Restriction map for
pFN21AE1699
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# NO CUT ENZYMES ( 174 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
AluI Alw44I AlwNI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BanI BbeI BbrPI BbsI
BbvCI BbvI BcgI BciVI BcnI
BfaI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoO65I EcoRV EcoT22I
Fnu4HI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaI HpaII
ItaI KasI KpnI Ksp632I KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MspA1I MspI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TaqI
TfiI TseI Tth111I TthHB8I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AciI 48 GCGG CCGC(-3/-1)
AcsI 362 GAATTC [GA]^AATT[CT]
AfaI 535 GTAC GT^AC
AflII 630 CTTAAG C^TTAAG
AocI 578 CCTCAGG CC^T[ACGT]AGG
ApaI 490 GGGCCC GGGCC^C
ApoI 362 GAATTC [GA]^AATT[CT]
AspHI 162 GTGCTC G[AT]GC[AT]^C
BamHI 338 GGATCC G^GATCC
BanII 490 GGGCCC G[GA]GC[CT]^C
BclI 416 TGATCA T^GATCA
BfrI 630 CTTAAG C^TTAAG
BpmI 222 CTGGAG CTGGAG(16/14)
BsaI 81 GAGACC GGTCTC(1/5)
BsiHKAI 162 GTGCTC G[AT]GC[AT]^C
BsmAI 81 GAGAC GTCTC(1/5)
BsmFI 8 GGGAC GGGAC(10/14)
BstAPI 635 GCATTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 125 GGATG GGATG(2/0)
BstYI 338 GGATCC [GA]^GATC[CT]
Bsu36I 578 CCTCAGG CC^T[ACGT]AGG
Cac8I 604 GCATGC GC[ACGT]^[ACGT]GC
DsaI 61 CCATGG C^C[GA][CT]GG
Eco81I 578 CCTCAGG CC^T[ACGT]AGG
EcoNI 146 CCTTCGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 362 GAATTC G^AATTC
EcoT14I 61 CCATGG C^C[AT][AT]GG
FauI 48 GCGGG CCCGC(4/6)
FbaI 416 TGATCA T^GATCA
FokI 125 GGATG GGATG(9/13)
MaeII 107 ACGT A^CGT
MflI 338 GGATCC [GA]^GATC[CT]
NcoI 61 CCATGG C^CATGG
PflMI 195 CCAAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 305 AGGACCC [GA]G^G[AT]CC[CT]
PstI 472 CTGCAG CTGCA^G
RsaI 535 GTAC GT^AC
SexAI 350 ACCTGGT A^CC[AT]GGT
SfaNI 126 GATGC GCATC(5/9)
SphI 604 GCATGC GCATG^C
StyI 61 CCATGG C^C[AT][AT]GG
Tsp45I 452 GTGAC ^GT[GC]AC
Tsp509I 363 AATT ^AATT
Van91I 195 CCAAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 338 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AlwI 339 GATCC GGATC(4/5)
338 GGATC
DpnII 339 GATC ^GATC
417 GATC
DraII 92 AGGGCCT [GA]G^G[ACGT]CC[CT]
305 AGGACCC
EcoO109I 92 AGGGCCT [GA]G^G[ACGT]CC[CT]
305 AGGACCC
MboI 339 GATC ^GATC
417 GATC
MslI 12 CACAGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
445 CACAATAGTG
NdeII 339 GATC ^GATC
417 GATC
NspI 466 GCATGT [GA]CATG^[CT]
604 GCATGC
Sau3AI 339 GATC ^GATC
417 GATC
SmlI 333 CTCAAG C^T[CT][GA]AG
630 CTTAAG
TspRI 139 CAGTG CA[GC]TG[ACGT][ACGT]^
160 CAGTG
XcmI 61 CCATGGCACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
542 CCATTTACAAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AvaII BmyI BsaJI BseRI BsiYI
BslI Bsp1286I BsrI BssKI BstNI
Cfr13I DdeI EcoRII HaeIII HphI
MaeIII MboII MnlI MseI MvaI
MwoI NlaIII NlaIV Sau96I ScrFI
SfcI Tru9I