Restriction map for
pFN21AE3160
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BclI BfrI BglI
BlnI BlpI Bpu1102I BsaBI BsaHI
BseAI BsgI BsiEI BsiWI BsmBI
BspDI BspEI BspLU11I BssHII BssSI
BstBI BstEII BstPI BstUI CelII
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV FbaI FseI HaeII
HgaI Hin1I HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TfiI
Tsp509I XbaI XcmI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 730 GTATAC GT^[AC][GT]AC
Alw44I 432 GTGCAC G^TGCAC
AlwI 939 GATCC GGATC(4/5)
AocI 422 CCTCAGG CC^T[ACGT]AGG
ApaLI 432 GTGCAC G^TGCAC
AspI 112 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 659 TGCGCA TGC^GCA
BanI 384 GGTGCC G^G[CT][GA]CC
BbsI 85 GAAGAC GAAGAC(2/6)
BciVI 984 GTATCC GTATCC(6/5)
BglII 1058 AGATCT A^GATCT
BpuAI 85 GAAGAC GAAGAC(2/6)
BsaAI 799 TACGTG [CT]AC^GT[GA]
BsaI 884 GAGACC GGTCTC(1/5)
BsaWI 498 TCCGGT [AT]^CCGG[AT]
BsmI 22 GAATGC GAATGC(1/-1)
Bsp1407I 125 TGTACA T^GTACA
BspHI 392 TCATGA T^CATGA
BsrBI 1099 GAGCGG CCGCTC(-3/-3)
BsrFI 428 GCCGGT [GA]^CCGG[CT]
BsrGI 125 TGTACA T^GTACA
Bst1107I 730 GTATAC GTA^TAC
BstXI 398 CCATCTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1058 AGATCT [GA]^GATC[CT]
BstZ17I 730 GTATAC GTA^TAC
Bsu36I 422 CCTCAGG CC^T[ACGT]AGG
Cfr10I 428 GCCGGT [GA]^CCGG[CT]
DrdI 699 GACACTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 359 CCATGG C^C[GA][CT]GG
Eco81I 422 CCTCAGG CC^T[ACGT]AGG
EcoNI 777 CCTGCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 24 ATGCAT ATGCA^T
FauI 993 GCGGG CCCGC(4/6)
FspI 659 TGCGCA TGC^GCA
HindIII 157 AAGCTT A^AGCTT
MaeII 800 ACGT A^CGT
MflI 1058 AGATCT [GA]^GATC[CT]
MseI 305 TTAA T^TAA
NcoI 359 CCATGG C^CATGG
NsiI 24 ATGCAT ATGCA^T
PaeR7I 292 CTCGAG C^TCGAG
PflFI 112 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 910 CAGCTG CAG^CTG
RcaI 392 TCATGA T^CATGA
SacI 646 GAGCTC GAGCT^C
SapI 1006 GAAGAGC GCTCTTC(1/4)
ScaI 719 AGTACT AGT^ACT
SmlI 292 CTCGAG C^T[CT][GA]AG
SspBI 125 TGTACA T^GTACA
Tru9I 305 TTAA T^TAA
Tth111I 112 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 292 CTCGAG C^TCGAG
XhoII 1058 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
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AspHI 432 GTGCAC G[AT]GC[AT]^C
646 GAGCTC
BcnI 834 CCGGG CC^[GC]GG
1025 CCCGG
BfaI 334 CTAG C^TAG
340 CTAG
BpmI 715 CTCCAG CTGGAG(16/14)
406 CTGGAG
BsiHKAI 432 GTGCAC G[AT]GC[AT]^C
646 GAGCTC
BsmAI 884 GAGAC GTCTC(1/5)
830 GTCTC
BspMI 283 ACCTGC ACCTGC(4/8)
677 ACCTGC
BsrDI 663 CATTGC GCAATG(2/0)
233 GCAATG
BstAPI 5 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
17 GCATCGAATGC
CfoI 287 GCGC GCG^C
660 GCGC
DdeI 423 CTCAG C^T[ACGT]AG
862 CTGAG
EaeI 487 CGGCCA [CT]^GGCC[GA]
894 TGGCCG
EarI 244 CTCTTC CTCTTC(1/4)
1006 GAAGAG
EcoT14I 359 CCATGG C^C[AT][AT]GG
746 CCAAGG
HhaI 287 GCGC GCG^C
660 GCGC
HinP1I 287 GCGC G^CGC
660 GCGC
HinfI 451 GAGTC G^A[ACGT]TC
999 GACTC
Ksp632I 244 CTCTTC CTCTTC(1/4)
1006 GAAGAG
MaeI 334 CTAG C^TAG
340 CTAG
NciI 834 CCGGG CC^[GC]GG
1025 CCCGG
NspI 787 GCATGT [GA]CATG^[CT]
918 GCATGT
PflMI 753 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1086 CCATGAGCTGG
PleI 999 GACTC GAGTC(4/5)
451 GAGTC
SexAI 653 ACCTGGT A^CC[AT]GGT
758 ACCTGGT
StyI 359 CCATGG C^C[AT][AT]GG
746 CCAAGG
Van91I 753 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1086 CCATGAGCTGG
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaI
AvaII BanII BbvI BmyI BsaJI
BseRI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DpnII DraII EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HpaII HphI ItaI MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TseI
Tsp45I TspRI TthHB8I