Restriction map for
pFN21AE2272
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# NO CUT ENZYMES ( 187 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BcnI BfaI BfrI BglI BglII
BlnI BlpI BmyI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsiYI
BslI BsmAI BsmBI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
Cfr13I ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT14I EcoT22I FauI
FseI FspI HaeII HapII HhaI
HinP1I HpaII HphI KasI KpnI
KspI MaeI MaeII MamI MfeI
MluI MroI MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI Sau96I SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tsp45I TspRI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AcyI 3 GGCGTC G[GA]^CG[CT]C
AfaI 68 GTAC GT^AC
BamHI 348 GGATCC G^GATCC
BbsI 213 GAAGAC GAAGAC(2/6)
BclI 422 TGATCA T^GATCA
BpmI 296 CTGGAG CTGGAG(16/14)
BpuAI 213 GAAGAC GAAGAC(2/6)
BsaHI 3 GGCGTC G[GA]^CG[CT]C
BsaJI 26 CCGTGG C^C[ACGT][ACGT]GG
BsmFI 6 GTCCC GGGAC(10/14)
BsrDI 301 GCAATG GCAATG(2/0)
BssKI 477 CCAGG ^CC[ACGT]GG
BstF5I 30 GGATG GGATG(2/0)
BstNI 477 CCAGG CC^[AT]GG
BstYI 348 GGATCC [GA]^GATC[CT]
DdeI 594 CTCAG C^T[ACGT]AG
DsaI 26 CCGTGG C^C[GA][CT]GG
EcoRI 276 GAATTC G^AATTC
EcoRII 477 CCAGG ^CC[AT]GG
FbaI 422 TGATCA T^GATCA
FokI 30 GGATG GGATG(9/13)
HgaI 4 GCGTC GACGC(5/10)
Hin1I 3 GGCGTC G[GA]^CG[CT]C
HincII 334 GTTAAC GT[CT]^[GA]AC
HindII 334 GTTAAC GT[CT]^[GA]AC
HpaI 334 GTTAAC GTT^AAC
MflI 348 GGATCC [GA]^GATC[CT]
MslI 396 CATGAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 477 CCAGG CC^[AT]GG
MwoI 541 GCTCCATCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII 396 CATG CATG^
NlaIV 348 GGATCC GG[ACGT]^[ACGT]CC
RsaI 68 GTAC GT^AC
ScrFI 477 CCAGG CC^[ACGT]GG
SfaNI 198 GCATC GCATC(5/9)
SmlI 619 CTCAAG C^T[CT][GA]AG
XhoII 348 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 255 GTCTAC GT^[AC][GT]AC
306 GTCTAC
AlwI 349 GATCC GGATC(4/5)
348 GGATC
BalI 146 TGGCCA TGG^CCA
533 TGGCCA
BbvI 97 GCAGC GCAGC(8/12)
550 GCTGC
Cac8I 169 GCTTGC GC[ACGT]^[ACGT]GC
327 GCAGGC
EaeI 146 TGGCCA [CT]^GGCC[GA]
533 TGGCCA
EarI 10 CTCTTC CTCTTC(1/4)
601 GAAGAG
Fnu4HI 97 GCAGC GC^[ACGT]GC
550 GCTGC
HaeIII 147 GGCC GG^CC
534 GGCC
HindIII 167 AAGCTT A^AGCTT
558 AAGCTT
HinfI 51 GAATC G^A[ACGT]TC
489 GAATC
ItaI 97 GCAGC GC^[ACGT]GC
550 GCTGC
Ksp632I 10 CTCTTC CTCTTC(1/4)
601 GAAGAG
MaeIII 60 GTTAC ^GT[ACGT]AC
244 GTTAC
MluNI 146 TGGCCA TGG^CCA
533 TGGCCA
MscI 146 TGGCCA TGG^CCA
533 TGGCCA
MseI 137 TTAA T^TAA
335 TTAA
MspA1I 121 CAGCTG C[AC]G^C[GT]G
548 CAGCTG
PvuII 121 CAGCTG CAG^CTG
548 CAGCTG
TfiI 51 GAATC G^A[AT]TC
489 GAATC
Tru9I 137 TTAA T^TAA
335 TTAA
TseI 97 GCAGC G^C[AT]GC
550 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
#########################################
AcsI AluI ApoI DpnII MboI
MboII MnlI NdeII Sau3AI TaqI
Tsp509I TthHB8I