##################################
# NO CUT ENZYMES (  213 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflIII      AgeI        
AhdI        Alw44I      AlwI        AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BbvI        BciVI       BclI        BfaI        
BglI        BglII       BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaJI       BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmAI       BsmBI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstNI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
ClaI        CpoI        DpnII       DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoRI       EcoRII      
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
Fnu4HI      FseI        FspI        HaeII       HaeIII      
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HpaI        ItaI        KasI        KpnI        
Ksp632I     KspI        MaeI        MaeII       MamI        
MboI        MboII       MfeI        MflI        MluI        
MluNI       MroI        MscI        MslI        MspA1I      
MunI        MvaI        MvnI        MwoI        NaeI        
NarI        NcoI        NdeI        NdeII       NgoMIV      
NheI        NlaIV       NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau3AI      
Sau96I      SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TseI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AcsI          196  AAATTT               [GA]^AATT[CT]
AfaI          212  GTAC                 GT^AC
AflII         322  CTTAAG               C^TTAAG
AlwNI          74  CAGGGACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          196  AAATTT               [GA]^AATT[CT]
Asp700I       180  GAAAGCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BcgI           30  CGACCCCTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI           69  CCCGG                CC^[GC]GG
BfrI          322  CTTAAG               C^TTAAG
BsiYI         229  CCTGAATACGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          229  CCTGAATACGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI          76  GGGAC                GGGAC(10/14)
BsrDI         147  CATTGC               GCAATG(2/0)
BsrI          282  CCAGT                ACTGG(1/-1)
BssKI          69  CCCGG                ^CC[ACGT]GG
BstEII        259  GGTGACC              G^GT[ACGT]ACC
BstF5I        226  CATCC                GGATG(2/0)
BstPI         259  GGTGACC              G^GT[ACGT]ACC
EcoO65I       259  GGTGACC              G^GT[ACGT]ACC
FokI          226  CATCC                GGATG(9/13)
HapII          70  CCGG                 C^CGG
HindIII       182  AAGCTT               A^AGCTT
HpaII          70  CCGG                 C^CGG
MseI          323  TTAA                 T^TAA
MspI           70  CCGG                 C^CGG
NciI           69  CCCGG                CC^[GC]GG
NlaIII        331  CATG                 CATG^
RsaI          212  GTAC                 GT^AC
ScrFI          69  CCCGG                CC^[ACGT]GG
SfaNI          43  GATGC                GCATC(5/9)
SmlI          322  CTTAAG               C^T[CT][GA]AG
TaqI           29  TCGA                 T^CGA
Tru9I         323  TTAA                 T^TAA
Tsp509I       197  AATT                 ^AATT
TspRI         128  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I        29  TCGA                 T^CGA
XmnI          180  GAAAGCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (    6 enzymes) #
###################################

DdeI           50  CTAAG                C^T[ACGT]AG
               80  CTGAG               
HinfI         101  GAATC                G^A[ACGT]TC
              335  GATTC               
HphI           55  GGTGA                GGTGA(8/7)
              259  GGTGA               
MaeIII         56  GTGAC                ^GT[ACGT]AC
              260  GTGAC               
TfiI          101  GAATC                G^A[AT]TC
              335  GATTC               
Tsp45I         56  GTGAC                ^GT[GC]AC
              260  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (    2 enzymes) #
#########################################

AluI        MnlI