Restriction map for
pFN21AE3348
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# NO CUT ENZYMES ( 73 enzymes) #
##################################
AccI AclI AcsI AflII AflIII
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I AvrII BamHI
BbrPI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BsiWI
BspDI BspLU11I Bst1107I BstBI BstZ17I
CelII ClaI DraI DrdI Eco47III
EcoRI EcoRV FbaI FseI HincII
HindII HpaI KspI MamI MfeI
MluI MunI NheI NotI NruI
NspV PacI PaeR7I PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
SacII SalI ScaI SfiI SfuI
SgrAI SnaBI SpeI SspI SwaI
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AatII 2890 GACGTC GACGT^C
Acc65I 79 GGTACC G^GTACC
AccIII 2108 TCCGGA T^CCGGA
AgeI 1561 ACCGGT A^CCGGT
AhdI 1182 GACTCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 709 CCTGAGG CC^T[ACGT]AGG
Asp718I 79 GGTACC G^GTACC
AspEI 1182 GACTCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2821 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 573 CCCGGG C^[CT]CG[GA]G
BbeI 705 GGCGCC GGCGC^C
BseAI 2108 TCCGGA T^CCGGA
BsmBI 1975 CGTCTC CGTCTC(1/5)
BsmI 897 GAATGC GAATGC(1/-1)
BsoBI 573 CCCGGG C^[CT]CG[GA]G
Bsp1407I 2660 TGTACA T^GTACA
BspEI 2108 TCCGGA T^CCGGA
BspHI 404 TCATGA T^CATGA
BsrGI 2660 TGTACA T^GTACA
BssHII 2841 GCGCGC G^CGCGC
BssSI 1609 CACGAG CACGAG(-5/-1)
BstEII 1452 GGTGACC G^GT[ACGT]ACC
BstPI 1452 GGTGACC G^GT[ACGT]ACC
BstYI 1431 GGATCT [GA]^GATC[CT]
Bsu36I 709 CCTGAGG CC^T[ACGT]AGG
CpoI 2819 CGGACCG CG^G[AT]CCG
DraIII 179 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1182 GACTCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 376 GAAGAG CTCTTC(1/4)
Eco81I 709 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1452 GGTGACC G^GT[ACGT]ACC
EcoT22I 966 ATGCAT ATGCA^T
HindIII 1012 AAGCTT A^AGCTT
KasI 705 GGCGCC G^GCGCC
KpnI 79 GGTACC GGTAC^C
Ksp632I 376 GAAGAG CTCTTC(1/4)
MflI 1431 GGATCT [GA]^GATC[CT]
MroI 2108 TCCGGA T^CCGGA
NaeI 490 GCCGGC GCC^GGC
NarI 705 GGCGCC GG^CGCC
NdeI 1834 CATATG CA^TATG
NgoMIV 490 GCCGGC G^CCGGC
NsiI 966 ATGCAT ATGCA^T
PflFI 2821 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 117 CCACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1561 ACCGGT A^CCGGT
RcaI 404 TCATGA T^CATGA
RsrII 2819 CGGACCG CG^G[AT]CCG
SapI 376 GAAGAGC GCTCTTC(1/4)
SbfI 548 CCTGCAGG CCTGCA^GG
SexAI 1448 ACCTGGT A^CC[AT]GGT
SfoI 705 GGCGCC GGC^GCC
SmaI 573 CCCGGG CCC^GGG
Sse8387I 548 CCTGCAGG CCTGCA^GG
SspBI 2660 TGTACA T^GTACA
TfiI 238 GATTC G^A[AT]TC
Tth111I 2821 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 117 CCACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1431 GGATCT [GA]^GATC[CT]
XmaCI 573 CCCGGG C^CCGGG
XmaI 573 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 2731 CGCG CG^CG
2842 CGCG
AcyI 705 GGCGCC G[GA]^CG[CT]C
2890 GACGTC
AlwI 2907 GATCC GGATC(4/5)
1431 GGATC
BalI 59 TGGCCA TGG^CCA
719 TGGCCA
BbsI 1390 GAAGAC GAAGAC(2/6)
2623 GTCTTC
BpuAI 1390 GAAGAC GAAGAC(2/6)
2623 GTCTTC
BsaAI 1255 TACGTG [CT]AC^GT[GA]
2938 TACGTG
BsaHI 705 GGCGCC G[GA]^CG[CT]C
2890 GACGTC
BseRI 2977 CTCCTC GAGGAG(10/8)
2686 GAGGAG
BsiEI 2922 CGGCCG CG[GA][CT]^CG
3089 CGGCCG
BsrDI 188 GCAATG GCAATG(2/0)
1820 GCAATG
BstUI 2731 CGCG CG^CG
2842 CGCG
EagI 2922 CGGCCG C^GGCCG
3089 CGGCCG
EclXI 2922 CGGCCG C^GGCCG
3089 CGGCCG
Eco52I 2922 CGGCCG C^GGCCG
3089 CGGCCG
Hin1I 705 GGCGCC G[GA]^CG[CT]C
2890 GACGTC
MluNI 59 TGGCCA TGG^CCA
719 TGGCCA
MscI 59 TGGCCA TGG^CCA
719 TGGCCA
MseI 1016 TTAA T^TAA
2003 TTAA
MvnI 2731 CGCG CG^CG
2842 CGCG
NcoI 170 CCATGG C^CATGG
1307 CCATGG
PleI 475 GACTC GAGTC(4/5)
1182 GACTC
SacI 106 GAGCTC GAGCT^C
2185 GAGCTC
SphI 498 GCATGC GCATG^C
1679 GCATGC
Tru9I 1016 TTAA T^TAA
2003 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AfaI AluI AlwNI ApaI
AspHI AvaII AviII BanI BanII
BbvCI BbvI BcgI BcnI BfaI
BglI BmyI BpmI BsaI BsaJI
BsaWI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstXI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI