Restriction map for
pFN21AE6684
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccI AccII AccIII
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AvrII BamHI BanI BbeI BbrPI
BbvCI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstYI BstZ17I CelII
Cfr10I ClaI CpoI DpnII DraI
DraIII DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV FbaI FseI HapII HgaI
Hin1I HincII HindII HpaI HpaII
KasI KpnI KspI MamI MboI
MfeI MflI MluI MroI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NdeII NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI PstI PvuI
RcaI RsrII SacII SalI SapI
Sau3AI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tth111I XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AclI 337 AACGTT AA^CGTT
AspHI 10 GAGCTC G[AT]GC[AT]^C
AviII 549 TGCGCA TGC^GCA
BalI 464 TGGCCA TGG^CCA
BanII 10 GAGCTC G[GA]GC[CT]^C
BcgI 552 GCAGGCTTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 825 GGATAC GTATCC(6/5)
BmyI 10 GAGCTC G[AGT]GC[ACT]^C
BpmI 13 CTCCAG CTGGAG(16/14)
BseRI 532 GAGGAG GAGGAG(10/8)
BsiHKAI 10 GAGCTC G[AT]GC[AT]^C
BsmI 616 GAATGC GAATGC(1/-1)
Bsp1286I 10 GAGCTC G[AGT]GC[ACT]^C
BstXI 141 CCACAGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 499 GACACCGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 464 TGGCCA [CT]^GGCC[GA]
EcoT14I 666 CCAAGG C^C[AT][AT]GG
FspI 549 TGCGCA TGC^GCA
HaeII 806 GGCGCT [GA]GCGC^[CT]
HindIII 837 AAGCTT A^AGCTT
MluNI 464 TGGCCA TGG^CCA
MscI 464 TGGCCA TGG^CCA
MseI 971 TTAA T^TAA
MspA1I 81 CAGCTG C[AC]G^C[GT]G
PleI 214 GAGTC GAGTC(4/5)
PpuMI 962 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 337 AACGTT AA^CGTT
PvuII 81 CAGCTG CAG^CTG
SacI 10 GAGCTC GAGCT^C
SspI 579 AATATT AAT^ATT
StyI 666 CCAAGG C^C[AT][AT]GG
TaqI 795 TCGA T^CGA
TfiI 274 GATTC G^A[AT]TC
Tru9I 971 TTAA T^TAA
Tsp509I 646 AATT ^AATT
TthHB8I 795 TCGA T^CGA
XbaI 162 TCTAGA T^CTAGA
XcmI 385 CCATTGTTTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AfaI 399 GTAC GT^AC
564 GTAC
AlwNI 118 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
989 CAGTGACTG
AocI 529 CCTGAGG CC^T[ACGT]AGG
738 CCTCAGG
AvaII 963 GGACC G^G[AT]CC
1006 GGTCC
BbsI 376 GAAGAC GAAGAC(2/6)
715 GAAGAC
BfaI 163 CTAG C^TAG
569 CTAG
BpuAI 376 GAAGAC GAAGAC(2/6)
715 GAAGAC
BsaI 166 GAGACC GGTCTC(1/5)
198 GGTCTC
Bsu36I 529 CCTGAGG CC^T[ACGT]AGG
738 CCTCAGG
DraII 669 AGGCCCT [GA]G^G[ACGT]CC[CT]
962 GGGACCT
EarI 88 CTCTTC CTCTTC(1/4)
346 CTCTTC
Eco81I 529 CCTGAGG CC^T[ACGT]AGG
738 CCTCAGG
EcoO109I 669 AGGCCCT [GA]G^G[ACGT]CC[CT]
962 GGGACCT
EcoT22I 452 ATGCAT ATGCA^T
618 ATGCAT
FauI 458 CCCGC CCCGC(4/6)
891 GCGGG
HinfI 214 GAGTC G^A[ACGT]TC
274 GATTC
Ksp632I 88 CTCTTC CTCTTC(1/4)
346 CTCTTC
MaeI 163 CTAG C^TAG
569 CTAG
MaeII 147 ACGT A^CGT
338 ACGT
NsiI 452 ATGCAT ATGCA^T
618 ATGCAT
PflMI 117 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
467 CCATCATCTGG
RsaI 399 GTAC GT^AC
564 GTAC
Tsp45I 31 GTCAC ^GT[GC]AC
991 GTGAC
Van91I 117 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
467 CCATCATCTGG
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# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsiYI
BslI BsmAI BsmFI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI EcoRII Fnu4HI FokI HaeIII
HhaI HinP1I HphI ItaI MaeIII
MboII MnlI MslI MvaI MwoI
NlaIII NlaIV Sau96I ScrFI SfaNI
TseI TspRI