Restriction map for
pFN21AE4081
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
CelII CfoI ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeII
MamI MfeI MflI MluI MroI
MspA1I MunI MvnI MwoI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI SwaI Tth111I XbaI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AflIII 561 ACATGT A^C[GA][CT]GT
AlwNI 18 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 762 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 504 CTCGGG C^[CT]CG[GA]G
AvaII 747 GGACC G^G[AT]CC
AvrII 352 CCTAGG C^CTAGG
BcnI 1071 CCGGG CC^[GC]GG
BlnI 352 CCTAGG C^CTAGG
BseRI 1133 CTCCTC GAGGAG(10/8)
BsmFI 81 GGGAC GGGAC(10/14)
BsoBI 504 CTCGGG C^[CT]CG[GA]G
BspHI 1269 TCATGA T^CATGA
BspLU11I 561 ACATGT A^CATGT
BsrFI 1004 ACCGGC [GA]^CCGG[CT]
BstXI 728 CCAGTGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1004 ACCGGC [GA]^CCGG[CT]
Cfr13I 747 GGACC G^G[ACGT]CC
DpnII 1278 GATC ^GATC
EcoRI 230 GAATTC G^AATTC
EcoRV 357 GATATC GAT^ATC
FauI 45 GCGGG CCCGC(4/6)
MboI 1278 GATC ^GATC
MslI 1201 CATCATAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 1071 CCGGG CC^[GC]GG
NdeII 1278 GATC ^GATC
NspI 561 ACATGT [GA]CATG^[CT]
PflMI 831 CCAAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1269 TCATGA T^CATGA
Sau3AI 1278 GATC ^GATC
Sau96I 747 GGACC G^G[ACGT]CC
SfcI 1215 CTATAG C^T[GA][CT]AG
SpeI 945 ACTAGT A^CTAGT
SspI 276 AATATT AAT^ATT
StuI 543 AGGCCT AGG^CCT
TaqI 719 TCGA T^CGA
TthHB8I 719 TCGA T^CGA
Van91I 831 CCAAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 203 CCATCTTTGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 762 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 230 GAATTC [GA]^AATT[CT]
937 GAATTT
ApoI 230 GAATTC [GA]^AATT[CT]
937 GAATTT
BalI 14 TGGCCA TGG^CCA
921 TGGCCA
BstF5I 349 CATCC GGATG(2/0)
600 CATCC
BstNI 906 CCAGG CC^[AT]GG
1236 CCTGG
Cac8I 624 GCGTGC GC[ACGT]^[ACGT]GC
708 GCAGGC
EarI 301 GAAGAG CTCTTC(1/4)
313 GAAGAG
EcoRII 906 CCAGG ^CC[AT]GG
1236 CCTGG
EcoT14I 123 CCAAGG C^C[AT][AT]GG
352 CCTAGG
FokI 349 CATCC GGATG(9/13)
600 CATCC
HapII 1005 CCGG C^CGG
1071 CCGG
HpaII 1005 CCGG C^CGG
1071 CCGG
Ksp632I 301 GAAGAG CTCTTC(1/4)
313 GAAGAG
MluNI 14 TGGCCA TGG^CCA
921 TGGCCA
MscI 14 TGGCCA TGG^CCA
921 TGGCCA
MspI 1005 CCGG C^CGG
1071 CCGG
MvaI 906 CCAGG CC^[AT]GG
1236 CCTGG
PleI 66 GACTC GAGTC(4/5)
1110 GAGTC
SmlI 631 CTTGAG C^T[CT][GA]AG
1180 CTTGAG
StyI 123 CCAAGG C^C[AT][AT]GG
352 CCTAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AluI AspHI BbvI BfaI
BmyI BsaJI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI DdeI
EaeI Fnu4HI HaeIII HinfI HphI
ItaI MaeI MaeIII MboII MnlI
MseI NlaIII NlaIV ScrFI SfaNI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI