Restriction map for
pFN21AE6602
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccI AccIII AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BbvCI BcgI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BspLU11I
BsrBI BsrDI BsrFI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HinfI HpaI KasI KpnI KspI
MaeI MamI MfeI MflI MluI
MluNI MroI MscI MseI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
PvuI RsrII SacI SacII SalI
SapI SbfI SexAI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspI SwaI TfiI Tru9I Tsp509I
Tth111I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccII 136 CGCG CG^CG
AclI 429 AACGTT AA^CGTT
AlwI 150 GGATC GGATC(4/5)
BciVI 309 GGATAC GTATCC(6/5)
BglI 115 GCCCCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 598 CACGTA [CT]AC^GT[GA]
BsgI 523 CTGCAC GTGCAG(16/14)
BsmAI 277 GAGAC GTCTC(1/5)
Bsp1407I 440 TGTACA T^GTACA
BspHI 548 TCATGA T^CATGA
BspMI 623 ACCTGC ACCTGC(4/8)
BsrGI 440 TGTACA T^GTACA
BstEII 78 GGTGACC G^GT[ACGT]ACC
BstF5I 680 CATCC GGATG(2/0)
BstPI 78 GGTGACC G^GT[ACGT]ACC
BstUI 136 CGCG CG^CG
CfoI 543 GCGC GCG^C
DdeI 22 CTTAG C^T[ACGT]AG
EcoO65I 78 GGTGACC G^GT[ACGT]ACC
FokI 680 CATCC GGATG(9/13)
HhaI 543 GCGC GCG^C
HinP1I 543 GCGC G^CGC
HindIII 557 AAGCTT A^AGCTT
HphI 78 GGTGA GGTGA(8/7)
MvnI 136 CGCG CG^CG
PflMI 294 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 191 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 429 AACGTT AA^CGTT
PvuII 88 CAGCTG CAG^CTG
RcaI 548 TCATGA T^CATGA
ScaI 287 AGTACT AGT^ACT
SfiI 114 GGCCCCCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 581 GCATGC GCATG^C
SspBI 440 TGTACA T^GTACA
StuI 674 AGGCCT AGG^CCT
Tsp45I 79 GTGAC ^GT[GC]AC
Van91I 294 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 597 CCACGTACTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AlwNI 85 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
88 CAGCTGCTG
ApaI 122 GGGCCC GGGCC^C
569 GGGCCC
BanI 374 GGCACC G^G[CT][GA]CC
610 GGCACC
BcnI 125 CCCGG CC^[GC]GG
210 CCGGG
EarI 574 CTCTTC CTCTTC(1/4)
283 GAAGAG
EcoNI 198 CCTCCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
327 CCTCCAGAAGG
EcoT14I 110 CCAAGG C^C[AT][AT]GG
665 CCAAGG
HapII 126 CCGG C^CGG
210 CCGG
HpaII 126 CCGG C^CGG
210 CCGG
Ksp632I 574 CTCTTC CTCTTC(1/4)
283 GAAGAG
MslI 216 CACTCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
526 CACTGCTGTG
MspA1I 88 CAGCTG C[AC]G^C[GT]G
564 CCGCTG
MspI 126 CCGG C^CGG
210 CCGG
NciI 125 CCCGG CC^[GC]GG
210 CCGGG
NspI 54 GCATGT [GA]CATG^[CT]
581 GCATGC
PstI 625 CTGCAG CTGCA^G
709 CTGCAG
SmlI 187 CTTGAG C^T[CT][GA]AG
202 CTCAAG
StyI 110 CCAAGG C^C[AT][AT]GG
665 CCAAGG
TaqI 153 TCGA T^CGA
638 TCGA
TthHB8I 153 TCGA T^CGA
638 TCGA
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AfaI AluI AvaII BanII
BbvI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmFI Bsp1286I BssKI
BstNI Cac8I Cfr13I DpnII DraII
EcoO109I EcoRII Fnu4HI HaeIII ItaI
MaeII MaeIII MboI MboII MnlI
MvaI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TseI TspRI