Restriction map for
pFN21AE6612
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AccIII AciI
AclI AcsI AcyI AflII AgeI
AhdI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BbeI BbrPI
BbsI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsmFI BsmI BsoBI BspDI
BspEI BspHI BspMI BsrBI BsrDI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeII MfeI MluI MroI
MunI NarI NciI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tth111I XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 174 CGCG CG^CG
AflIII 534 ACATGT A^C[GA][CT]GT
Alw44I 568 GTGCAC G^TGCAC
ApaI 478 GGGCCC GGGCC^C
ApaLI 568 GTGCAC G^TGCAC
BalI 95 TGGCCA TGG^CCA
BamHI 248 GGATCC G^GATCC
BanI 523 GGTGCC G^G[CT][GA]CC
BbvCI 362 CCTCAGC CCTCAGC(-5/-2)
BsaBI 426 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI 900 GTCTC GTCTC(1/5)
Bsp1407I 230 TGTACA T^GTACA
BspLU11I 534 ACATGT A^CATGT
BsrFI 773 GCCGGC [GA]^CCGG[CT]
BsrGI 230 TGTACA T^GTACA
BssSI 234 CACGAG CACGAG(-5/-1)
BstAPI 257 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 174 CGCG CG^CG
BstXI 689 CCATTCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 773 GCCGGC [GA]^CCGG[CT]
EcoNI 503 CCTACTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 581 CCATGG C^C[AT][AT]GG
HaeII 646 AGCGCC [GA]GCGC^[CT]
HapII 774 CCGG C^CGG
HinfI 69 GATTC G^A[ACGT]TC
HpaII 774 CCGG C^CGG
MaeIII 295 GTCAC ^GT[ACGT]AC
MamI 426 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 25 GAAGA GAAGA(8/7)
MluNI 95 TGGCCA TGG^CCA
MscI 95 TGGCCA TGG^CCA
MslI 888 CACGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 774 CCGG C^CGG
MvnI 174 CGCG CG^CG
NaeI 773 GCCGGC GCC^GGC
NcoI 581 CCATGG C^CATGG
NgoMIV 773 GCCGGC G^CCGGC
NspI 534 ACATGT [GA]CATG^[CT]
PflMI 254 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 858 CTGCAG CTGCA^G
ScaI 785 AGTACT AGT^ACT
SexAI 452 ACCTGGT A^CC[AT]GGT
SfcI 858 CTGCAG C^T[GA][CT]AG
SspBI 230 TGTACA T^GTACA
StyI 581 CCATGG C^C[AT][AT]GG
TaqI 203 TCGA T^CGA
TfiI 69 GATTC G^A[AT]TC
Tsp45I 295 GTCAC ^GT[GC]AC
Tsp509I 835 AATT ^AATT
TthHB8I 203 TCGA T^CGA
Van91I 254 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 21 TCTAGA T^CTAGA
XcmI 751 CCACTGTTCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AocI 904 CCTGAGG CC^T[ACGT]AGG
915 CCTCAGG
AspHI 264 GTGCTC G[AT]GC[AT]^C
568 GTGCAC
BfaI 22 CTAG C^TAG
574 CTAG
BsiHKAI 264 GTGCTC G[AT]GC[AT]^C
568 GTGCAC
BstYI 248 GGATCC [GA]^GATC[CT]
926 GGATCT
Bsu36I 904 CCTGAGG CC^T[ACGT]AGG
915 CCTCAGG
Cac8I 105 GCCTGC GC[ACGT]^[ACGT]GC
773 GCCGGC
CfoI 175 GCGC GCG^C
647 GCGC
EaeI 95 TGGCCA [CT]^GGCC[GA]
311 CGGCCA
Eco81I 904 CCTGAGG CC^T[ACGT]AGG
915 CCTCAGG
HhaI 175 GCGC GCG^C
647 GCGC
HinP1I 175 GCGC G^CGC
647 GCGC
MaeI 22 CTAG C^TAG
574 CTAG
MflI 248 GGATCC [GA]^GATC[CT]
926 GGATCT
MseI 392 TTAA T^TAA
850 TTAA
MspA1I 258 CAGCTG C[AC]G^C[GT]G
855 CAGCTG
PpuMI 279 AGGTCCT [GA]G^G[AT]CC[CT]
470 AGGACCT
PvuII 258 CAGCTG CAG^CTG
855 CAGCTG
Tru9I 392 TTAA T^TAA
850 TTAA
XhoII 248 GGATCC [GA]^GATC[CT]
926 GGATCT
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# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AfaI AluI AlwI AlwNI AvaII
BanII BbvI BmyI BpmI BsaJI
BseRI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII DraII DsaI EcoO109I EcoRII
Fnu4HI FokI HaeIII HphI ItaI
MboI MnlI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI TseI TspRI