Restriction map for
pFN21AE2792
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbeI BbrPI BbvCI
BciVI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmFI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI HphI KasI KpnI KspI
MaeI MaeII MamI MfeI MflI
MluI MroI MseI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
SspBI SspI StuI SwaI TaqI
TfiI Tru9I Tsp45I Tsp509I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 22 CGCG CG^CG
ApaI 306 GGGCCC GGGCC^C
AspHI 508 GAGCAC G[AT]GC[AT]^C
AvaI 384 CCCGGG C^[CT]CG[GA]G
AvaII 483 GGACC G^G[AT]CC
BalI 194 TGGCCA TGG^CCA
BbsI 450 GAAGAC GAAGAC(2/6)
BcgI 114 GCATGGTATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 450 GAAGAC GAAGAC(2/6)
BsiHKAI 508 GAGCAC G[AT]GC[AT]^C
BsoBI 384 CCCGGG C^[CT]CG[GA]G
BspHI 545 TCATGA T^CATGA
BspMI 203 ACCTGC ACCTGC(4/8)
BstAPI 235 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 22 CGCG CG^CG
BstXI 583 CCAGAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 21 GCGC GCG^C
DdeI 334 CTGAG C^T[ACGT]AG
DsaI 488 CCATGG C^C[GA][CT]GG
EaeI 194 TGGCCA [CT]^GGCC[GA]
EarI 55 CTCTTC CTCTTC(1/4)
FauI 556 GCGGG CCCGC(4/6)
HhaI 21 GCGC GCG^C
HinP1I 21 GCGC G^CGC
HinfI 453 GACTC G^A[ACGT]TC
Ksp632I 55 CTCTTC CTCTTC(1/4)
MaeIII 569 GTTAC ^GT[ACGT]AC
MluNI 194 TGGCCA TGG^CCA
MscI 194 TGGCCA TGG^CCA
MslI 33 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 520 CAGCTG C[AC]G^C[GT]G
MvnI 22 CGCG CG^CG
NcoI 488 CCATGG C^CATGG
NspI 341 GCATGT [GA]CATG^[CT]
PleI 453 GACTC GAGTC(4/5)
PpuMI 482 AGGACCC [GA]G^G[AT]CC[CT]
PstI 616 CTGCAG CTGCA^G
PvuII 520 CAGCTG CAG^CTG
RcaI 545 TCATGA T^CATGA
SbfI 615 CCTGCAGG CCTGCA^GG
SfaNI 292 GATGC GCATC(5/9)
SfcI 616 CTGCAG C^T[GA][CT]AG
SmaI 384 CCCGGG CCC^GGG
Sse8387I 615 CCTGCAGG CCTGCA^GG
XmaCI 384 CCCGGG C^CCGGG
XmaI 384 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AciI 280 GCGG CCGC(-3/-1)
556 GCGG
AlwNI 145 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
236 CAGCCCCTG
BanI 28 GGCACC G^G[CT][GA]CC
560 GGCACC
BanII 306 GGGCCC G[GA]GC[CT]^C
622 GGGCTC
BclI 320 TGATCA T^GATCA
542 TGATCA
BpmI 455 CTCCAG CTGGAG(16/14)
625 CTCCAG
BsaI 136 GAGACC GGTCTC(1/5)
476 GAGACC
BseRI 129 CTCCTC GAGGAG(10/8)
586 GAGGAG
BsmAI 136 GAGAC GTCTC(1/5)
476 GAGAC
BsrI 397 CCAGT ACTGG(1/-1)
443 CCAGT
BstF5I 33 CATCC GGATG(2/0)
291 GGATG
DpnII 321 GATC ^GATC
543 GATC
DraIII 332 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
532 CACCGGGTG
FbaI 320 TGATCA T^GATCA
542 TGATCA
FokI 33 CATCC GGATG(9/13)
291 GGATG
MboI 321 GATC ^GATC
543 GATC
NdeII 321 GATC ^GATC
543 GATC
Sau3AI 321 GATC ^GATC
543 GATC
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# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AfaI AluI BbvI BcnI BmyI
BsaJI BsgI BsiYI BslI Bsp1286I
BssKI BstNI Cac8I Cfr13I DraII
EcoO109I EcoRII EcoT14I Fnu4HI HaeIII
HapII HpaII ItaI MboII MnlI
MspI MvaI MwoI NciI NlaIII
NlaIV RsaI Sau96I ScrFI StyI
TseI TspRI