Restriction map for
pFN21AE3567
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# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AflIII AgeI Aor51HI
AscI AseI AsnI Asp718I AviII
AvrII BbrPI BcgI BciVI BlnI
BsaAI BsaBI BsiEI BsiWI BsmI
Bsp1407I BspDI BspLU11I BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraIII DrdI EagI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoT22I FseI FspI HpaI KpnI
KspI MamI MfeI MluI MspA1I
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI PvuI PvuII RsrII
SacII SalI SapI SbfI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 127 GTCTAC GT^[AC][GT]AC
AccII 2210 CGCG CG^CG
AccIII 597 TCCGGA T^CCGGA
AflII 2250 CTTAAG C^TTAAG
AhdI 745 GACGACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2786 GTGCAC G^TGCAC
AlwNI 2677 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1677 CCTCAGG CC^T[ACGT]AGG
ApaI 1239 GGGCCC GGGCC^C
ApaLI 2786 GTGCAC G^TGCAC
AspEI 745 GACGACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 852 TGGCCA TGG^CCA
BamHI 2883 GGATCC G^GATCC
BbeI 24 GGCGCC GGCGC^C
BbvCI 2701 CCTCAGC CCTCAGC(-5/-2)
BclI 1871 TGATCA T^GATCA
BfrI 2250 CTTAAG C^TTAAG
BglI 100 GCCCAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1284 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1284 GCTGAGC GC^T[ACGT]AGC
BsaWI 597 TCCGGA [AT]^CCGG[AT]
BseAI 597 TCCGGA T^CCGGA
BspEI 597 TCCGGA T^CCGGA
BspMI 530 ACCTGC ACCTGC(4/8)
BsrBI 1268 CCGCTC CCGCTC(-3/-3)
BsrFI 3 GCCGGT [GA]^CCGG[CT]
BssSI 976 CTCGTG CACGAG(-5/-1)
BstUI 2210 CGCG CG^CG
Bsu36I 1677 CCTCAGG CC^T[ACGT]AGG
CelII 1284 GCTGAGC GC^T[ACGT]AGC
Cfr10I 3 GCCGGT [GA]^CCGG[CT]
DsaI 1784 CCATGG C^C[GA][CT]GG
EaeI 852 TGGCCA [CT]^GGCC[GA]
Eam1105I 745 GACGACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1677 CCTCAGG CC^T[ACGT]AGG
EcoRI 274 GAATTC G^AATTC
EcoRV 2043 GATATC GAT^ATC
FauI 2819 GCGGG CCCGC(4/6)
FbaI 1871 TGATCA T^GATCA
HaeII 24 GGCGCC [GA]GCGC^[CT]
HindIII 2253 AAGCTT A^AGCTT
KasI 24 GGCGCC G^GCGCC
MluNI 852 TGGCCA TGG^CCA
MroI 597 TCCGGA T^CCGGA
MscI 852 TGGCCA TGG^CCA
MvnI 2210 CGCG CG^CG
NarI 24 GGCGCC GG^CGCC
NcoI 1784 CCATGG C^CATGG
PpuMI 469 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 2422 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2921 GAGCTC GAGCT^C
SfoI 24 GGCGCC GGC^GCC
SspI 1526 AATATT AAT^ATT
XcmI 95 CCAATGCCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcyI 24 GGCGCC G[GA]^CG[CT]C
3060 GACGCC
AspI 442 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
494 GACGGAGTC
AvaI 1776 CTCGGG C^[CT]CG[GA]G
2271 CTCGGG
BglII 1343 AGATCT A^GATCT
1634 AGATCT
BsaHI 24 GGCGCC G[GA]^CG[CT]C
3060 GACGCC
BsgI 1356 CTGCAC GTGCAG(16/14)
2190 CTGCAC
BsmBI 1317 CGTCTC CGTCTC(1/5)
492 GAGACG
BsoBI 1776 CTCGGG C^[CT]CG[GA]G
2271 CTCGGG
BsrDI 783 CATTGC GCAATG(2/0)
740 GCAATG
BstXI 2910 CCAACGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3205 CCAGGATCTTGG
DraI 658 TTTAAA TTT^AAA
819 TTTAAA
DraII 469 GGGACCC [GA]G^G[ACGT]CC[CT]
1238 GGGGCCC
EcoO109I 469 GGGACCC [GA]G^G[ACGT]CC[CT]
1238 GGGGCCC
Hin1I 24 GGCGCC G[GA]^CG[CT]C
3060 GACGCC
HincII 1549 GTCAAC GT[CT]^[GA]AC
3057 GTTGAC
HindII 1549 GTCAAC GT[CT]^[GA]AC
3057 GTTGAC
PflFI 442 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
494 GACGGAGTC
ScaI 369 AGTACT AGT^ACT
1054 AGTACT
SmlI 1507 CTCAAG C^T[CT][GA]AG
2250 CTTAAG
Tth111I 442 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
494 GACGGAGTC
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# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AclI AcsI AfaI AluI
AlwI ApoI Asp700I AspHI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI Psp1406I PstI
RcaI RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmnI