Restriction map for
pFN21AE3287
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# NO CUT ENZYMES ( 120 enzymes) #
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AatII AccI AccIII AclI AcyI
AflII AgeI AhdI AocI Aor51HI
ApaI AscI AseI AsnI AspEI
AspI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaHI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BspEI
BspLU11I BsrDI BsrFI BssHII Bst1107I
BstAPI BstBI BstZ17I Bsu36I CelII
Cfr10I CpoI DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I FbaI FseI FspI
HaeII HgaI Hin1I HpaI KasI
Ksp632I KspI MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI Tth111I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 258 GGTACC G^GTACC
AccII 2077 CGCG CG^CG
AflIII 2184 ACGTGT A^C[GA][CT]GT
Alw44I 120 GTGCAC G^TGCAC
AlwI 2167 GATCC GGATC(4/5)
AlwNI 571 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 120 GTGCAC G^TGCAC
Asp700I 1532 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 258 GGTACC G^GTACC
BbsI 1536 GTCTTC GAAGAC(2/6)
BpmI 76 CTGGAG CTGGAG(16/14)
BpuAI 1536 GTCTTC GAAGAC(2/6)
BsaBI 343 GATATGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 291 GAGACC GGTCTC(1/5)
BsmI 1250 GCATTC GAATGC(1/-1)
BspDI 1312 ATCGAT AT^CGAT
BspHI 1569 TCATGA T^CATGA
BsrBI 28 CCGCTC CCGCTC(-3/-3)
BstEII 853 GGTGACC G^GT[ACGT]ACC
BstPI 853 GGTGACC G^GT[ACGT]ACC
BstUI 2077 CGCG CG^CG
BstXI 248 CCATTTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 1312 ATCGAT AT^CGAT
DraI 736 TTTAAA TTT^AAA
EcoO65I 853 GGTGACC G^GT[ACGT]ACC
EcoRI 1738 GAATTC G^AATTC
EcoRV 1185 GATATC GAT^ATC
EcoT22I 346 ATGCAT ATGCA^T
HincII 229 GTTGAC GT[CT]^[GA]AC
HindII 229 GTTGAC GT[CT]^[GA]AC
HindIII 1076 AAGCTT A^AGCTT
KpnI 258 GGTACC GGTAC^C
MaeII 2184 ACGT A^CGT
MamI 343 GATATGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 2077 CGCG CG^CG
NsiI 346 ATGCAT ATGCA^T
PpuMI 1033 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 1569 TCATGA T^CATGA
SacI 93 GAGCTC GAGCT^C
SfcI 1177 CTACAG C^T[GA][CT]AG
SmaI 150 CCCGGG CCC^GGG
SphI 1293 GCATGC GCATG^C
StuI 1646 AGGCCT AGG^CCT
XcmI 1797 CCAGACTTCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 150 CCCGGG C^CCGGG
XmaI 150 CCCGGG C^CCGGG
XmnI 1532 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AvaII 1034 GGACC G^G[AT]CC
2143 GGACC
BanII 93 GAGCTC G[GA]GC[CT]^C
1629 GGGCTC
BfaI 479 CTAG C^TAG
884 CTAG
BglII 398 AGATCT A^GATCT
1281 AGATCT
Bsp1407I 44 TGTACA T^GTACA
1440 TGTACA
BsrGI 44 TGTACA T^GTACA
1440 TGTACA
BstF5I 1432 CATCC GGATG(2/0)
2129 CATCC
BstYI 398 AGATCT [GA]^GATC[CT]
1281 AGATCT
CfoI 15 GCGC GCG^C
1745 GCGC
DraII 1033 GGGACCC [GA]G^G[ACGT]CC[CT]
2035 AGGGCCT
DraIII 468 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
1444 CACTTTGTG
EcoNI 996 CCTTATAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1494 CCTTGCAAAGG
EcoO109I 1033 GGGACCC [GA]G^G[ACGT]CC[CT]
2035 AGGGCCT
FokI 1432 CATCC GGATG(9/13)
2129 CATCC
HhaI 15 GCGC GCG^C
1745 GCGC
HinP1I 15 GCGC G^CGC
1745 GCGC
MaeI 479 CTAG C^TAG
884 CTAG
MflI 398 AGATCT [GA]^GATC[CT]
1281 AGATCT
MseI 737 TTAA T^TAA
1021 TTAA
SmlI 840 CTCAAG C^T[CT][GA]AG
1624 CTCAAG
SspBI 44 TGTACA T^GTACA
1440 TGTACA
Tru9I 737 TTAA T^TAA
1021 TTAA
XhoII 398 AGATCT [GA]^GATC[CT]
1281 AGATCT
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI AvaI BanI BbvI BcnI
BmyI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BssSI BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
EcoT14I FauI Fnu4HI HaeIII HapII
HinfI HpaII HphI ItaI MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I