Restriction map for
pFN21AE5248
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# NO CUT ENZYMES ( 187 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BbvI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BpuAI BsaAI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI Fnu4HI FseI FspI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII HphI ItaI KasI KpnI
KspI MfeI MluI MluNI MroI
MscI MslI MspA1I MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI TseI TspRI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AlwI 601 GATCC GGATC(4/5)
AseI 79 ATTAAT AT^TAAT
AsnI 79 ATTAAT AT^TAAT
Asp700I 93 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 122 GGTCC G^G[AT]CC
BlpI 159 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 159 GCTGAGC GC^T[ACGT]AGC
BsaBI 214 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 125 CCCAGG C^C[ACGT][ACGT]GG
BseRI 40 CTCCTC GAGGAG(10/8)
BsiYI 126 CCAGGAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 126 CCAGGAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 123 GTCCC GGGAC(10/14)
BsrI 144 CCAGT ACTGG(1/-1)
BssKI 126 CCAGG ^CC[ACGT]GG
BstNI 126 CCAGG CC^[AT]GG
BstYI 600 AGATCC [GA]^GATC[CT]
CelII 159 GCTGAGC GC^T[ACGT]AGC
Cfr13I 122 GGTCC G^G[ACGT]CC
DraII 121 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 121 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRII 126 CCAGG ^CC[AT]GG
HgaI 36 GCGTC GACGC(5/10)
MaeII 153 ACGT A^CGT
MaeIII 10 GTGAC ^GT[ACGT]AC
MamI 214 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 600 AGATCC [GA]^GATC[CT]
MvaI 126 CCAGG CC^[AT]GG
PpuMI 121 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 79 ATTAAT AT^TAAT
Sau96I 122 GGTCC G^G[ACGT]CC
ScrFI 126 CCAGG CC^[ACGT]GG
SpeI 204 ACTAGT A^CTAGT
Tsp45I 10 GTGAC ^GT[GC]AC
XhoII 600 AGATCC [GA]^GATC[CT]
XmnI 93 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 147 GTAC GT^AC
515 GTAC
AspHI 162 GAGCAC G[AT]GC[AT]^C
281 GAGCAC
BmyI 162 GAGCAC G[AGT]GC[ACT]^C
281 GAGCAC
BpmI 62 CTGGAG CTGGAG(16/14)
278 CTGGAG
BsiHKAI 162 GAGCAC G[AT]GC[AT]^C
281 GAGCAC
BsmBI 37 CGTCTC CGTCTC(1/5)
486 GAGACG
Bsp1286I 162 GAGCAC G[AGT]GC[ACT]^C
281 GAGCAC
BstF5I 385 CATCC GGATG(2/0)
519 CATCC
Cac8I 164 GCACGC GC[ACGT]^[ACGT]GC
586 GCAAGC
DpnII 70 GATC ^GATC
601 GATC
EarI 93 GAAGAG CTCTTC(1/4)
130 GAAGAG
FokI 385 CATCC GGATG(9/13)
519 CATCC
HinfI 219 GAATC G^A[ACGT]TC
375 GATTC
Ksp632I 93 GAAGAG CTCTTC(1/4)
130 GAAGAG
MboI 70 GATC ^GATC
601 GATC
MwoI 159 GCTGAGCACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
577 GCTCAGGAAGC
NdeII 70 GATC ^GATC
601 GATC
RsaI 147 GTAC GT^AC
515 GTAC
Sau3AI 70 GATC ^GATC
601 GATC
SfaNI 358 GATGC GCATC(5/9)
441 GCATC
TfiI 219 GAATC G^A[AT]TC
375 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AcsI AluI ApoI BfaI BsmAI
DdeI MaeI MboII MnlI MseI
NlaIII NlaIV Tru9I Tsp509I