Restriction map for
pFN21AE9426
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AflII AgeI AhdI ApaI AscI
Asp718I AspEI AvaI AviII AvrII
BalI BanI BanII BbeI BbrPI
BbvCI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaI BseAI BsiWI BsmBI BsmI
BsoBI BspDI BspEI BspHI BsrBI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
DsaI Eam1105I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FseI FspI
Hin1I HpaI KasI KpnI KspI
MluI MluNI MroI MscI NaeI
NarI NciI NcoI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
StuI StyI SwaI Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AclI 1315 AACGTT AA^CGTT
AflIII 310 ACATGT A^C[GA][CT]GT
AlwNI 1966 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1639 AGCGCT AGC^GCT
Asp700I 581 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1090 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 104 GGATCC G^GATCC
BciVI 321 GGATAC GTATCC(6/5)
BclI 98 TGATCA T^GATCA
BpmI 87 CTGGAG CTGGAG(16/14)
BsaWI 353 TCCGGT [AT]^CCGG[AT]
BseRI 1447 CTCCTC GAGGAG(10/8)
BsiEI 2254 CGGCCG CG[GA][CT]^CG
BsiYI 328 CCAAATGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 328 CCAAATGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspLU11I 310 ACATGT A^CATGT
BspMI 444 GCAGGT ACCTGC(4/8)
BsrFI 1808 ACCGGC [GA]^CCGG[CT]
BstYI 104 GGATCC [GA]^GATC[CT]
Cfr10I 1808 ACCGGC [GA]^CCGG[CT]
DraII 804 GGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 2254 CGGCCG [CT]^GGCC[GA]
EagI 2254 CGGCCG C^GGCCG
EclXI 2254 CGGCCG C^GGCCG
Eco47III 1639 AGCGCT AGC^GCT
Eco52I 2254 CGGCCG C^GGCCG
EcoO109I 804 GGGGCCT [GA]G^G[ACGT]CC[CT]
FauI 334 GCGGG CCCGC(4/6)
FbaI 98 TGATCA T^GATCA
HaeII 1639 AGCGCT [GA]GCGC^[CT]
HgaI 2250 GACGC GACGC(5/10)
MaeII 1316 ACGT A^CGT
MfeI 224 CAATTG C^AATTG
MflI 104 GGATCC [GA]^GATC[CT]
MunI 224 CAATTG C^AATTG
NdeI 1184 CATATG CA^TATG
NheI 841 GCTAGC G^CTAGC
PflFI 1090 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 1315 AACGTT AA^CGTT
PstI 153 CTGCAG CTGCA^G
ScaI 2341 AGTACT AGT^ACT
SmlI 1044 CTCAAG C^T[CT][GA]AG
SpeI 538 ACTAGT A^CTAGT
SspI 1513 AATATT AAT^ATT
TaqI 1939 TCGA T^CGA
Tth111I 1090 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1939 TCGA T^CGA
XcmI 272 CCAGAATCCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 104 GGATCC [GA]^GATC[CT]
XmnI 581 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 548 CGCG CG^CG
2252 CGCG
Alw44I 1325 GTGCAC G^TGCAC
1575 GTGCAC
ApaLI 1325 GTGCAC G^TGCAC
1575 GTGCAC
AseI 1260 ATTAAT AT^TAAT
1713 ATTAAT
AsnI 1260 ATTAAT AT^TAAT
1713 ATTAAT
AspHI 1325 GTGCAC G[AT]GC[AT]^C
1575 GTGCAC
AvaII 295 GGACC G^G[AT]CC
1670 GGACC
BbsI 2098 GTCTTC GAAGAC(2/6)
2176 GTCTTC
BcgI 1940 CGATCAAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2087 GCATCAGGATCG
BpuAI 2098 GTCTTC GAAGAC(2/6)
2176 GTCTTC
BsaBI 93 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
99 GATCAGGATC
BsiHKAI 1325 GTGCAC G[AT]GC[AT]^C
1575 GTGCAC
BsmAI 715 GAGAC GTCTC(1/5)
365 GTCTC
BstUI 548 CGCG CG^CG
2252 CGCG
CfoI 1224 GCGC GCG^C
1640 GCGC
HapII 354 CCGG C^CGG
1809 CCGG
HhaI 1224 GCGC GCG^C
1640 GCGC
HinP1I 1224 GCGC G^CGC
1640 GCGC
HincII 626 GTCAAC GT[CT]^[GA]AC
1087 GTTGAC
HindII 626 GTCAAC GT[CT]^[GA]AC
1087 GTTGAC
HindIII 988 AAGCTT A^AGCTT
2189 AAGCTT
HpaII 354 CCGG C^CGG
1809 CCGG
MamI 93 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
99 GATCAGGATC
MslI 877 CATACAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1425 CATATCAATG
MspA1I 1230 CCGCTG C[AC]G^C[GT]G
1867 CCGCTG
MspI 354 CCGG C^CGG
1809 CCGG
MvnI 548 CGCG CG^CG
2252 CGCG
NspI 310 ACATGT [GA]CATG^[CT]
958 ACATGC
PleI 624 GAGTC GAGTC(4/5)
1727 GAGTC
PshBI 1260 ATTAAT AT^TAAT
1713 ATTAAT
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApoI BbvI BfaI BmyI
BsaJI BsgI BsmFI Bsp1286I Bsp1407I
BsrDI BsrGI BsrI BssKI BstF5I
BstNI Bsu36I Cac8I Cfr13I DdeI
DpnII EarI Eco81I EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspBI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI