Restriction map for
pFN21AE3038
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# NO CUT ENZYMES ( 169 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanII BbeI BbrPI BbvCI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiHKAI BsiWI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BssSI
Bst1107I BstAPI BstBI BstF5I BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT14I
FbaI FokI FseI FspI HaeII
Hin1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MaeII MamI MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI Tsp509I Tth111I TthHB8I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccII 16 CGCG CG^CG
AlwI 503 GATCC GGATC(4/5)
BanI 303 GGTGCC G^G[CT][GA]CC
BbsI 253 GTCTTC GAAGAC(2/6)
BmyI 304 GTGCCC G[AGT]GC[ACT]^C
BpuAI 253 GTCTTC GAAGAC(2/6)
BseRI 4 GAGGAG GAGGAG(10/8)
BsgI 118 CTGCAC GTGCAG(16/14)
BsiEI 96 CGACCG CG[GA][CT]^CG
BsmAI 388 GTCTC GTCTC(1/5)
BsmBI 387 CGTCTC CGTCTC(1/5)
Bsp1286I 304 GTGCCC G[AGT]GC[ACT]^C
BsrBI 21 CCGCTC CCGCTC(-3/-3)
BsrI 239 ACTGG ACTGG(1/-1)
BssHII 15 GCGCGC G^CGCGC
BstEII 453 GGTCACC G^GT[ACGT]ACC
BstPI 453 GGTCACC G^GT[ACGT]ACC
BstUI 16 CGCG CG^CG
BstYI 502 AGATCC [GA]^GATC[CT]
EaeI 137 TGGCCG [CT]^GGCC[GA]
EcoNI 284 CCTACACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 453 GGTCACC G^GT[ACGT]ACC
EcoT22I 377 ATGCAT ATGCA^T
FauI 20 CCCGC CCCGC(4/6)
HgaI 168 GACGC GACGC(5/10)
HinfI 294 GATTC G^A[ACGT]TC
MflI 502 AGATCC [GA]^GATC[CT]
MseI 332 TTAA T^TAA
MslI 273 CATAACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 16 CGCG CG^CG
NsiI 377 ATGCAT ATGCA^T
PflMI 202 CCAGCATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 420 CAGCTG CAG^CTG
ScaI 188 AGTACT AGT^ACT
TfiI 294 GATTC G^A[AT]TC
Tru9I 332 TTAA T^TAA
Van91I 202 CCAGCATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 202 CCAGCATTTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 502 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 189 GTAC GT^AC
354 GTAC
BcgI 80 GCAGCAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
338 GCAGTGCCTTCG
BcnI 99 CCGGG CC^[GC]GG
195 CCGGG
BsaJI 299 CCCAGG C^C[ACGT][ACGT]GG
307 CCCTGG
BsmFI 401 GGGAC GGGAC(10/14)
199 GTCCC
CfoI 15 GCGC GCG^C
17 GCGC
DdeI 185 CTGAG C^T[ACGT]AG
395 CTGAG
DpnII 144 GATC ^GATC
503 GATC
DraII 197 GGGTCCC [GA]G^G[ACGT]CC[CT]
242 GGGTCCT
EcoO109I 197 GGGTCCC [GA]G^G[ACGT]CC[CT]
242 GGGTCCT
HapII 99 CCGG C^CGG
195 CCGG
HhaI 15 GCGC GCG^C
17 GCGC
HinP1I 15 GCGC G^CGC
17 GCGC
HpaII 99 CCGG C^CGG
195 CCGG
MboI 144 GATC ^GATC
503 GATC
MspA1I 365 CCGCTG C[AC]G^C[GT]G
420 CAGCTG
MspI 99 CCGG C^CGG
195 CCGG
MwoI 77 GCAGCAGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
329 GCTTTAACGGC
NciI 99 CCGGG CC^[GC]GG
195 CCGGG
NdeII 144 GATC ^GATC
503 GATC
NlaIII 219 CATG CATG^
497 CATG
PpuMI 197 GGGTCCC [GA]G^G[AT]CC[CT]
242 GGGTCCT
RsaI 189 GTAC GT^AC
354 GTAC
Sau3AI 144 GATC ^GATC
503 GATC
SfaNI 376 GATGC GCATC(5/9)
379 GCATC
TspRI 317 CAGTG CA[GC]TG[ACGT][ACGT]^
339 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI AluI AvaII BbvI BsiYI
BslI BssKI BstNI Cac8I Cfr13I
EcoRII Fnu4HI HaeIII HphI ItaI
MaeIII MboII MnlI MvaI NlaIV
Sau96I ScrFI TseI Tsp45I