Restriction map for
pFN21AB5921
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AgeI AhdI Alw44I AlwNI
Aor51HI ApaLI AscI AseI AsnI
AspEI AspHI AviII AvrII BalI
BamHI BbeI BbrPI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsiHKAI BsiWI BsmI BspDI
BspEI BspHI BspLU11I BsrBI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
EcoO65I EcoRI EcoT14I EcoT22I FbaI
FseI FspI Hin1I HindIII HpaI
KasI KspI MamI MfeI MluI
MluNI MroI MscI MseI MunI
NarI NcoI NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI StuI StyI SwaI Tru9I
Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AcsI 481 AAATTC [GA]^AATT[CT]
AflIII 1472 ACACGT A^C[GA][CT]GT
AocI 1168 CCTCAGG CC^T[ACGT]AGG
ApoI 481 AAATTC [GA]^AATT[CT]
Asp700I 1470 GAACACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 1380 GGACC G^G[AT]CC
BbsI 1501 GAAGAC GAAGAC(2/6)
BbvCI 587 CCTCAGC CCTCAGC(-5/-2)
BglI 757 GCCCAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1501 GAAGAC GAAGAC(2/6)
BsaI 925 GAGACC GGTCTC(1/5)
BsgI 261 GTGCAG GTGCAG(16/14)
BsiEI 37 CGGCCG CG[GA][CT]^CG
BsmBI 246 CGTCTC CGTCTC(1/5)
Bsp1407I 1014 TGTACA T^GTACA
BsrDI 1154 GCAATG GCAATG(2/0)
BsrFI 13 GCCGGC [GA]^CCGG[CT]
BsrGI 1014 TGTACA T^GTACA
BstXI 270 CCAGGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1168 CCTCAGG CC^T[ACGT]AGG
Cfr10I 13 GCCGGC [GA]^CCGG[CT]
EagI 37 CGGCCG C^GGCCG
EclXI 37 CGGCCG C^GGCCG
Eco52I 37 CGGCCG C^GGCCG
Eco81I 1168 CCTCAGG CC^T[ACGT]AGG
EcoRV 1444 GATATC GAT^ATC
HaeII 16 GGCGCT [GA]GCGC^[CT]
HincII 112 GTCAAC GT[CT]^[GA]AC
HindII 112 GTCAAC GT[CT]^[GA]AC
NaeI 13 GCCGGC GCC^GGC
NdeI 1303 CATATG CA^TATG
NgoMIV 13 GCCGGC G^CCGGC
NspI 380 GCATGT [GA]CATG^[CT]
PstI 1284 CTGCAG CTGCA^G
SapI 1308 GAAGAGC GCTCTTC(1/4)
SspBI 1014 TGTACA T^GTACA
TfiI 1029 GATTC G^A[AT]TC
XmnI 1470 GAACACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Acc65I 266 GGTACC G^GTACC
1203 GGTACC
AlwI 255 GATCC GGATC(4/5)
967 GATCC
ApaI 42 GGGCCC GGGCC^C
1123 GGGCCC
Asp718I 266 GGTACC G^GTACC
1203 GGTACC
AspI 418 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1381 GACCCTGTC
BanII 42 GGGCCC G[GA]GC[CT]^C
1123 GGGCCC
BfaI 743 CTAG C^TAG
1297 CTAG
BseRI 499 GAGGAG GAGGAG(10/8)
502 GAGGAG
BsmFI 1179 GGGAC GGGAC(10/14)
234 GTCCC
BspMI 263 GCAGGT ACCTGC(4/8)
321 GCAGGT
BstYI 254 AGATCC [GA]^GATC[CT]
966 AGATCC
DraII 1122 GGGGCCC [GA]G^G[ACGT]CC[CT]
1123 GGGCCCT
EcoNI 96 CCTCGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1384 CCTGTCACAGG
EcoO109I 1122 GGGGCCC [GA]G^G[ACGT]CC[CT]
1123 GGGCCCT
KpnI 266 GGTACC GGTAC^C
1203 GGTACC
MaeI 743 CTAG C^TAG
1297 CTAG
MaeIII 325 GTGAC ^GT[ACGT]AC
1387 GTCAC
MflI 254 AGATCC [GA]^GATC[CT]
966 AGATCC
MslI 888 CACTGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1266 CACCACGGTG
PflFI 418 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1381 GACCCTGTC
PleI 622 GACTC GAGTC(4/5)
1181 GACTC
SmlI 412 CTCAAG C^T[CT][GA]AG
1435 CTCAAG
Tsp45I 325 GTGAC ^GT[GC]AC
1387 GTCAC
Tth111I 418 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1381 GACCCTGTC
XcmI 839 CCAATCAACTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1441 CCAGATATCGCTTGG
XhoII 254 AGATCC [GA]^GATC[CT]
966 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AfaI AluI AvaI
BanI BbvI BcnI BmyI BpmI
BsaJI BsiYI BslI BsmAI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EaeI EarI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI TaqI
TseI Tsp509I TspRI TthHB8I XmaCI
XmaI