##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AviII       AvrII       BamHI       
BbrPI       BbsI        BciVI       BclI        BfrI        
BglII       BlnI        BpuAI       BsaAI       BsaBI       
BsaI        BsaWI       BseAI       BsiHKAI     BsiWI       
BsmAI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrGI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstXI       BstYI       BstZ17I     Bsu36I      
ClaI        CpoI        DraI        DraIII      DrdI        
Eam1105I    Eco47III    Eco81I      EcoNI       EcoO65I     
EcoRV       EcoT14I     EcoT22I     FbaI        FseI        
FspI        HincII      HindII      HindIII     HinfI       
HpaI        KpnI        MamI        MfeI        MflI        
MluI        MroI        MseI        MunI        NcoI        
NdeI        NheI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SalI        
SbfI        ScaI        SexAI       SfcI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StyI        SwaI        TfiI        
Tru9I       Tth111I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AccI          598  GTCTAC               GT^[AC][GT]AC
AcsI          823  GAATTC               [GA]^AATT[CT]
AfaI          159  GTAC                 GT^AC
ApoI          823  GAATTC               [GA]^AATT[CT]
AvaI          693  CCCGGG               C^[CT]CG[GA]G
BbvCI        1032  GCTGAGG              CCTCAGC(-5/-2)
BcgI          284  GCAAGCTGCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI           97  CTAG                 C^TAG
BlpI           72  GCTCAGC              GC^T[ACGT]AGC
BpmI          517  CTGGAG               CTGGAG(16/14)
Bpu1102I       72  GCTCAGC              GC^T[ACGT]AGC
BsgI          145  CTGCAC               GTGCAG(16/14)
BsoBI         693  CCCGGG               C^[CT]CG[GA]G
BssSI         811  CTCGTG               CACGAG(-5/-1)
CelII          72  GCTCAGC              GC^T[ACGT]AGC
DpnII         126  GATC                 ^GATC
EcoRI         823  GAATTC               G^AATTC
HphI          353  TCACC                GGTGA(8/7)
MaeI           97  CTAG                 C^TAG
MboI          126  GATC                 ^GATC
MslI          423  CATGCTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII         126  GATC                 ^GATC
PpuMI         920  GGGTCCT              [GA]G^G[AT]CC[CT]
RsaI          159  GTAC                 GT^AC
Sau3AI        126  GATC                 ^GATC
SfiI          776  GGCCCGCAGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI          693  CCCGGG               CCC^GGG
SmlI           58  CTCAAG               C^T[CT][GA]AG
StuI          950  AGGCCT               AGG^CCT
TaqI          173  TCGA                 T^CGA
Tsp509I       824  AATT                 ^AATT
TspRI         473  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       173  TCGA                 T^CGA
XmaCI         693  CCCGGG               C^CCGGG
XmaI          693  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

BseRI         808  CTCCTC               GAGGAG(10/8)
              883  CTCCTC              
BsmFI        1037  GGGAC                GGGAC(10/14)
              943  GTCCC               
BspMI         164  ACCTGC               ACCTGC(4/8)
              914  ACCTGC              
DdeI           73  CTCAG                C^T[ACGT]AG
             1033  CTGAG               
HgaI          502  GACGC                GACGC(5/10)
              350  GCGTC               
MaeII         107  ACGT                 A^CGT
              899  ACGT                
MaeIII        352  GTCAC                ^GT[ACGT]AC
              865  GTCAC               
NaeI          403  GCCGGC               GCC^GGC
              991  GCCGGC              
NgoMIV        403  GCCGGC               G^CCGGC
              991  GCCGGC              
NlaIII        225  CATG                 CATG^
              423  CATG                
Tsp45I        352  GTCAC                ^GT[GC]AC
              865  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   74 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        ApaI        
AvaII       BalI        BanI        BanII       BbeI        
BbvI        BcnI        BglI        BmyI        BsaHI       
BsaJI       BsiEI       BsiYI       BslI        Bsp1286I    
BsrFI       BsrI        BssHII      BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr10I      
Cfr13I      DraII       DsaI        EaeI        EagI        
EarI        EclXI       Eco52I      EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        Hin1I       HinP1I      HpaII       
ItaI        KasI        Ksp632I     KspI        MboII       
MluNI       MnlI        MscI        MspA1I      MspI        
MvaI        MvnI        MwoI        NarI        NciI        
NlaIV       NotI        SacII       SapI        Sau96I      
ScrFI       SfaNI       SfoI        TseI