Restriction map for
pFN21AE1227
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# NO CUT ENZYMES ( 167 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BciVI BclI
BcnI BfaI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BseAI BseRI BsgI BsiEI
BsiWI BsiYI BslI BsmFI BspDI
BspEI BspLU11I BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
FauI FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HpaI KasI
KpnI Ksp632I KspI MaeI MaeII
MamI MboII MfeI MluI MluNI
MroI MscI MslI MspA1I MunI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI StyI SwaI
TfiI Tth111I Van91I XbaI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AcsI 601 AAATTC [GA]^AATT[CT]
AfaI 177 GTAC GT^AC
ApoI 601 AAATTC [GA]^AATT[CT]
AspHI 136 GTGCTC G[AT]GC[AT]^C
AvaI 111 CTCGAG C^[CT]CG[GA]G
AvaII 198 GGACC G^G[AT]CC
BcgI 567 GCAGTATGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI 136 GTGCTC G[AGT]GC[ACT]^C
BsaJI 423 CCAGGG C^C[ACGT][ACGT]GG
BsaWI 407 TCCGGT [AT]^CCGG[AT]
BsiHKAI 136 GTGCTC G[AT]GC[AT]^C
BsmBI 100 GAGACG CGTCTC(1/5)
BsmI 667 GCATTC GAATGC(1/-1)
BsoBI 111 CTCGAG C^[CT]CG[GA]G
Bsp1286I 136 GTGCTC G[AGT]GC[ACT]^C
Bsp1407I 176 TGTACA T^GTACA
BspHI 881 TCATGA T^CATGA
BspMI 490 GCAGGT ACCTGC(4/8)
BsrBI 807 GAGCGG CCGCTC(-3/-3)
BsrDI 608 GCAATG GCAATG(2/0)
BsrGI 176 TGTACA T^GTACA
Cac8I 617 GCAAGC GC[ACGT]^[ACGT]GC
DraII 425 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 425 AGGGCCT [GA]G^G[ACGT]CC[CT]
HapII 408 CCGG C^CGG
HgaI 35 GCGTC GACGC(5/10)
HincII 496 GTCAAC GT[CT]^[GA]AC
HindII 496 GTCAAC GT[CT]^[GA]AC
HindIII 514 AAGCTT A^AGCTT
HpaII 408 CCGG C^CGG
MspI 408 CCGG C^CGG
NlaIV 543 GGGGCC GG[ACGT]^[ACGT]CC
PaeR7I 111 CTCGAG C^TCGAG
RcaI 881 TCATGA T^CATGA
RsaI 177 GTAC GT^AC
SmlI 111 CTCGAG C^T[CT][GA]AG
SspBI 176 TGTACA T^GTACA
XhoI 111 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AccII 34 CGCG CG^CG
52 CGCG
BglI 89 GCCCCAACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
149 GCCAGGAGGGC
BpmI 743 CTCCAG CTGGAG(16/14)
727 CTGGAG
BsaI 374 GGTCTC GGTCTC(1/5)
410 GGTCTC
BstUI 34 CGCG CG^CG
52 CGCG
BstYI 402 GGATCT [GA]^GATC[CT]
785 AGATCC
DdeI 41 CTAAG C^T[ACGT]AG
752 CTCAG
EcoT22I 433 ATGCAT ATGCA^T
665 ATGCAT
HphI 681 GGTGA GGTGA(8/7)
346 TCACC
MflI 402 GGATCT [GA]^GATC[CT]
785 AGATCC
MvnI 34 CGCG CG^CG
52 CGCG
NsiI 433 ATGCAT ATGCA^T
665 ATGCAT
PstI 439 CTGCAG CTGCA^G
488 CTGCAG
SfcI 439 CTGCAG C^T[GA][CT]AG
488 CTGCAG
Tsp45I 345 GTCAC ^GT[GC]AC
721 GTCAC
TspRI 725 CACTG CA[GC]TG[ACGT][ACGT]^
894 CAGTG
XcmI 341 CCATGTCACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
789 CCATGTCTGGAATGG
XhoII 402 GGATCT [GA]^GATC[CT]
785 AGATCC
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AluI AlwI BbvI BsmAI
BsrI BssKI BstF5I BstNI Cfr13I
DpnII EcoRII Fnu4HI FokI HaeIII
HinfI ItaI MaeIII MboI MnlI
MseI MvaI MwoI NdeII NlaIII
PleI Sau3AI Sau96I ScrFI SfaNI
TaqI Tru9I TseI Tsp509I TthHB8I