Restriction map for
pFN21AE0658
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI AocI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AvaI AvrII BamHI
BbvCI BcgI BciVI BclI BfrI
BglI BlnI BsaBI BsaI BsaWI
BseAI BsiWI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrDI BsrGI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI Eam1105I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HinfI HpaI
KpnI MaeIII MamI MfeI MluI
MroI MseI MunI NaeI NcoI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PleI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacI SalI SapI
SbfI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tru9I Tsp45I
Tsp509I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccI 622 GTCTAC GT^[AC][GT]AC
AfaI 348 GTAC GT^AC
Alw44I 514 GTGCAC G^TGCAC
Aor51HI 1025 AGCGCT AGC^GCT
ApaI 319 GGGCCC GGGCC^C
ApaLI 514 GTGCAC G^TGCAC
AspI 544 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 191 TGCGCA TGC^GCA
BanI 726 GGCGCC G^G[CT][GA]CC
BanII 319 GGGCCC G[GA]GC[CT]^C
BbeI 726 GGCGCC GGCGC^C
BbrPI 477 CACGTG CAC^GTG
BcnI 61 CCGGG CC^[GC]GG
BfaI 616 CTAG C^TAG
BglII 677 AGATCT A^GATCT
BlpI 159 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 159 GCTGAGC GC^T[ACGT]AGC
BsaAI 477 CACGTG [CT]AC^GT[GA]
BseRI 1140 GAGGAG GAGGAG(10/8)
BsmBI 1075 GAGACG CGTCTC(1/5)
BsmI 168 GAATGC GAATGC(1/-1)
BspHI 230 TCATGA T^CATGA
BsrBI 162 GAGCGG CCGCTC(-3/-3)
BsrFI 633 GCCGGT [GA]^CCGG[CT]
BsrI 23 ACTGG ACTGG(1/-1)
BssSI 1018 CTCGTG CACGAG(-5/-1)
BstAPI 684 GCAGAACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 159 GCTGAGC GC^T[ACGT]AGC
Cfr10I 633 GCCGGT [GA]^CCGG[CT]
EagI 719 CGGCCG C^GGCCG
EclXI 719 CGGCCG C^GGCCG
Eco47III 1025 AGCGCT AGC^GCT
Eco52I 719 CGGCCG C^GGCCG
FspI 191 TGCGCA TGC^GCA
HphI 302 TCACC GGTGA(8/7)
KasI 726 GGCGCC G^GCGCC
KspI 94 CCGCGG CCGC^GG
MaeI 616 CTAG C^TAG
MaeII 478 ACGT A^CGT
NarI 726 GGCGCC GG^CGCC
NciI 61 CCGGG CC^[GC]GG
NheI 615 GCTAGC G^CTAGC
PflFI 544 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 477 CACGTG CAC^GTG
PmlI 477 CACGTG CAC^GTG
PpuMI 585 GGGACCC [GA]G^G[AT]CC[CT]
PstI 707 CTGCAG CTGCA^G
PvuII 963 CAGCTG CAG^CTG
RcaI 230 TCATGA T^CATGA
RsaI 348 GTAC GT^AC
SacII 94 CCGCGG CCGC^GG
ScaI 347 AGTACT AGT^ACT
SexAI 254 ACCTGGT A^CC[AT]GGT
SfcI 707 CTGCAG C^T[GA][CT]AG
SfoI 726 GGCGCC GGC^GCC
SmlI 697 CTCAAG C^T[CT][GA]AG
StuI 593 AGGCCT AGG^CCT
TaqI 554 TCGA T^CGA
Tth111I 544 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 554 TCGA T^CGA
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# TWO CUT ENZYMES ( 16 enzymes) #
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BbsI 550 GTCTTC GAAGAC(2/6)
568 GTCTTC
BpmI 1131 CTCCAG CTGGAG(16/14)
24 CTGGAG
BpuAI 550 GTCTTC GAAGAC(2/6)
568 GTCTTC
BsgI 880 GTGCAG GTGCAG(16/14)
1021 GTGCAG
BsiEI 456 CGACCG CG[GA][CT]^CG
719 CGGCCG
BsmAI 1075 GAGAC GTCTC(1/5)
140 GTCTC
DraII 107 AGGCCCT [GA]G^G[ACGT]CC[CT]
585 GGGACCC
DsaI 94 CCGCGG C^C[GA][CT]GG
1160 CCACGG
EarI 220 CTCTTC CTCTTC(1/4)
1006 CTCTTC
EcoO109I 107 AGGCCCT [GA]G^G[ACGT]CC[CT]
585 GGGACCC
FauI 93 CCCGC CCCGC(4/6)
164 GCGGG
Ksp632I 220 CTCTTC CTCTTC(1/4)
1006 CTCTTC
MslI 834 CATCATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
936 CATCGTCATG
NspI 365 GCATGT [GA]CATG^[CT]
986 ACATGC
SfaNI 901 GATGC GCATC(5/9)
245 GCATC
TspRI 266 CAGTG CA[GC]TG[ACGT][ACGT]^
467 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI AspHI AvaII BalI BbvI
BmyI BsaHI BsaJI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BspMI BssHII
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII ItaI MboI
MboII MflI MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI StyI TseI XhoII