Restriction map for
pFN21AE6480
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
AocI Aor51HI AscI AseI AsnI
Asp718I AspEI AspI AvrII BamHI
BbeI BbrPI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaBI BsgI BsiEI
Bsp1407I BspDI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstEII BstPI
BstXI Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV EcoT22I FbaI FseI
HindIII HpaI KasI KpnI MaeI
MamI MfeI MluI MslI MunI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 1065 GACGTC GACGT^C
AccI 3 GTATAC GT^[AC][GT]AC
AccIII 839 TCCGGA T^CCGGA
AcsI 190 GAATTC [GA]^AATT[CT]
AlwNI 701 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 258 GGGCCC GGGCC^C
ApoI 190 GAATTC [GA]^AATT[CT]
AvaI 757 CTCGGG C^[CT]CG[GA]G
AviII 547 TGCGCA TGC^GCA
BcnI 812 CCGGG CC^[GC]GG
BpmI 561 CTGGAG CTGGAG(16/14)
BsaAI 1209 CACGTA [CT]AC^GT[GA]
BsaI 769 GAGACC GGTCTC(1/5)
BseAI 839 TCCGGA T^CCGGA
BsiWI 1211 CGTACG C^GTACG
BsmBI 465 GAGACG CGTCTC(1/5)
BsoBI 757 CTCGGG C^[CT]CG[GA]G
BspEI 839 TCCGGA T^CCGGA
BsrDI 1358 CATTGC GCAATG(2/0)
Bst1107I 3 GTATAC GTA^TAC
BstBI 1481 TTCGAA TT^CGAA
BstYI 1385 GGATCT [GA]^GATC[CT]
BstZ17I 3 GTATAC GTA^TAC
EcoRI 190 GAATTC G^AATTC
EcoT14I 582 CCAAGG C^C[AT][AT]GG
FauI 413 GCGGG CCCGC(4/6)
FspI 547 TGCGCA TGC^GCA
HaeII 618 GGCGCT [GA]GCGC^[CT]
KspI 204 CCGCGG CCGC^GG
MaeIII 1282 GTGAC ^GT[ACGT]AC
MflI 1385 GGATCT [GA]^GATC[CT]
MroI 839 TCCGGA T^CCGGA
MseI 281 TTAA T^TAA
NciI 812 CCGGG CC^[GC]GG
NdeI 1425 CATATG CA^TATG
NspV 1481 TTCGAA TT^CGAA
SacII 204 CCGCGG CCGC^GG
SfuI 1481 TTCGAA TT^CGAA
StyI 582 CCAAGG C^C[AT][AT]GG
Tru9I 281 TTAA T^TAA
Tsp45I 1282 GTGAC ^GT[GC]AC
XhoII 1385 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccII 205 CGCG CG^CG
469 CGCG
AcyI 1065 GACGTC G[GA]^CG[CT]C
1111 GGCGTC
Alw44I 127 GTGCAC G^TGCAC
1520 GTGCAC
ApaLI 127 GTGCAC G^TGCAC
1520 GTGCAC
Asp700I 882 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1146 GAATGTCTTC
AvaII 500 GGACC G^G[AT]CC
1366 GGACC
BalI 1160 TGGCCA TGG^CCA
1313 TGGCCA
BanI 293 GGTGCC G^G[CT][GA]CC
586 GGTGCC
BbvCI 231 CCTCAGC CCTCAGC(-5/-2)
486 CCTCAGC
BsaHI 1065 GACGTC G[GA]^CG[CT]C
1111 GGCGTC
BsaWI 502 ACCGGA [AT]^CCGG[AT]
839 TCCGGA
BseRI 114 CTCCTC GAGGAG(10/8)
766 GAGGAG
BsmAI 465 GAGAC GTCTC(1/5)
769 GAGAC
BsmI 567 GAATGC GAATGC(1/-1)
1478 GCATTC
BspHI 12 TCATGA T^CATGA
623 TCATGA
BspLU11I 827 ACATGT A^CATGT
1524 ACATGT
BstUI 205 CGCG CG^CG
469 CGCG
DsaI 204 CCGCGG C^C[GA][CT]GG
494 CCACGG
EaeI 1160 TGGCCA [CT]^GGCC[GA]
1313 TGGCCA
EcoNI 474 CCTCGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1372 CCTTTTGCAGG
HgaI 467 GACGC GACGC(5/10)
1112 GCGTC
Hin1I 1065 GACGTC G[GA]^CG[CT]C
1111 GGCGTC
HincII 325 GTCAAC GT[CT]^[GA]AC
714 GTCAAC
HindII 325 GTCAAC GT[CT]^[GA]AC
714 GTCAAC
MluNI 1160 TGGCCA TGG^CCA
1313 TGGCCA
MscI 1160 TGGCCA TGG^CCA
1313 TGGCCA
MvnI 205 CGCG CG^CG
469 CGCG
NspI 827 ACATGT [GA]CATG^[CT]
1524 ACATGT
PleI 392 GAGTC GAGTC(4/5)
875 GAGTC
PvuII 398 CAGCTG CAG^CTG
858 CAGCTG
RcaI 12 TCATGA T^CATGA
623 TCATGA
TspRI 62 CACTG CA[GC]TG[ACGT][ACGT]^
1254 CAGTG
XmnI 882 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1146 GAATGTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AspHI BanII BbsI BbvI BmyI
BpuAI BsaJI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MboI MboII
MnlI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI TaqI TfiI
TseI Tsp509I TthHB8I