Restriction map for
pFN21AE6363
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccII AciI AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII BanII BbeI BbrPI BbvCI
BbvI BcgI BciVI BfrI BglI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaI BsgI BsiEI BsiWI BsmAI
BsmBI BsoBI BspDI BspMI BsrBI
BsrFI BssHII BssSI BstAPI BstBI
BstUI BstXI Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRV EcoT22I
FauI Fnu4HI FseI FspI HaeII
HhaI HinP1I HindIII HpaI ItaI
KasI KpnI KspI MamI MluI
MspA1I MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SspI StuI SwaI TaqI
TfiI TseI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AccI 51 GTATAC GT^[AC][GT]AC
AccIII 1140 TCCGGA T^CCGGA
AcyI 431 GGCGTC G[GA]^CG[CT]C
AfaI 614 GTAC GT^AC
AflIII 986 ACATGT A^C[GA][CT]GT
AlwNI 1034 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 264 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 168 GTGCTC G[AT]GC[AT]^C
AvaII 290 GGTCC G^G[AT]CC
AvrII 1153 CCTAGG C^CTAGG
BalI 900 TGGCCA TGG^CCA
BanI 67 GGTGCC G^G[CT][GA]CC
BbsI 331 GTCTTC GAAGAC(2/6)
BclI 870 TGATCA T^GATCA
BcnI 497 CCGGG CC^[GC]GG
BglII 1173 AGATCT A^GATCT
BlnI 1153 CCTAGG C^CTAGG
BmyI 168 GTGCTC G[AGT]GC[ACT]^C
BpuAI 331 GTCTTC GAAGAC(2/6)
BsaHI 431 GGCGTC G[GA]^CG[CT]C
BsaWI 1140 TCCGGA [AT]^CCGG[AT]
BseAI 1140 TCCGGA T^CCGGA
BseRI 858 GAGGAG GAGGAG(10/8)
BsiHKAI 168 GTGCTC G[AT]GC[AT]^C
BsiYI 951 CCTTTCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 951 CCTTTCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 168 GTGCTC G[AGT]GC[ACT]^C
Bsp1407I 613 TGTACA T^GTACA
BspEI 1140 TCCGGA T^CCGGA
BspHI 281 TCATGA T^CATGA
BspLU11I 986 ACATGT A^CATGT
BsrDI 78 CATTGC GCAATG(2/0)
BsrGI 613 TGTACA T^GTACA
Bst1107I 51 GTATAC GTA^TAC
BstEII 1011 GGTTACC G^GT[ACGT]ACC
BstPI 1011 GGTTACC G^GT[ACGT]ACC
BstZ17I 51 GTATAC GTA^TAC
DsaI 362 CCATGG C^C[GA][CT]GG
EaeI 900 TGGCCA [CT]^GGCC[GA]
EcoO65I 1011 GGTTACC G^GT[ACGT]ACC
EcoRI 268 GAATTC G^AATTC
FbaI 870 TGATCA T^GATCA
HgaI 432 GCGTC GACGC(5/10)
Hin1I 431 GGCGTC G[GA]^CG[CT]C
HincII 42 GTCAAC GT[CT]^[GA]AC
HindII 42 GTCAAC GT[CT]^[GA]AC
HphI 180 GGTGA GGTGA(8/7)
MaeII 531 ACGT A^CGT
MfeI 609 CAATTG C^AATTG
MluNI 900 TGGCCA TGG^CCA
MroI 1140 TCCGGA T^CCGGA
MscI 900 TGGCCA TGG^CCA
MunI 609 CAATTG C^AATTG
NciI 497 CCGGG CC^[GC]GG
NcoI 362 CCATGG C^CATGG
NspI 986 ACATGT [GA]CATG^[CT]
RcaI 281 TCATGA T^CATGA
RsaI 614 GTAC GT^AC
SapI 403 GCTCTTC GCTCTTC(1/4)
SfcI 15 CTGTAG C^T[GA][CT]AG
SpeI 105 ACTAGT A^CTAGT
SspBI 613 TGTACA T^GTACA
Tsp45I 674 GTCAC ^GT[GC]AC
XmnI 264 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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BamHI 443 GGATCC G^GATCC
1166 GGATCC
BsmFI 499 GGGAC GGGAC(10/14)
596 GTCCC
BsmI 824 GCATTC GAATGC(1/-1)
940 GCATTC
Cac8I 1123 GCCAGC GC[ACGT]^[ACGT]GC
1127 GCAAGC
DdeI 171 CTCAG C^T[ACGT]AG
1177 CTAAG
DraII 947 AGGGCCT [GA]G^G[ACGT]CC[CT]
1060 AGGCCCT
EarI 404 CTCTTC CTCTTC(1/4)
426 GAAGAG
EcoO109I 947 AGGGCCT [GA]G^G[ACGT]CC[CT]
1060 AGGCCCT
EcoT14I 362 CCATGG C^C[AT][AT]GG
1153 CCTAGG
HapII 497 CCGG C^CGG
1141 CCGG
HinfI 843 GACTC G^A[ACGT]TC
1252 GAGTC
HpaII 497 CCGG C^CGG
1141 CCGG
Ksp632I 404 CTCTTC CTCTTC(1/4)
426 GAAGAG
MslI 541 CATCACGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1029 CACAACAGTG
MspI 497 CCGG C^CGG
1141 CCGG
PleI 843 GACTC GAGTC(4/5)
1252 GAGTC
SmlI 90 CTCAAG C^T[CT][GA]AG
1133 CTCAAG
StyI 362 CCATGG C^C[AT][AT]GG
1153 CCTAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AcsI AluI AlwI ApoI BfaI
BsaJI BsrI BssKI BstF5I BstNI
BstYI Cfr13I DpnII EcoRII FokI
HaeIII MaeI MaeIII MboI MboII
MflI MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI Tru9I Tsp509I TspRI
XhoII