Restriction map for
pFN21AE1921
##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII AccI AclI AcsI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
AspEI AspI AviII AvrII BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaBI
BsaI BsiEI BsiWI BsmI BspDI
BspHI BspLU11I BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KspI MamI MfeI MluI
MunI NciI NdeI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PpuMI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI StuI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 792 GGTACC G^GTACC
AccIII 1080 TCCGGA T^CCGGA
AocI 558 CCTGAGG CC^T[ACGT]AGG
Asp700I 756 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 792 GGTACC G^GTACC
AvaI 230 CTCGGG C^[CT]CG[GA]G
BalI 217 TGGCCA TGG^CCA
BamHI 830 GGATCC G^GATCC
BbrPI 1284 CACGTG CAC^GTG
BbvCI 1194 GCTGAGG CCTCAGC(-5/-2)
BfaI 1015 CTAG C^TAG
BsaAI 1284 CACGTG [CT]AC^GT[GA]
BsaWI 1080 TCCGGA [AT]^CCGG[AT]
BseAI 1080 TCCGGA T^CCGGA
BsmBI 735 CGTCTC CGTCTC(1/5)
BsoBI 230 CTCGGG C^[CT]CG[GA]G
Bsp1407I 719 TGTACA T^GTACA
BspEI 1080 TCCGGA T^CCGGA
BsrGI 719 TGTACA T^GTACA
BsrI 618 CCAGT ACTGG(1/-1)
BstXI 464 CCACCCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 830 GGATCC [GA]^GATC[CT]
Bsu36I 558 CCTGAGG CC^T[ACGT]AGG
Eco81I 558 CCTGAGG CC^T[ACGT]AGG
EcoNI 150 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 979 GATATC GAT^ATC
KpnI 792 GGTACC GGTAC^C
MaeI 1015 CTAG C^TAG
MaeII 1285 ACGT A^CGT
MflI 830 GGATCC [GA]^GATC[CT]
MluNI 217 TGGCCA TGG^CCA
MroI 1080 TCCGGA T^CCGGA
MscI 217 TGGCCA TGG^CCA
MseI 1091 TTAA T^TAA
NaeI 72 GCCGGC GCC^GGC
NgoMIV 72 GCCGGC G^CCGGC
NheI 1014 GCTAGC G^CTAGC
NspI 533 ACATGC [GA]CATG^[CT]
PflMI 809 CCATGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 610 GAGTC GAGTC(4/5)
PmaCI 1284 CACGTG CAC^GTG
PmlI 1284 CACGTG CAC^GTG
SacI 1117 GAGCTC GAGCT^C
SapI 273 GCTCTTC GCTCTTC(1/4)
SbfI 1221 CCTGCAGG CCTGCA^GG
ScaI 758 AGTACT AGT^ACT
SfaNI 941 GCATC GCATC(5/9)
SmlI 1008 CTCAAG C^T[CT][GA]AG
Sse8387I 1221 CCTGCAGG CCTGCA^GG
SspBI 719 TGTACA T^GTACA
SspI 668 AATATT AAT^ATT
TfiI 950 GAATC G^A[AT]TC
Tru9I 1091 TTAA T^TAA
Tsp509I 1087 AATT ^AATT
Van91I 809 CCATGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 830 GGATCC [GA]^GATC[CT]
XmnI 756 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 518 CGCG CG^CG
1315 CGCG
AlwNI 145 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
961 CAGAAACTG
ApaI 62 GGGCCC GGGCC^C
894 GGGCCC
AspHI 621 GTGCTC G[AT]GC[AT]^C
1117 GAGCTC
BbeI 49 GGCGCC GGCGC^C
1279 GGCGCC
BbsI 426 GAAGAC GAAGAC(2/6)
574 GTCTTC
BpuAI 426 GAAGAC GAAGAC(2/6)
574 GTCTTC
BsiHKAI 621 GTGCTC G[AT]GC[AT]^C
1117 GAGCTC
BsrBI 1335 CCGCTC CCGCTC(-3/-3)
751 GAGCGG
BsrDI 357 CATTGC GCAATG(2/0)
296 GCAATG
BstUI 518 CGCG CG^CG
1315 CGCG
DsaI 653 CCATGG C^C[GA][CT]GG
809 CCATGG
HinfI 610 GAGTC G^A[ACGT]TC
950 GAATC
KasI 49 GGCGCC G^GCGCC
1279 GGCGCC
MaeIII 110 GTGAC ^GT[ACGT]AC
992 GTGAC
MvnI 518 CGCG CG^CG
1315 CGCG
NarI 49 GGCGCC GG^CGCC
1279 GGCGCC
NcoI 653 CCATGG C^CATGG
809 CCATGG
PshAI 994 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1311 GACCCGCGTC
PvuII 163 CAGCTG CAG^CTG
309 CAGCTG
SfoI 49 GGCGCC GGC^GCC
1279 GGCGCC
TaqI 128 TCGA T^CGA
599 TCGA
Tsp45I 110 GTGAC ^GT[GC]AC
992 GTGAC
TthHB8I 128 TCGA T^CGA
599 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AvaII BanI BanII BbvI BmyI
BpmI BsaHI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrFI BssKI BstF5I BstNI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII HphI ItaI
Ksp632I MboI MboII MnlI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV PstI RsaI Sau3AI
Sau96I ScrFI SfcI StyI TseI
TspRI