##################################
# NO CUT ENZYMES (  225 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BanII       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaJI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmAI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BsrI        BssHII      
BssKI       BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstNI       BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRII      EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
HpaII       HphI        KasI        KpnI        Ksp632I     
KspI        MaeI        MaeII       MaeIII      MamI        
MfeI        MluI        MluNI       MroI        MscI        
MseI        MspA1I      MspI        MunI        MvaI        
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        Sau96I      SbfI        ScaI        ScrFI       
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TaqI        Tru9I       Tsp45I      
Tsp509I     Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AfaI          189  GTAC                 GT^AC
AlwI           29  GGATC                GGATC(4/5)
AlwNI         140  CAGAATCTG            CAG[ACGT][ACGT][ACGT]^CTG
BanI          133  GGCACC               G^G[CT][GA]CC
BbsI           61  GAAGAC               GAAGAC(2/6)
BbvI           69  GCTGC                GCAGC(8/12)
BpuAI          61  GAAGAC               GAAGAC(2/6)
BsiYI         208  CCCCCCATTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          208  CCCCCCATTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BstF5I         81  GGATG                GGATG(2/0)
BstYI          29  GGATCT               [GA]^GATC[CT]
DdeI          177  CTTAG                C^T[ACGT]AG
DpnII          30  GATC                 ^GATC
Fnu4HI         69  GCTGC                GC^[ACGT]GC
FokI           81  GGATG                GGATG(9/13)
HinfI         142  GAATC                G^A[ACGT]TC
ItaI           69  GCTGC                GC^[ACGT]GC
MboI           30  GATC                 ^GATC
MboII          61  GAAGA                GAAGA(8/7)
MflI           29  GGATCT               [GA]^GATC[CT]
MslI          196  CATGAGAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII          30  GATC                 ^GATC
NlaIII        196  CATG                 CATG^
NlaIV         133  GGCACC               GG[ACGT]^[ACGT]CC
RsaI          189  GTAC                 GT^AC
Sau3AI         30  GATC                 ^GATC
SfaNI          49  GATGC                GCATC(5/9)
TfiI          142  GAATC                G^A[AT]TC
TseI           69  GCTGC                G^C[AT]GC
XhoII          29  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    2 enzymes) #
###################################

AluI           68  AGCT                 AG^CT
              104  AGCT                
TspRI           6  CAGTG                CA[GC]TG[ACGT][ACGT]^
               95  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (    1 enzymes) #
#########################################

MnlI