Restriction map for
pFN21AE1779
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# NO CUT ENZYMES ( 172 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BamHI BanI BbeI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaHI
BsaI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoT22I FbaI FseI
FspI HaeII Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MspA1I MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmeI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI TaqI TspRI Tth111I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AciI 381 GCGG CCGC(-3/-1)
AspHI 586 GAGCTC G[AT]GC[AT]^C
AvaI 253 CCCGAG C^[CT]CG[GA]G
AvaII 269 GGACC G^G[AT]CC
BanII 586 GAGCTC G[GA]GC[CT]^C
BbrPI 109 CACGTG CAC^GTG
BfaI 434 CTAG C^TAG
BpmI 494 CTGGAG CTGGAG(16/14)
BsaAI 109 CACGTG [CT]AC^GT[GA]
BsaWI 266 ACCGGA [AT]^CCGG[AT]
BsiHKAI 586 GAGCTC G[AT]GC[AT]^C
BsmAI 647 GTCTC GTCTC(1/5)
BsmFI 236 GGGAC GGGAC(10/14)
BsoBI 253 CCCGAG C^[CT]CG[GA]G
BspMI 725 ACCTGC ACCTGC(4/8)
BstYI 573 AGATCC [GA]^GATC[CT]
CfoI 318 GCGC GCG^C
DrdI 222 GACTATGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 169 GAAGAG CTCTTC(1/4)
EcoRV 280 GATATC GAT^ATC
EcoT14I 116 CCAAGG C^C[AT][AT]GG
FauI 381 GCGGG CCCGC(4/6)
HapII 267 CCGG C^CGG
HgaI 370 GACGC GACGC(5/10)
HhaI 318 GCGC GCG^C
HinP1I 318 GCGC G^CGC
HinfI 675 GAATC G^A[ACGT]TC
HpaII 267 CCGG C^CGG
Ksp632I 169 GAAGAG CTCTTC(1/4)
MaeI 434 CTAG C^TAG
MflI 573 AGATCC [GA]^GATC[CT]
MspI 267 CCGG C^CGG
MwoI 63 GCTCTTGGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PmaCI 109 CACGTG CAC^GTG
PmlI 109 CACGTG CAC^GTG
SacI 586 GAGCTC GAGCT^C
SapI 169 GAAGAGC GCTCTTC(1/4)
SspI 466 AATATT AAT^ATT
StyI 116 CCAAGG C^C[AT][AT]GG
TfiI 675 GAATC G^A[AT]TC
XcmI 299 CCATTGTGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 573 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 452 AAATTC [GA]^AATT[CT]
767 GAATTT
AfaI 258 GTAC GT^AC
853 GTAC
AlwI 306 GATCC GGATC(4/5)
574 GATCC
ApoI 452 AAATTC [GA]^AATT[CT]
767 GAATTT
BbvI 602 GCAGC GCAGC(8/12)
782 GCTGC
BmyI 333 GGGCAC G[AGT]GC[ACT]^C
586 GAGCTC
BsaJI 116 CCAAGG C^C[ACGT][ACGT]GG
737 CCTGGG
BsiYI 683 CCTTCAACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
732 CCCATCCTGGG
BslI 683 CCTTCAACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
732 CCCATCCTGGG
Bsp1286I 333 GGGCAC G[AGT]GC[ACT]^C
586 GAGCTC
BstF5I 32 CATCC GGATG(2/0)
734 CATCC
Cac8I 72 GCTGGC GC[ACGT]^[ACGT]GC
174 GCTGGC
DdeI 584 CTGAG C^T[ACGT]AG
756 CTCAG
DpnII 306 GATC ^GATC
574 GATC
Fnu4HI 602 GCAGC GC^[ACGT]GC
782 GCTGC
FokI 32 CATCC GGATG(9/13)
734 CATCC
HphI 28 TCACC GGTGA(8/7)
513 TCACC
ItaI 602 GCAGC GC^[ACGT]GC
782 GCTGC
MaeII 110 ACGT A^CGT
403 ACGT
MboI 306 GATC ^GATC
574 GATC
MslI 32 CATCCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
338 CATACGAGTG
NdeII 306 GATC ^GATC
574 GATC
NlaIII 240 CATG CATG^
642 CATG
RsaI 258 GTAC GT^AC
853 GTAC
Sau3AI 306 GATC ^GATC
574 GATC
SfaNI 809 GATGC GCATC(5/9)
188 GCATC
TseI 602 GCAGC G^C[AT]GC
782 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AluI BsrI BssKI BstNI Cfr13I
EcoRII HaeIII MaeIII MboII MnlI
MseI MvaI Sau96I ScrFI Tru9I
Tsp45I Tsp509I