Restriction map for
pFN21AE1930
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII Acc65I AclI AcyI AflII
AflIII AgeI AhdI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AvaI AviII BamHI BbeI
BbrPI BcgI BclI BfrI BglII
BlpI Bpu1102I BsaAI BsaHI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstYI
BstZ17I CelII ClaI CpoI DraI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HindIII
HpaI KasI KpnI KspI MfeI
MflI MluI MunI NarI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 52 CGCG CG^CG
AccIII 1166 TCCGGA T^CCGGA
Alw44I 18 GTGCAC G^TGCAC
AlwNI 887 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 18 GTGCAC G^TGCAC
AvaII 131 GGACC G^G[AT]CC
AvrII 1179 CCTAGG C^CTAGG
BanI 1147 GGTGCC G^G[CT][GA]CC
BbvCI 672 GCTGAGG CCTCAGC(-5/-2)
BcnI 980 CCCGG CC^[GC]GG
BglI 1194 GCCCAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1179 CCTAGG C^CTAGG
BsaBI 937 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 442 GAGACC GGTCTC(1/5)
BsaWI 1166 TCCGGA [AT]^CCGG[AT]
BseAI 1166 TCCGGA T^CCGGA
BsgI 1118 GTGCAG GTGCAG(16/14)
BsiEI 864 CGACCG CG[GA][CT]^CG
BsmI 1170 GAATGC GAATGC(1/-1)
BspEI 1166 TCCGGA T^CCGGA
BstAPI 20 GCACTATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 52 CGCG CG^CG
CfoI 528 GCGC GCG^C
DraII 1074 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 1100 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 295 GACAGCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 494 CCATGG C^C[GA][CT]GG
EcoO109I 1074 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 229 GAATTC G^AATTC
HaeII 527 AGCGCC [GA]GCGC^[CT]
HhaI 528 GCGC GCG^C
HinP1I 528 GCGC G^CGC
HincII 862 GTCGAC GT[CT]^[GA]AC
HindII 862 GTCGAC GT[CT]^[GA]AC
MaeII 56 ACGT A^CGT
MamI 937 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1166 TCCGGA T^CCGGA
MvnI 52 CGCG CG^CG
NciI 980 CCCGG CC^[GC]GG
NcoI 494 CCATGG C^CATGG
PvuII 586 CAGCTG CAG^CTG
SalI 862 GTCGAC G^TCGAC
SapI 183 GCTCTTC GCTCTTC(1/4)
ScaI 419 AGTACT AGT^ACT
SmlI 610 CTCAAG C^T[CT][GA]AG
Tsp45I 1032 GTCAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 715 GTCTAC GT^[AC][GT]AC
862 GTCGAC
AcsI 229 GAATTC [GA]^AATT[CT]
1273 AAATTC
AocI 532 CCTGAGG CC^T[ACGT]AGG
650 CCTCAGG
ApoI 229 GAATTC [GA]^AATT[CT]
1273 AAATTC
AspI 250 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1291 GACACAGTC
BalI 848 TGGCCA TGG^CCA
1107 TGGCCA
BbsI 489 GAAGAC GAAGAC(2/6)
1356 GAAGAC
BciVI 324 GGATAC GTATCC(6/5)
966 GGATAC
BfaI 1024 CTAG C^TAG
1180 CTAG
BpuAI 489 GAAGAC GAAGAC(2/6)
1356 GAAGAC
BspHI 791 TCATGA T^CATGA
1298 TCATGA
BspMI 133 ACCTGC ACCTGC(4/8)
836 ACCTGC
BsrDI 1310 CATTGC GCAATG(2/0)
341 GCAATG
BsrFI 470 GCCGGC [GA]^CCGG[CT]
917 GCCGGC
Bsu36I 532 CCTGAGG CC^T[ACGT]AGG
650 CCTCAGG
Cfr10I 470 GCCGGC [GA]^CCGG[CT]
917 GCCGGC
EarI 184 CTCTTC CTCTTC(1/4)
1372 CTCTTC
Eco81I 532 CCTGAGG CC^T[ACGT]AGG
650 CCTCAGG
Ksp632I 184 CTCTTC CTCTTC(1/4)
1372 CTCTTC
MaeI 1024 CTAG C^TAG
1180 CTAG
MaeIII 1004 GTTAC ^GT[ACGT]AC
1032 GTCAC
MluNI 848 TGGCCA TGG^CCA
1107 TGGCCA
MscI 848 TGGCCA TGG^CCA
1107 TGGCCA
MslI 558 CATGGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1293 CACAGTCATG
MspA1I 575 CAGCGG C[AC]G^C[GT]G
586 CAGCTG
NaeI 470 GCCGGC GCC^GGC
917 GCCGGC
NgoMIV 470 GCCGGC G^CCGGC
917 GCCGGC
NlaIV 1075 GGCCCC GG[ACGT]^[ACGT]CC
1147 GGTGCC
PflFI 250 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1291 GACACAGTC
PleI 1087 GAGTC GAGTC(4/5)
1213 GAGTC
PstI 361 CTGCAG CTGCA^G
820 CTGCAG
RcaI 791 TCATGA T^CATGA
1298 TCATGA
SfcI 361 CTGCAG C^T[GA][CT]AG
820 CTGCAG
StuI 368 AGGCCT AGG^CCT
647 AGGCCT
Tth111I 250 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1291 GACACAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
BanII BbvI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EaeI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MboI MboII MnlI
MseI MspI MvaI MwoI NdeII
NlaIII RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I