Restriction map for
pFN21AE6568
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# NO CUT ENZYMES ( 173 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaII
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BciVI BclI BfrI BglI BglII
BlnI BlpI BmyI Bpu1102I BpuAI
BsaAI BsaI BsaWI BseAI BsgI
BsiHKAI BsiWI BsmBI BsmI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstYI BstZ17I Bsu36I CelII
Cfr10I Cfr13I ClaI CpoI DraI
DraII DraIII DrdI DsaI Eam1105I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I FauI FbaI FseI
FspI HgaI HincII HindII HindIII
HpaI HphI KasI KpnI KspI
MaeIII MfeI MflI MluI MluNI
MroI MscI MslI MspA1I MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI Sau96I SbfI ScaI SfcI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI Tsp45I
Tsp509I Tth111I TthHB8I Van91I XbaI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 262 GACGTC GACGT^C
AcyI 262 GACGTC G[GA]^CG[CT]C
AluI 325 AGCT AG^CT
AlwI 198 GATCC GGATC(4/5)
Aor51HI 284 AGCGCT AGC^GCT
AvaI 45 CCCGGG C^[CT]CG[GA]G
BcgI 54 CGATCGCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 505 CTAG C^TAG
BpmI 442 CTGGAG CTGGAG(16/14)
BsaBI 396 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 262 GACGTC G[GA]^CG[CT]C
BseRI 350 GAGGAG GAGGAG(10/8)
BsmAI 417 GAGAC GTCTC(1/5)
BsoBI 45 CCCGGG C^[CT]CG[GA]G
BspMI 116 ACCTGC ACCTGC(4/8)
BsrI 441 ACTGG ACTGG(1/-1)
BstXI 134 CCAACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 322 GCGAGC GC[ACGT]^[ACGT]GC
DdeI 181 CTGAG C^T[ACGT]AG
EaeI 67 CGGCCG [CT]^GGCC[GA]
EagI 67 CGGCCG C^GGCCG
EarI 471 GAAGAG CTCTTC(1/4)
EclXI 67 CGGCCG C^GGCCG
Eco47III 284 AGCGCT AGC^GCT
Eco52I 67 CGGCCG C^GGCCG
EcoT22I 407 ATGCAT ATGCA^T
HaeII 284 AGCGCT [GA]GCGC^[CT]
HaeIII 68 GGCC GG^CC
Hin1I 262 GACGTC G[GA]^CG[CT]C
Ksp632I 471 GAAGAG CTCTTC(1/4)
MaeI 505 CTAG C^TAG
MamI 396 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 8 TTAA T^TAA
MwoI 104 GCGTAGTGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NsiI 407 ATGCAT ATGCA^T
PvuI 54 CGATCG CGAT^CG
SexAI 140 ACCTGGT A^CC[AT]GGT
SmaI 45 CCCGGG CCC^GGG
SmlI 430 CTCAAG C^T[CT][GA]AG
TfiI 209 GAATC G^A[AT]TC
Tru9I 8 TTAA T^TAA
TspRI 537 CACTG CA[GC]TG[ACGT][ACGT]^
XmaCI 45 CCCGGG C^CCGGG
XmaI 45 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccII 25 CGCG CG^CG
103 CGCG
AciI 24 CCGC CCGC(-3/-1)
102 CCGC
BbvI 247 GCAGC GCAGC(8/12)
151 GCTGC
BsiEI 54 CGATCG CG[GA][CT]^CG
67 CGGCCG
BstF5I 275 GGATG GGATG(2/0)
498 GGATG
BstUI 25 CGCG CG^CG
103 CGCG
CfoI 26 GCGC GCG^C
285 GCGC
DpnII 55 GATC ^GATC
198 GATC
Fnu4HI 151 GCTGC GC^[ACGT]GC
247 GCAGC
FokI 275 GGATG GGATG(9/13)
498 GGATG
HhaI 26 GCGC GCG^C
285 GCGC
HinP1I 26 GCGC G^CGC
285 GCGC
ItaI 151 GCTGC GC^[ACGT]GC
247 GCAGC
MboI 55 GATC ^GATC
198 GATC
MvnI 25 CGCG CG^CG
103 CGCG
NdeII 55 GATC ^GATC
198 GATC
NlaIV 49 GGTTCC GG[ACGT]^[ACGT]CC
425 GGAACC
Sau3AI 55 GATC ^GATC
198 GATC
TseI 151 GCTGC G^C[AT]GC
247 GCAGC
XcmI 19 CCACTCCGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
134 CCAACTACCTGGTGG
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# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
BcnI BsaJI BsiYI BslI BsmFI
BssKI BstNI EcoRII HapII HinfI
HpaII MaeII MboII MnlI MspI
MvaI NciI NlaIII PleI ScrFI
SfaNI