Restriction map for
pFN21AE3057
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AfaI AflII AflIII AhdI AlwNI
AocI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AvaI AviII
AvrII BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaI BseAI
BseRI BsgI BsiEI BsiWI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraII DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HinfI
HpaI KasI KpnI MamI MfeI
MflI MluI MroI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PaeR7I PflMI PleI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PstI
PvuI RsaI RsrII SacI SalI
SbfI ScaI SfcI SfiI SfoI
SfuI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AcsI 330 GAATTT [GA]^AATT[CT]
AcyI 589 GACGCC G[GA]^CG[CT]C
AgeI 316 ACCGGT A^CCGGT
Alw44I 202 GTGCAC G^TGCAC
AlwI 192 GGATC GGATC(4/5)
ApaLI 202 GTGCAC G^TGCAC
ApoI 330 GAATTT [GA]^AATT[CT]
AspI 382 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 438 TGGCCA TGG^CCA
BanI 319 GGTGCC G^G[CT][GA]CC
BanII 17 GGGCTC G[GA]GC[CT]^C
BbvI 418 GCTGC GCAGC(8/12)
BfaI 340 CTAG C^TAG
BsaHI 589 GACGCC G[GA]^CG[CT]C
BspHI 500 TCATGA T^CATGA
BspMI 206 ACCTGC ACCTGC(4/8)
BsrBI 472 GAGCGG CCGCTC(-3/-3)
BsrFI 316 ACCGGT [GA]^CCGG[CT]
Cfr10I 316 ACCGGT [GA]^CCGG[CT]
DraIII 196 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 95 CCGCGG C^C[GA][CT]GG
EaeI 438 TGGCCA [CT]^GGCC[GA]
EarI 54 GAAGAG CTCTTC(1/4)
EcoT14I 323 CCAAGG C^C[AT][AT]GG
HgaI 589 GACGC GACGC(5/10)
Hin1I 589 GACGCC G[GA]^CG[CT]C
HphI 314 TCACC GGTGA(8/7)
Ksp632I 54 GAAGAG CTCTTC(1/4)
KspI 95 CCGCGG CCGC^GG
MaeI 340 CTAG C^TAG
MluNI 438 TGGCCA TGG^CCA
MscI 438 TGGCCA TGG^CCA
MslI 570 CATCCGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PacI 244 TTAATTAA TTAAT^TAA
PflFI 382 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 316 ACCGGT A^CCGGT
PshAI 273 GACCCTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 406 CAGCTG CAG^CTG
RcaI 500 TCATGA T^CATGA
SacII 95 CCGCGG CCGC^GG
SapI 54 GAAGAGC GCTCTTC(1/4)
SexAI 383 ACCTGGT A^CC[AT]GGT
SgrAI 315 CACCGGTG C[GA]^CCGG[CT]G
StyI 323 CCAAGG C^C[AT][AT]GG
TaqI 551 TCGA T^CGA
TseI 418 GCTGC G^C[AT]GC
TspRI 600 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 382 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 551 TCGA T^CGA
XcmI 374 CCAAACCCGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
Aor51HI 84 AGCGCT AGC^GCT
446 AGCGCT
AvaII 42 GGACC G^G[AT]CC
387 GGTCC
BpmI 397 CTCCAG CTGGAG(16/14)
31 CTGGAG
DdeI 157 CTGAG C^T[ACGT]AG
584 CTGAG
DpnII 134 GATC ^GATC
193 GATC
Eco47III 84 AGCGCT AGC^GCT
446 AGCGCT
MaeII 185 ACGT A^CGT
362 ACGT
MboI 134 GATC ^GATC
193 GATC
MboII 54 GAAGA GAAGA(8/7)
525 GAAGA
MseI 244 TTAA T^TAA
248 TTAA
NdeII 134 GATC ^GATC
193 GATC
NlaIV 122 GGTTCC GG[ACGT]^[ACGT]CC
319 GGTGCC
Sau3AI 134 GATC ^GATC
193 GATC
Tru9I 244 TTAA T^TAA
248 TTAA
Tsp45I 182 GTGAC ^GT[GC]AC
271 GTGAC
Tsp509I 246 AATT ^AATT
331 AATT
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AccII AciI AluI AspHI BcnI
BmyI BsaJI BsaWI BsiHKAI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HpaII
ItaI MaeIII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
Sau96I ScrFI SfaNI