Restriction map for
pFN21AE6468
##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AvaI AviII AvrII BanII BbrPI
BciVI BclI BglI BlnI BlpI
Bpu1102I BsaBI BsaWI BseAI BsoBI
BspDI BspEI BspHI BsrDI BssHII
Bst1107I BstBI BstZ17I Bsu36I CelII
ClaI CpoI DraI Eam1105I Eco47III
Eco81I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KspI
MamI MfeI MluI MroI MunI
NcoI NdeI NheI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspI SwaI TaqI
Tth111I TthHB8I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 332 GGTACC G^GTACC
AflII 1477 CTTAAG C^TTAAG
Asp718I 332 GGTACC G^GTACC
BalI 704 TGGCCA TGG^CCA
BamHI 354 GGATCC G^GATCC
BbeI 223 GGCGCC GGCGC^C
BbsI 1564 GTCTTC GAAGAC(2/6)
BbvCI 1455 GCTGAGG CCTCAGC(-5/-2)
BcgI 770 GCAGCCTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1653 CCCGG CC^[GC]GG
BfaI 1090 CTAG C^TAG
BfrI 1477 CTTAAG C^TTAAG
BglII 740 AGATCT A^GATCT
BpuAI 1564 GTCTTC GAAGAC(2/6)
BsaAI 1444 TACGTG [CT]AC^GT[GA]
BsaI 279 GGTCTC GGTCTC(1/5)
BsgI 496 GTGCAG GTGCAG(16/14)
BsiWI 59 CGTACG C^GTACG
Bsp1407I 674 TGTACA T^GTACA
BspLU11I 677 ACATGT A^CATGT
BsrBI 169 CCGCTC CCGCTC(-3/-3)
BsrGI 674 TGTACA T^GTACA
BssSI 1636 CTCGTG CACGAG(-5/-1)
BstAPI 198 GCAGCGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 388 GGTAACC G^GT[ACGT]ACC
BstPI 388 GGTAACC G^GT[ACGT]ACC
DraIII 1698 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1093 GACACGGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 337 CTCTTC CTCTTC(1/4)
EcoO65I 388 GGTAACC G^GT[ACGT]ACC
FauI 1056 CCCGC CCCGC(4/6)
HincII 1318 GTCAAC GT[CT]^[GA]AC
HindII 1318 GTCAAC GT[CT]^[GA]AC
KasI 223 GGCGCC G^GCGCC
KpnI 332 GGTACC GGTAC^C
Ksp632I 337 CTCTTC CTCTTC(1/4)
MaeI 1090 CTAG C^TAG
MluNI 704 TGGCCA TGG^CCA
MscI 704 TGGCCA TGG^CCA
NaeI 507 GCCGGC GCC^GGC
NarI 223 GGCGCC GG^CGCC
NciI 1653 CCCGG CC^[GC]GG
NgoMIV 507 GCCGGC G^CCGGC
NotI 12 GCGGCCGC GC^GGCCGC
PpuMI 1594 GGGACCC [GA]G^G[AT]CC[CT]
PstI 1071 CTGCAG CTGCA^G
ScaI 437 AGTACT AGT^ACT
SfoI 223 GGCGCC GGC^GCC
SspBI 674 TGTACA T^GTACA
StuI 716 AGGCCT AGG^CCT
Tsp509I 913 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 11 CGCG CG^CG
1389 CGCG
AspHI 616 GTGCTC G[AT]GC[AT]^C
1177 GTGCTC
BsiHKAI 616 GTGCTC G[AT]GC[AT]^C
1177 GTGCTC
BsmBI 1426 GAGACG CGTCTC(1/5)
1757 GAGACG
BsmI 164 GAATGC GAATGC(1/-1)
1120 GCATTC
BspMI 1280 ACCTGC ACCTGC(4/8)
1002 GCAGGT
BsrFI 507 GCCGGC [GA]^CCGG[CT]
1489 ACCGGC
BstUI 11 CGCG CG^CG
1389 CGCG
BstYI 354 GGATCC [GA]^GATC[CT]
740 AGATCT
Cfr10I 507 GCCGGC [GA]^CCGG[CT]
1489 ACCGGC
DraII 638 GGGGCCT [GA]G^G[ACGT]CC[CT]
1594 GGGACCC
EcoNI 996 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1281 CCTGCCCAAGG
EcoO109I 638 GGGGCCT [GA]G^G[ACGT]CC[CT]
1594 GGGACCC
EcoT14I 713 CCAAGG C^C[AT][AT]GG
1286 CCAAGG
HaeII 223 GGCGCC [GA]GCGC^[CT]
510 GGCGCT
MflI 354 GGATCC [GA]^GATC[CT]
740 AGATCT
MseI 1145 TTAA T^TAA
1478 TTAA
MvnI 11 CGCG CG^CG
1389 CGCG
PflMI 379 CCACTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
713 CCAAGGCCTGG
PleI 568 GAGTC GAGTC(4/5)
939 GAGTC
PvuII 700 CAGCTG CAG^CTG
1165 CAGCTG
SfcI 34 CTACAG C^T[GA][CT]AG
1071 CTGCAG
SmlI 1477 CTTAAG C^T[CT][GA]AG
1747 CTCAAG
SphI 203 GCATGC GCATG^C
1217 GCATGC
StyI 713 CCAAGG C^C[AT][AT]GG
1286 CCAAGG
TfiI 89 GATTC G^A[AT]TC
1081 GAATC
Tru9I 1145 TTAA T^TAA
1478 TTAA
Van91I 379 CCACTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
713 CCAAGGCCTGG
XhoII 354 GGATCC [GA]^GATC[CT]
740 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcyI AfaI AflIII AluI
AlwI AvaII BanI BbvI BmyI
BpmI BsaHI BsaJI BseRI BsiEI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EaeI EagI EclXI Eco52I
EcoRII Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI RsaI Sau3AI Sau96I
ScrFI SfaNI TseI Tsp45I TspRI
XcmI