Restriction map for
pFN21AE6518
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BamHI BbeI BbvCI BciVI
BclI BcnI BfaI BfrI BglII
BlnI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiWI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HapII HgaI Hin1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MaeI MaeIII MfeI MflI
MluI MroI MseI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsaI RsrII SacII
SalI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp45I Tsp509I Tth111I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AocI 94 CCTAAGG CC^T[ACGT]AGG
BalI 470 TGGCCA TGG^CCA
BanI 382 GGCACC G^G[CT][GA]CC
BbrPI 463 CACGTG CAC^GTG
BcgI 105 CGAGTTTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 46 GCCCCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 451 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 451 GCTGAGC GC^T[ACGT]AGC
BsaAI 463 CACGTG [CT]AC^GT[GA]
BsaBI 204 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 183 CGACCG CG[GA][CT]^CG
BsrDI 431 GCAATG GCAATG(2/0)
BssKI 285 CCTGG ^CC[ACGT]GG
BstNI 285 CCTGG CC^[AT]GG
BstXI 317 CCAAGATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 94 CCTAAGG CC^T[ACGT]AGG
CelII 451 GCTGAGC GC^T[ACGT]AGC
EaeI 470 TGGCCA [CT]^GGCC[GA]
Eco81I 94 CCTAAGG CC^T[ACGT]AGG
EcoRII 285 CCTGG ^CC[AT]GG
HinfI 532 GACTC G^A[ACGT]TC
MaeII 464 ACGT A^CGT
MamI 204 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 470 TGGCCA TGG^CCA
MscI 470 TGGCCA TGG^CCA
MvaI 285 CCTGG CC^[AT]GG
PflMI 462 CCACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 532 GACTC GAGTC(4/5)
PmaCI 463 CACGTG CAC^GTG
PmlI 463 CACGTG CAC^GTG
SacI 307 GAGCTC GAGCT^C
SapI 304 GAAGAGC GCTCTTC(1/4)
ScrFI 285 CCTGG CC^[ACGT]GG
Van91I 462 CCACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AspHI 274 GTGCTC G[AT]GC[AT]^C
307 GAGCTC
BanII 19 GAGCCC G[GA]GC[CT]^C
307 GAGCTC
BbsI 496 GAAGAC GAAGAC(2/6)
415 GTCTTC
BpmI 59 CTCCAG CTGGAG(16/14)
352 CTCCAG
BpuAI 496 GAAGAC GAAGAC(2/6)
415 GTCTTC
BsiHKAI 274 GTGCTC G[AT]GC[AT]^C
307 GAGCTC
BstF5I 30 GGATG GGATG(2/0)
231 GGATG
Cac8I 240 GCGGGC GC[ACGT]^[ACGT]GC
468 GCTGGC
CfoI 90 GCGC GCG^C
244 GCGC
Cfr13I 3 GGCCC G^G[ACGT]CC
249 GGGCC
DpnII 123 GATC ^GATC
210 GATC
EarI 154 GAAGAG CTCTTC(1/4)
304 GAAGAG
EcoT14I 38 CCAAGG C^C[AT][AT]GG
50 CCAAGG
FokI 30 GGATG GGATG(9/13)
231 GGATG
HaeII 89 AGCGCC [GA]GCGC^[CT]
243 GGCGCT
HhaI 90 GCGC GCG^C
244 GCGC
HinP1I 90 GCGC G^CGC
244 GCGC
HphI 481 GGTGA GGTGA(8/7)
212 TCACC
Ksp632I 154 GAAGAG CTCTTC(1/4)
304 GAAGAG
MboI 123 GATC ^GATC
210 GATC
MslI 191 CACCCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
438 CACTGTCATG
NdeII 123 GATC ^GATC
210 GATC
NlaIV 3 GGCCCC GG[ACGT]^[ACGT]CC
382 GGCACC
Sau3AI 123 GATC ^GATC
210 GATC
Sau96I 3 GGCCC G^G[ACGT]CC
249 GGGCC
StyI 38 CCAAGG C^C[AT][AT]GG
50 CCAAGG
TspRI 223 CAGTG CA[GC]TG[ACGT][ACGT]^
438 CACTG
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# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
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AciI AluI BbvI BmyI BsaJI
BsiYI BslI Bsp1286I DdeI FauI
Fnu4HI HaeIII ItaI MboII MnlI
MwoI NlaIII SfaNI TaqI TseI
TthHB8I