Restriction map for
pFN21AE5423
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraII DraIII
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HindIII HpaI KpnI
KspI MaeI MfeI MluI MluNI
MroI MscI MslI MspA1I MunI
MvnI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SapI SbfI ScaI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TspRI Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AccI 47 GTCGAC GT^[AC][GT]AC
AciI 511 CCGC CCGC(-3/-1)
AcsI 487 GAATTT [GA]^AATT[CT]
AcyI 31 GGCGCC G[GA]^CG[CT]C
AflIII 1007 ACGTGT A^C[GA][CT]GT
AlwI 924 GGATC GGATC(4/5)
ApaI 78 GGGCCC GGGCC^C
ApoI 487 GAATTT [GA]^AATT[CT]
AvaII 329 GGACC G^G[AT]CC
BanI 31 GGCGCC G^G[CT][GA]CC
BanII 78 GGGCCC G[GA]GC[CT]^C
BbeI 31 GGCGCC GGCGC^C
BbsI 119 GTCTTC GAAGAC(2/6)
BmyI 78 GGGCCC G[AGT]GC[ACT]^C
BpmI 719 CTGGAG CTGGAG(16/14)
BpuAI 119 GTCTTC GAAGAC(2/6)
BsaBI 148 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 31 GGCGCC G[GA]^CG[CT]C
BseRI 572 CTCCTC GAGGAG(10/8)
Bsp1286I 78 GGGCCC G[AGT]GC[ACT]^C
BspHI 653 TCATGA T^CATGA
BspMI 24 GCAGGT ACCTGC(4/8)
BsrI 733 ACTGG ACTGG(1/-1)
BstYI 924 GGATCT [GA]^GATC[CT]
DdeI 885 CTTAG C^T[ACGT]AG
DpnII 925 GATC ^GATC
DraI 670 TTTAAA TTT^AAA
DrdI 376 GACTGTCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 250 CCATGG C^C[GA][CT]GG
EarI 973 GAAGAG CTCTTC(1/4)
HaeII 31 GGCGCC [GA]GCGC^[CT]
HgaI 21 GACGC GACGC(5/10)
Hin1I 31 GGCGCC G[GA]^CG[CT]C
HincII 47 GTCGAC GT[CT]^[GA]AC
HindII 47 GTCGAC GT[CT]^[GA]AC
HphI 736 GGTGA GGTGA(8/7)
KasI 31 GGCGCC G^GCGCC
Ksp632I 973 GAAGAG CTCTTC(1/4)
MamI 148 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 925 GATC ^GATC
MflI 924 GGATCT [GA]^GATC[CT]
NarI 31 GGCGCC GG^CGCC
NcoI 250 CCATGG C^CATGG
NdeII 925 GATC ^GATC
NspI 348 GCATGT [GA]CATG^[CT]
PflMI 36 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 21 GACGCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 653 TCATGA T^CATGA
SalI 47 GTCGAC G^TCGAC
Sau3AI 925 GATC ^GATC
SexAI 273 ACCAGGT A^CC[AT]GGT
SfcI 437 CTGTAG C^T[GA][CT]AG
SfoI 31 GGCGCC GGC^GCC
SmaI 81 CCCGGG CCC^GGG
TaqI 48 TCGA T^CGA
TfiI 901 GATTC G^A[AT]TC
TthHB8I 48 TCGA T^CGA
Van91I 36 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 708 CCACAGGAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 924 GGATCT [GA]^GATC[CT]
XmaCI 81 CCCGGG C^CCGGG
XmaI 81 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AlwNI 37 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
747 CAGAGTCTG
AvaI 52 CTCGGG C^[CT]CG[GA]G
81 CCCGGG
BsaI 714 GAGACC GGTCTC(1/5)
192 GGTCTC
BsiYI 36 CCAGGTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
75 CCTGGGCCCGG
BslI 36 CCAGGTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
75 CCTGGGCCCGG
BsmAI 714 GAGAC GTCTC(1/5)
193 GTCTC
BsmFI 56 GGGAC GGGAC(10/14)
172 GTCCC
BsoBI 52 CTCGGG C^[CT]CG[GA]G
81 CCCGGG
CfoI 32 GCGC GCG^C
1024 GCGC
HhaI 32 GCGC GCG^C
1024 GCGC
HinP1I 32 GCGC G^CGC
1024 GCGC
MaeIII 355 GTCAC ^GT[ACGT]AC
616 GTCAC
PleI 50 GACTC GAGTC(4/5)
749 GAGTC
SfaNI 318 GATGC GCATC(5/9)
153 GCATC
Tsp45I 355 GTCAC ^GT[GC]AC
616 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AfaI AluI BbvI BcnI BsaJI
BssKI BstF5I BstNI Cac8I Cfr13I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII ItaI MaeII
MboII MnlI MseI MspI MvaI
MwoI NciI NlaIII NlaIV RsaI
Sau96I ScrFI StyI Tru9I TseI
Tsp509I