Restriction map for
pFN21AE6608
##################################
# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BamHI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaHI
BsaI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Cac8I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FseI
FspI HaeII Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MslI
MunI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XcmI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccII 446 CGCG CG^CG
AcsI 657 AAATTT [GA]^AATT[CT]
AfaI 217 GTAC GT^AC
AflIII 198 ACGTGT A^C[GA][CT]GT
AlwI 144 GGATC GGATC(4/5)
AocI 492 CCTGAGG CC^T[ACGT]AGG
ApoI 657 AAATTT [GA]^AATT[CT]
AvaI 830 CCCGAG C^[CT]CG[GA]G
AvaII 113 GGACC G^G[AT]CC
BanI 518 GGCACC G^G[CT][GA]CC
BclI 80 TGATCA T^GATCA
BcnI 522 CCGGG CC^[GC]GG
BfaI 194 CTAG C^TAG
BsaAI 803 TACGTG [CT]AC^GT[GA]
BsaWI 75 TCCGGT [AT]^CCGG[AT]
BseRI 495 GAGGAG GAGGAG(10/8)
BsmAI 47 GAGAC GTCTC(1/5)
BsoBI 830 CCCGAG C^[CT]CG[GA]G
BspHI 621 TCATGA T^CATGA
BsrDI 859 GCAATG GCAATG(2/0)
BsrI 696 ACTGG ACTGG(1/-1)
BstUI 446 CGCG CG^CG
BstYI 144 GGATCT [GA]^GATC[CT]
Bsu36I 492 CCTGAGG CC^T[ACGT]AGG
Eco81I 492 CCTGAGG CC^T[ACGT]AGG
EcoT14I 816 CCAAGG C^C[AT][AT]GG
FbaI 80 TGATCA T^GATCA
HgaI 444 GACGC GACGC(5/10)
MaeI 194 CTAG C^TAG
MflI 144 GGATCT [GA]^GATC[CT]
MseI 152 TTAA T^TAA
MspA1I 663 CAGCTG C[AC]G^C[GT]G
MvnI 446 CGCG CG^CG
MwoI 856 GCAGCAATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 522 CCGGG CC^[GC]GG
NdeI 730 CATATG CA^TATG
PflMI 135 CCAACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 112 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 663 CAGCTG CAG^CTG
RcaI 621 TCATGA T^CATGA
RsaI 217 GTAC GT^AC
SexAI 626 ACCAGGT A^CC[AT]GGT
StyI 816 CCAAGG C^C[AT][AT]GG
TaqI 558 TCGA T^CGA
Tru9I 152 TTAA T^TAA
Tsp509I 658 AATT ^AATT
TthHB8I 558 TCGA T^CGA
Van91I 135 CCAACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 193 TCTAGA T^CTAGA
XhoII 144 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AluI 664 AGCT AG^CT
737 AGCT
BalI 765 TGGCCA TGG^CCA
890 TGGCCA
BanII 393 GGGCTC G[GA]GC[CT]^C
833 GAGCCC
BpmI 172 CTCCAG CTGGAG(16/14)
370 CTGGAG
BspMI 115 ACCTGC ACCTGC(4/8)
708 ACCTGC
CfoI 447 GCGC GCG^C
565 GCGC
DraII 112 GGGACCT [GA]G^G[ACGT]CC[CT]
524 GGGGCCT
EaeI 765 TGGCCA [CT]^GGCC[GA]
890 TGGCCA
EarI 529 CTCTTC CTCTTC(1/4)
880 GAAGAG
EcoO109I 112 GGGACCT [GA]G^G[ACGT]CC[CT]
524 GGGGCCT
HapII 76 CCGG C^CGG
522 CCGG
HhaI 447 GCGC GCG^C
565 GCGC
HinP1I 447 GCGC G^CGC
565 GCGC
HpaII 76 CCGG C^CGG
522 CCGG
HphI 78 GGTGA GGTGA(8/7)
424 TCACC
Ksp632I 529 CTCTTC CTCTTC(1/4)
880 GAAGAG
MluNI 765 TGGCCA TGG^CCA
890 TGGCCA
MscI 765 TGGCCA TGG^CCA
890 TGGCCA
MspI 76 CCGG C^CGG
522 CCGG
PleI 243 GAGTC GAGTC(4/5)
373 GAGTC
Tsp45I 423 GTCAC ^GT[GC]AC
775 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI BbvI BmyI BsaJI BsiYI
BslI BsmFI Bsp1286I BssKI BstF5I
BstNI Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HinfI ItaI
MaeII MaeIII MboI MboII MnlI
MvaI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI TfiI TseI
TspRI