Restriction map for
pFN21AB5810
##################################
# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp700I
AspEI AspI AviII AvrII BamHI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BspEI BspHI BspMI
BsrBI BsrFI BssHII BstAPI BstEII
BstPI BstUI Bsu36I CelII Cfr10I
CpoI DraI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HapII HgaI
Hin1I HindIII HpaI HpaII KasI
Ksp632I KspI MamI MluI MroI
MslI MspI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SapI SbfI
ScaI SfcI SfiI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
StyI SwaI Tth111I Van91I XbaI
XcmI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 269 GGTACC G^GTACC
AflIII 1094 ACATGT A^C[GA][CT]GT
AseI 373 ATTAAT AT^TAAT
AsnI 373 ATTAAT AT^TAAT
Asp718I 269 GGTACC G^GTACC
AvaI 224 CTCGAG C^[CT]CG[GA]G
BalI 671 TGGCCA TGG^CCA
BanI 269 GGTACC G^G[CT][GA]CC
BbsI 69 GTCTTC GAAGAC(2/6)
BbvI 135 GCAGC GCAGC(8/12)
BfaI 1286 CTAG C^TAG
BpuAI 69 GTCTTC GAAGAC(2/6)
BsaI 989 GAGACC GGTCTC(1/5)
BsgI 1151 GTGCAG GTGCAG(16/14)
BsmI 496 GAATGC GAATGC(1/-1)
BsoBI 224 CTCGAG C^[CT]CG[GA]G
Bsp1407I 230 TGTACA T^GTACA
BspDI 392 ATCGAT AT^CGAT
BspLU11I 1094 ACATGT A^CATGT
BsrDI 1040 CATTGC GCAATG(2/0)
BsrGI 230 TGTACA T^GTACA
BssSI 1336 CTCGTG CACGAG(-5/-1)
Bst1107I 807 GTATAC GTA^TAC
BstBI 486 TTCGAA TT^CGAA
BstXI 1298 CCAGCTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 807 GTATAC GTA^TAC
CfoI 287 GCGC GCG^C
ClaI 392 ATCGAT AT^CGAT
DraII 998 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 675 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 622 GACCATCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 671 TGGCCA [CT]^GGCC[GA]
EcoNI 756 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 998 AGGACCT [GA]G^G[ACGT]CC[CT]
HaeII 286 AGCGCC [GA]GCGC^[CT]
HhaI 287 GCGC GCG^C
HinP1I 287 GCGC G^CGC
HincII 407 GTCGAC GT[CT]^[GA]AC
HindII 407 GTCGAC GT[CT]^[GA]AC
KpnI 269 GGTACC GGTAC^C
MaeI 1286 CTAG C^TAG
MaeII 711 ACGT A^CGT
MfeI 158 CAATTG C^AATTG
MluNI 671 TGGCCA TGG^CCA
MscI 671 TGGCCA TGG^CCA
MunI 158 CAATTG C^AATTG
NspV 486 TTCGAA TT^CGAA
PaeR7I 224 CTCGAG C^TCGAG
PpuMI 998 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 373 ATTAAT AT^TAAT
SalI 407 GTCGAC G^TCGAC
SexAI 802 ACCAGGT A^CC[AT]GGT
SfuI 486 TTCGAA TT^CGAA
SspBI 230 TGTACA T^GTACA
StuI 957 AGGCCT AGG^CCT
TseI 135 GCAGC G^C[AT]GC
XhoI 224 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 407 GTCGAC GT^[AC][GT]AC
807 GTATAC
AfaI 231 GTAC GT^AC
270 GTAC
AlwI 549 GATCC GGATC(4/5)
1189 GGATC
AlwNI 285 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
922 CAGCTCCTG
AspHI 171 GAGCAC G[AT]GC[AT]^C
1276 GAGCAC
BglII 1106 AGATCT A^GATCT
1125 AGATCT
BmyI 171 GAGCAC G[AGT]GC[ACT]^C
1276 GAGCAC
BsaJI 34 CCTGGG C^C[ACGT][ACGT]GG
642 CCTGGG
BsiHKAI 171 GAGCAC G[AT]GC[AT]^C
1276 GAGCAC
BsmAI 481 GAGAC GTCTC(1/5)
989 GAGAC
BsmFI 653 GGGAC GGGAC(10/14)
193 GTCCC
Bsp1286I 171 GAGCAC G[AGT]GC[ACT]^C
1276 GAGCAC
EcoRI 609 GAATTC G^AATTC
1224 GAATTC
Fnu4HI 135 GCAGC GC^[ACGT]GC
766 GCCGC
ItaI 135 GCAGC GC^[ACGT]GC
766 GCCGC
MspA1I 521 CAGCTG C[AC]G^C[GT]G
589 CAGCTG
NlaIV 269 GGTACC GG[ACGT]^[ACGT]CC
1008 GGAACC
PvuII 521 CAGCTG CAG^CTG
589 CAGCTG
RsaI 231 GTAC GT^AC
270 GTAC
SmlI 224 CTCGAG C^T[CT][GA]AG
1003 CTTGAG
SphI 77 GCATGC GCATG^C
103 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AcsI AluI ApoI AvaII
BpmI BsiYI BslI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII EcoRII FokI HaeIII
HinfI HphI MaeIII MboI MboII
MflI MnlI MseI MvaI MwoI
NdeII NlaIII NspI PleI Sau3AI
Sau96I ScrFI SfaNI TaqI TfiI
Tru9I Tsp45I Tsp509I TspRI TthHB8I
XhoII