Restriction map for
pFN21AE9412
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AlwNI
AocI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I Asp718I AspI
AvaI AviII AvrII BamHI BanII
BbeI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaWI BseAI BseRI
BsiWI BsmI BsoBI BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstF5I BstPI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EarI Eco47III
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FokI FseI
FspI HincII HindII HindIII HpaI
KasI KpnI Ksp632I MamI MfeI
MflI MluI MroI MseI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspI SwaI TaqI Tru9I Tth111I
TthHB8I Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AhdI 756 GACGATCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 210 GTGCAC G^TGCAC
AlwI 759 GATCC GGATC(4/5)
ApaLI 210 GTGCAC G^TGCAC
AspEI 756 GACGATCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 836 TGGCCA TGG^CCA
BbrPI 700 CACGTG CAC^GTG
BbsI 562 GTCTTC GAAGAC(2/6)
BcnI 497 CCCGG CC^[GC]GG
BfaI 97 CTAG C^TAG
BpuAI 562 GTCTTC GAAGAC(2/6)
BsaAI 700 CACGTG [CT]AC^GT[GA]
BsaI 160 GAGACC GGTCTC(1/5)
BsiEI 401 CGGCCG CG[GA][CT]^CG
BsmBI 754 GAGACG CGTCTC(1/5)
BsmFI 384 GTCCC GGGAC(10/14)
Bsp1407I 773 TGTACA T^GTACA
BspMI 542 ACCTGC ACCTGC(4/8)
BsrGI 773 TGTACA T^GTACA
BsrI 864 ACTGG ACTGG(1/-1)
EagI 401 CGGCCG C^GGCCG
Eam1105I 756 GACGATCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 401 CGGCCG C^GGCCG
Eco52I 401 CGGCCG C^GGCCG
HaeII 120 GGCGCT [GA]GCGC^[CT]
HapII 498 CCGG C^CGG
HpaII 498 CCGG C^CGG
KspI 372 CCGCGG CCGC^GG
MaeI 97 CTAG C^TAG
MboII 563 TCTTC GAAGA(8/7)
MluNI 836 TGGCCA TGG^CCA
MscI 836 TGGCCA TGG^CCA
MspI 498 CCGG C^CGG
NciI 497 CCCGG CC^[GC]GG
NcoI 515 CCATGG C^CATGG
NspI 143 GCATGC [GA]CATG^[CT]
PleI 904 GACTC GAGTC(4/5)
PmaCI 700 CACGTG CAC^GTG
PmlI 700 CACGTG CAC^GTG
SacII 372 CCGCGG CCGC^GG
SphI 143 GCATGC GCATG^C
SspBI 773 TGTACA T^GTACA
StuI 99 AGGCCT AGG^CCT
Tsp509I 505 AATT ^AATT
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AfaI 300 GTAC GT^AC
774 GTAC
AspHI 210 GTGCAC G[AT]GC[AT]^C
492 GAGCAC
BanI 7 GGCACC G^G[CT][GA]CC
356 GGCACC
BpmI 27 CTCCAG CTGGAG(16/14)
751 CTGGAG
BsiHKAI 210 GTGCAC G[AT]GC[AT]^C
492 GAGCAC
BstAPI 231 GCACTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
357 GCACCCGCTGC
DpnII 54 GATC ^GATC
759 GATC
EcoT14I 515 CCATGG C^C[AT][AT]GG
839 CCAAGG
HphI 582 GGTGA GGTGA(8/7)
38 TCACC
MaeII 614 ACGT A^CGT
701 ACGT
MboI 54 GATC ^GATC
759 GATC
NdeII 54 GATC ^GATC
759 GATC
RsaI 300 GTAC GT^AC
774 GTAC
Sau3AI 54 GATC ^GATC
759 GATC
StyI 515 CCATGG C^C[AT][AT]GG
839 CCAAGG
TfiI 465 GATTC G^A[AT]TC
536 GATTC
XcmI 179 CCAACCTGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
800 CCAACTGCTGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AccII AciI AcyI AluI AvaII
BbvI BmyI BsaHI BsaJI BsgI
BsiYI BslI BsmAI Bsp1286I BssKI
BstNI BstUI BstXI Cac8I CfoI
Cfr13I DdeI DsaI EaeI EcoRII
FauI Fnu4HI HaeIII HgaI HhaI
Hin1I HinP1I HinfI ItaI MaeIII
MnlI MslI MspA1I MvaI MvnI
MwoI NlaIII NlaIV Sau96I ScrFI
SfaNI TseI Tsp45I TspRI