Restriction map for
pFN21AE2396
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AgeI
AhdI AlwI AlwNI AocI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiWI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DpnII DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I FauI
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MaeI MamI MboI
MfeI MflI MluI MroI MseI
MslI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NdeII
NgoMIV NheI NotI NruI PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
Sau3AI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AciI 415 CCGC CCGC(-3/-1)
AflIII 277 ACATGT A^C[GA][CT]GT
Alw44I 624 GTGCAC G^TGCAC
ApaLI 624 GTGCAC G^TGCAC
AspHI 624 GTGCAC G[AT]GC[AT]^C
BalI 2 TGGCCA TGG^CCA
BanII 649 GAGCCC G[GA]GC[CT]^C
BbvI 121 GCTGC GCAGC(8/12)
BciVI 540 GTATCC GTATCC(6/5)
BpmI 387 CTCCAG CTGGAG(16/14)
BsaJI 5 CCAAGG C^C[ACGT][ACGT]GG
BsgI 122 CTGCAC GTGCAG(16/14)
BsiHKAI 624 GTGCAC G[AT]GC[AT]^C
BsmBI 741 GAGACG CGTCTC(1/5)
BsmFI 680 GGGAC GGGAC(10/14)
BspLU11I 277 ACATGT A^CATGT
BstBI 663 TTCGAA TT^CGAA
BstF5I 243 CATCC GGATG(2/0)
DraII 680 GGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 2 TGGCCA [CT]^GGCC[GA]
EarI 674 GAAGAG CTCTTC(1/4)
EcoO109I 680 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 708 GAATTC G^AATTC
EcoT14I 5 CCAAGG C^C[AT][AT]GG
EcoT22I 290 ATGCAT ATGCA^T
Fnu4HI 121 GCTGC GC^[ACGT]GC
FokI 243 CATCC GGATG(9/13)
HaeIII 3 GGCC GG^CC
HinfI 457 GAATC G^A[ACGT]TC
ItaI 121 GCTGC GC^[ACGT]GC
Ksp632I 674 GAAGAG CTCTTC(1/4)
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MspA1I 250 CAGCTG C[AC]G^C[GT]G
NsiI 290 ATGCAT ATGCA^T
NspI 277 ACATGT [GA]CATG^[CT]
NspV 663 TTCGAA TT^CGAA
PpuMI 680 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 250 CAGCTG CAG^CTG
SfaNI 14 GATGC GCATC(5/9)
SfcI 247 CTACAG C^T[GA][CT]AG
SfuI 663 TTCGAA TT^CGAA
StyI 5 CCAAGG C^C[AT][AT]GG
TaqI 664 TCGA T^CGA
TfiI 457 GAATC G^A[AT]TC
TseI 121 GCTGC G^C[AT]GC
Tsp45I 498 GTCAC ^GT[GC]AC
TthHB8I 664 TCGA T^CGA
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# TWO CUT ENZYMES ( 25 enzymes) #
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Asp700I 457 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
560 GAAGACTTTC
AvaII 366 GGACC G^G[AT]CC
681 GGACC
BbsI 46 GAAGAC GAAGAC(2/6)
560 GAAGAC
BmyI 624 GTGCAC G[AGT]GC[ACT]^C
649 GAGCCC
BpuAI 46 GAAGAC GAAGAC(2/6)
560 GAAGAC
BsiYI 445 CCAGGACAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
597 CCCAGCAGTGG
BslI 445 CCAGGACAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
597 CCCAGCAGTGG
BsmAI 741 GAGAC GTCTC(1/5)
532 GTCTC
Bsp1286I 624 GTGCAC G[AGT]GC[ACT]^C
649 GAGCCC
BspMI 330 ACCTGC ACCTGC(4/8)
107 GCAGGT
BssKI 342 CCAGG ^CC[ACGT]GG
445 CCAGG
BstNI 342 CCAGG CC^[AT]GG
445 CCAGG
Cac8I 592 GCAAGC GC[ACGT]^[ACGT]GC
631 GCCAGC
Cfr13I 366 GGACC G^G[ACGT]CC
681 GGACC
EcoRII 342 CCAGG ^CC[AT]GG
445 CCAGG
EcoRV 238 GATATC GAT^ATC
520 GATATC
MaeII 618 ACGT A^CGT
744 ACGT
MaeIII 266 GTTAC ^GT[ACGT]AC
498 GTCAC
MvaI 342 CCAGG CC^[AT]GG
445 CCAGG
MwoI 592 GCAAGCCCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
626 GCACAGCCAGC
NlaIII 128 CATG CATG^
278 CATG
Sau96I 366 GGACC G^G[ACGT]CC
681 GGACC
ScrFI 342 CCAGG CC^[ACGT]GG
445 CCAGG
TspRI 155 CACTG CA[GC]TG[ACGT][ACGT]^
602 CAGTG
XmnI 457 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
560 GAAGACTTTC
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# 3 OR MORE CUT ENZYMES ( 9 enzymes) #
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AcsI AluI ApoI DdeI HphI
MboII MnlI NlaIV Tsp509I