Restriction map for
pFN21AE9464
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AocI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaHI BsaI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI BspDI BspEI BspHI BspLU11I
BspMI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HgaI Hin1I
HincII HindII HpaI KasI KpnI
KspI MaeI MfeI MluI MroI
MunI MvnI NarI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TfiI
Tsp509I Tth111I Van91I XbaI XcmI
XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccI 610 GTCTAC GT^[AC][GT]AC
AflIII 686 ACGTGT A^C[GA][CT]GT
BbsI 106 GAAGAC GAAGAC(2/6)
BcgI 1004 GCACGCAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 298 GTATCC GTATCC(6/5)
BclI 395 TGATCA T^GATCA
BpuAI 106 GAAGAC GAAGAC(2/6)
BsaWI 128 TCCGGT [AT]^CCGG[AT]
BsmAI 898 GAGAC GTCTC(1/5)
Bsp1407I 915 TGTACA T^GTACA
BsrBI 817 CCGCTC CCGCTC(-3/-3)
BsrDI 282 CATTGC GCAATG(2/0)
BsrFI 1021 GCCGGC [GA]^CCGG[CT]
BsrGI 915 TGTACA T^GTACA
Cfr10I 1021 GCCGGC [GA]^CCGG[CT]
EcoT14I 561 CCATGG C^C[AT][AT]GG
FbaI 395 TGATCA T^GATCA
HindIII 190 AAGCTT A^AGCTT
HinfI 1015 GACTC G^A[ACGT]TC
MseI 548 TTAA T^TAA
NaeI 1021 GCCGGC GCC^GGC
NcoI 561 CCATGG C^CATGG
NgoMIV 1021 GCCGGC G^CCGGC
PaeR7I 999 CTCGAG C^TCGAG
PleI 1015 GACTC GAGTC(4/5)
SapI 165 GCTCTTC GCTCTTC(1/4)
SfiI 559 GGCCATGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 287 CCCGGG CCC^GGG
SmlI 999 CTCGAG C^T[CT][GA]AG
SspBI 915 TGTACA T^GTACA
StyI 561 CCATGG C^C[AT][AT]GG
Tru9I 548 TTAA T^TAA
XhoI 999 CTCGAG C^TCGAG
XmaCI 287 CCCGGG C^CCGGG
XmaI 287 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Aor51HI 42 AGCGCT AGC^GCT
1097 AGCGCT
BanI 60 GGCACC G^G[CT][GA]CC
115 GGCACC
BanII 766 GGGCTC G[GA]GC[CT]^C
859 GAGCCC
BbvCI 983 CCTCAGC CCTCAGC(-5/-2)
538 GCTGAGG
BglI 560 GCCATGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1064 GCCACACTGGC
BsaAI 685 TACGTG [CT]AC^GT[GA]
826 TACGTG
BsaBI 213 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
523 GATGCCCATC
BstAPI 328 GCAACTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
967 GCAGGGGCTGC
DsaI 293 CCGTGG C^C[GA][CT]GG
561 CCATGG
EarI 166 CTCTTC CTCTTC(1/4)
871 CTCTTC
Eco47III 42 AGCGCT AGC^GCT
1097 AGCGCT
EcoNI 9 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
717 CCTACTGCAGG
Ksp632I 166 CTCTTC CTCTTC(1/4)
871 CTCTTC
MaeII 686 ACGT A^CGT
827 ACGT
MaeIII 19 GTGAC ^GT[ACGT]AC
490 GTCAC
MamI 213 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
523 GATGCCCATC
PpuMI 137 GGGACCT [GA]G^G[AT]CC[CT]
499 GGGACCC
PstI 721 CTGCAG CTGCA^G
965 CTGCAG
PvuII 536 CAGCTG CAG^CTG
986 CAGCTG
SfcI 721 CTGCAG C^T[GA][CT]AG
965 CTGCAG
Tsp45I 19 GTGAC ^GT[GC]AC
490 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BalI BbvI
BcnI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI TaqI TseI TspRI TthHB8I
XhoII