Restriction map for
pFN21AE3151
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI ApaLI ApoI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BlnI BpmI BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI DraIII Eam1105I
EarI Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
Ksp632I MaeI MaeII MamI MfeI
MluI MluNI MroI MscI MseI
MslI MunI NcoI NdeI NheI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SapI SbfI ScaI SfiI SfuI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TaqI TfiI Tru9I
Tsp509I Tth111I TthHB8I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AfaI 717 GTAC GT^AC
AlwNI 919 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
AscI 1024 GGCGCGCC GG^CGCGCC
AspHI 175 GTGCTC G[AT]GC[AT]^C
BamHI 327 GGATCC G^GATCC
BglII 827 AGATCT A^GATCT
BsgI 535 GTGCAG GTGCAG(16/14)
BsiHKAI 175 GTGCTC G[AT]GC[AT]^C
BsmAI 15 GTCTC GTCTC(1/5)
BssHII 1025 GCGCGC G^CGCGC
BssSI 853 CTCGTG CACGAG(-5/-1)
BstAPI 918 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 353 CCAAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 277 GACCCCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 38 CCAAGG C^C[AT][AT]GG
MaeIII 247 GTGAC ^GT[ACGT]AC
NaeI 258 GCCGGC GCC^GGC
NgoMIV 258 GCCGGC G^CCGGC
NlaIII 816 CATG CATG^
NotI 427 GCGGCCGC GC^GGCCGC
PflMI 1073 CCAAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 1056 CAGCTG CAG^CTG
RsaI 717 GTAC GT^AC
SexAI 704 ACCTGGT A^CC[AT]GGT
SmaI 615 CCCGGG CCC^GGG
StuI 683 AGGCCT AGG^CCT
StyI 38 CCAAGG C^C[AT][AT]GG
Tsp45I 247 GTGAC ^GT[GC]AC
Van91I 1073 CCAAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 615 CCCGGG C^CCGGG
XmaI 615 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwI 328 GATCC GGATC(4/5)
327 GGATC
Aor51HI 872 AGCGCT AGC^GCT
884 AGCGCT
ApaI 270 GGGCCC GGGCC^C
1008 GGGCCC
BstF5I 263 CATCC GGATG(2/0)
900 CATCC
BstYI 327 GGATCC [GA]^GATC[CT]
827 AGATCT
DpnII 328 GATC ^GATC
828 GATC
DraII 270 GGGCCCC [GA]G^G[ACGT]CC[CT]
1007 GGGGCCC
Eco47III 872 AGCGCT AGC^GCT
884 AGCGCT
EcoO109I 270 GGGCCCC [GA]G^G[ACGT]CC[CT]
1007 GGGGCCC
FokI 263 CATCC GGATG(9/13)
900 CATCC
HinfI 158 GACTC G^A[ACGT]TC
733 GAGTC
MboI 328 GATC ^GATC
828 GATC
MflI 327 GGATCC [GA]^GATC[CT]
827 AGATCT
NdeII 328 GATC ^GATC
828 GATC
PleI 158 GACTC GAGTC(4/5)
733 GAGTC
PstI 664 CTGCAG CTGCA^G
772 CTGCAG
Sau3AI 328 GATC ^GATC
828 GATC
SfaNI 921 GATGC GCATC(5/9)
262 GCATC
SfcI 664 CTGCAG C^T[GA][CT]AG
772 CTGCAG
SgrAI 500 CGCCGGTG C[GA]^CCGG[CT]G
546 CACCGGCG
XhoII 327 GGATCC [GA]^GATC[CT]
827 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AcyI AluI AvaI
AvaII BanI BanII BbeI BbvI
BcnI BglI BlpI BmyI Bpu1102I
BsaHI BsaJI BseRI BsiEI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstNI
BstUI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DsaI EaeI EagI
EclXI Eco52I EcoRII FauI Fnu4HI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII HphI ItaI
KasI KspI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NlaIV SacII Sau96I ScrFI
SfoI SmlI TseI TspRI