Restriction map for
pFN21AE6386
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglII BlnI BlpI BmyI
Bpu1102I BsaHI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1286I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstUI BstXI BstYI
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HindIII HpaI HpaII
KasI KpnI Ksp632I KspI MfeI
MflI MluI MluNI MroI MscI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI StyI SwaI Tth111I
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 352 GTATAC GT^[AC][GT]AC
AcsI 382 GAATTT [GA]^AATT[CT]
AflIII 785 ACACGT A^C[GA][CT]GT
AlwI 68 GGATC GGATC(4/5)
AlwNI 840 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 382 GAATTT [GA]^AATT[CT]
AvaI 820 CTCGAG C^[CT]CG[GA]G
AvaII 849 GGACC G^G[AT]CC
BbsI 290 GAAGAC GAAGAC(2/6)
BglI 473 GCCCTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 290 GAAGAC GAAGAC(2/6)
BsaAI 786 CACGTA [CT]AC^GT[GA]
BsaBI 730 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 912 GAGGAG GAGGAG(10/8)
BsgI 650 GTGCAG GTGCAG(16/14)
BsmAI 915 GAGAC GTCTC(1/5)
BsoBI 820 CTCGAG C^[CT]CG[GA]G
Bsp1407I 611 TGTACA T^GTACA
BspMI 356 ACCTGC ACCTGC(4/8)
BsrGI 611 TGTACA T^GTACA
Bst1107I 352 GTATAC GTA^TAC
BstEII 676 GGTTACC G^GT[ACGT]ACC
BstPI 676 GGTTACC G^GT[ACGT]ACC
BstZ17I 352 GTATAC GTA^TAC
DraII 621 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 621 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 676 GGTTACC G^GT[ACGT]ACC
EcoRV 722 GATATC GAT^ATC
HincII 487 GTCAAC GT[CT]^[GA]AC
HindII 487 GTCAAC GT[CT]^[GA]AC
MaeII 787 ACGT A^CGT
MamI 730 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 223 CATGCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 919 CAGCGG C[AC]G^C[GT]G
NlaIV 848 GGGACC GG[ACGT]^[ACGT]CC
NspI 646 GCATGT [GA]CATG^[CT]
PaeR7I 820 CTCGAG C^TCGAG
SfcI 419 CTGTAG C^T[GA][CT]AG
SspBI 611 TGTACA T^GTACA
SspI 76 AATATT AAT^ATT
Tsp45I 88 GTCAC ^GT[GC]AC
XbaI 282 TCTAGA T^CTAGA
XcmI 465 CCATTATTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 820 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 17 enzymes) #
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BbvI 195 GCAGC GCAGC(8/12)
713 GCAGC
BfaI 94 CTAG C^TAG
283 CTAG
BsmFI 565 GGGAC GGGAC(10/14)
848 GGGAC
BstF5I 257 CATCC GGATG(2/0)
390 GGATG
FokI 257 CATCC GGATG(9/13)
390 GGATG
HinfI 594 GATTC G^A[ACGT]TC
932 GATTC
MaeI 94 CTAG C^TAG
283 CTAG
MseI 458 TTAA T^TAA
701 TTAA
PflMI 260 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
839 CCAGCCTCTGG
SfaNI 256 GCATC GCATC(5/9)
582 GCATC
SmlI 617 CTCAAG C^T[CT][GA]AG
820 CTCGAG
TfiI 594 GATTC G^A[AT]TC
932 GATTC
Tru9I 458 TTAA T^TAA
701 TTAA
TseI 195 GCAGC G^C[AT]GC
713 GCAGC
Tsp509I 368 AATT ^AATT
383 AATT
TspRI 143 CACTG CA[GC]TG[ACGT][ACGT]^
669 CAGTG
Van91I 260 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
839 CCAGCCTCTGG
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# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AfaI AluI BpmI BsaJI
BsiYI BslI BsrI BssKI BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI HaeIII HphI ItaI MaeIII
MboI MboII MnlI MvaI MwoI
NdeII NlaIII RsaI Sau3AI Sau96I
ScrFI TaqI TthHB8I