO CUT ENZYMES ( 159 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AgeI
AhdI Alw44I AlwNI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AvaI AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstUI BstXI
BstYI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FokI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MfeI MflI MluI
MroI MspA1I MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacII SalI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AflIII 479 ACATGT A^C[GA][CT]GT
AocI 739 CCTCAGG CC^T[ACGT]AGG
ApaI 564 GGGCCC GGGCC^C
AspHI 614 GAGCTC G[AT]GC[AT]^C
AspI 535 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 268 GAAGAC GAAGAC(2/6)
BbvI 597 GCAGC GCAGC(8/12)
BcnI 660 CCGGG CC^[GC]GG
BfaI 784 CTAG C^TAG
BpmI 446 CTGGAG CTGGAG(16/14)
BpuAI 268 GAAGAC GAAGAC(2/6)
BseRI 451 GAGGAG GAGGAG(10/8)
BsiHKAI 614 GAGCTC G[AT]GC[AT]^C
BsiYI 576 CCCCAATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 576 CCCCAATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 608 GTCTC GTCTC(1/5)
BsmFI 662 GGGAC GGGAC(10/14)
BspHI 353 TCATGA T^CATGA
BspLU11I 479 ACATGT A^CATGT
BsrDI 692 CATTGC GCAATG(2/0)
Bsu36I 739 CCTCAGG CC^T[ACGT]AGG
Cac8I 176 GCGAGC GC[ACGT]^[ACGT]GC
Eco81I 739 CCTCAGG CC^T[ACGT]AGG
Fnu4HI 597 GCAGC GC^[ACGT]GC
HapII 660 CCGG C^CGG
HpaII 660 CCGG C^CGG
ItaI 597 GCAGC GC^[ACGT]GC
MaeI 784 CTAG C^TAG
MaeII 536 ACGT A^CGT
MslI 627 CACAGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 660 CCGG C^CGG
MwoI 44 GCTGGTGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 660 CCGGG CC^[GC]GG
NspI 479 ACATGT [GA]CATG^[CT]
PflFI 535 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 748 GAGTC GAGTC(4/5)
PpuMI 289 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 353 TCATGA T^CATGA
SacI 614 GAGCTC GAGCT^C
SapI 696 GCTCTTC GCTCTTC(1/4)
TfiI 360 GATTC G^A[AT]TC
TseI 597 GCAGC G^C[AT]GC
Tth111I 535 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 435 CCAACACAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 380 AAATTC [GA]^AATT[CT]
712 AAATTT
AlwI 507 GATCC GGATC(4/5)
92 GGATC
ApoI 380 AAATTC [GA]^AATT[CT]
712 AAATTT
BalI 302 TGGCCA TGG^CCA
584 TGGCCA
BanII 564 GGGCCC G[GA]GC[CT]^C
614 GAGCTC
BmyI 564 GGGCCC G[AGT]GC[ACT]^C
614 GAGCTC
BsaBI 31 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
87 GATGAGGATC
BsgI 115 CTGCAC GTGCAG(16/14)
483 GTGCAG
Bsp1286I 564 GGGCCC G[AGT]GC[ACT]^C
614 GAGCTC
DraII 289 AGGACCT [GA]G^G[ACGT]CC[CT]
564 GGGCCCC
EaeI 302 TGGCCA [CT]^GGCC[GA]
584 TGGCCA
EcoO109I 289 AGGACCT [GA]G^G[ACGT]CC[CT]
564 GGGCCCC
HinfI 360 GATTC G^A[ACGT]TC
748 GAGTC
MaeIII 541 GTCAC ^GT[ACGT]AC
572 GTGAC
MamI 31 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
87 GATGAGGATC
MluNI 302 TGGCCA TGG^CCA
584 TGGCCA
MscI 302 TGGCCA TGG^CCA
584 TGGCCA
MseI 203 TTAA T^TAA
311 TTAA
SfaNI 319 GATGC GCATC(5/9)
673 GATGC
Tru9I 203 TTAA T^TAA
311 TTAA
Tsp45I 541 GTCAC ^GT[GC]AC
572 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AfaI AluI AvaII BsaJI BsrI
BssKI BstNI Cfr13I DdeI DpnII
DsaI EarI EcoRII EcoT14I HaeIII
HphI Ksp632I MboI MboII MnlI
MvaI NcoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI StyI
TaqI Tsp509I TspRI TthHB8I