Restriction map for
pFN21AE2651
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# NO CUT ENZYMES ( 89 enzymes) #
##################################
AclI AcsI AflII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI AspEI AviII BcgI
BciVI BclI BfrI BglI BsaBI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrDI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DrdI Eam1105I
Eco47III EcoO65I EcoRI EcoRV FbaI
FseI FspI HincII HindII HpaI
KspI MamI MfeI MluI MseI
MunI NcoI NdeI NheI NotI
NruI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I XbaI XhoI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AatII 403 GACGTC GACGT^C
Acc65I 1230 GGTACC G^GTACC
AccI 1492 GTCTAC GT^[AC][GT]AC
AccIII 350 TCCGGA T^CCGGA
ApaI 1534 GGGCCC GGGCC^C
Asp700I 471 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1230 GGTACC G^GTACC
AspHI 2335 GAGCTC G[AT]GC[AT]^C
AvrII 1071 CCTAGG C^CTAGG
BamHI 2142 GGATCC G^GATCC
BbeI 1570 GGCGCC GGCGC^C
BbsI 1972 GTCTTC GAAGAC(2/6)
BbvCI 2227 GCTGAGG CCTCAGC(-5/-2)
BglII 881 AGATCT A^GATCT
BlnI 1071 CCTAGG C^CTAGG
BlpI 9 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 9 GCTGAGC GC^T[ACGT]AGC
BpuAI 1972 GTCTTC GAAGAC(2/6)
BseAI 350 TCCGGA T^CCGGA
BsiHKAI 2335 GAGCTC G[AT]GC[AT]^C
BspEI 350 TCCGGA T^CCGGA
BsrFI 738 GCCGGC [GA]^CCGG[CT]
CelII 9 GCTGAGC GC^T[ACGT]AGC
Cfr10I 738 GCCGGC [GA]^CCGG[CT]
DraIII 1084 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1097 CCGTGG C^C[GA][CT]GG
EcoNI 861 CCTGGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1138 ATGCAT ATGCA^T
KasI 1570 GGCGCC G^GCGCC
KpnI 1230 GGTACC GGTAC^C
MroI 350 TCCGGA T^CCGGA
NaeI 738 GCCGGC GCC^GGC
NarI 1570 GGCGCC GG^CGCC
NgoMIV 738 GCCGGC G^CCGGC
NsiI 1138 ATGCAT ATGCA^T
PstI 55 CTGCAG CTGCA^G
SacI 2335 GAGCTC GAGCT^C
SapI 1032 GCTCTTC GCTCTTC(1/4)
SfoI 1570 GGCGCC GGC^GCC
SmaI 48 CCCGGG CCC^GGG
XmaCI 48 CCCGGG C^CCGGG
XmaI 48 CCCGGG C^CCGGG
XmnI 471 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 289 CGCG CG^CG
1604 CGCG
AcyI 403 GACGTC G[GA]^CG[CT]C
1570 GGCGCC
AflIII 887 ACGTGT A^C[GA][CT]GT
2252 ACGTGT
AlwNI 409 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
829 CAGGACCTG
AocI 1064 CCTCAGG CC^T[ACGT]AGG
1150 CCTCAGG
AvaI 48 CCCGGG C^[CT]CG[GA]G
2341 CCCGAG
BbrPI 382 CACGTG CAC^GTG
682 CACGTG
BsaHI 403 GACGTC G[GA]^CG[CT]C
1570 GGCGCC
BsiEI 713 CGGCCG CG[GA][CT]^CG
1016 CGGCCG
BsoBI 48 CCCGGG C^[CT]CG[GA]G
2341 CCCGAG
BsrBI 601 GAGCGG CCGCTC(-3/-3)
1696 GAGCGG
BssSI 844 CACGAG CACGAG(-5/-1)
2104 CACGAG
BstUI 289 CGCG CG^CG
1604 CGCG
Bsu36I 1064 CCTCAGG CC^T[ACGT]AGG
1150 CCTCAGG
EagI 713 CGGCCG C^GGCCG
1016 CGGCCG
EclXI 713 CGGCCG C^GGCCG
1016 CGGCCG
Eco52I 713 CGGCCG C^GGCCG
1016 CGGCCG
Eco81I 1064 CCTCAGG CC^T[ACGT]AGG
1150 CCTCAGG
HgaI 1481 GACGC GACGC(5/10)
1051 GCGTC
Hin1I 403 GACGTC G[GA]^CG[CT]C
1570 GGCGCC
HindIII 114 AAGCTT A^AGCTT
514 AAGCTT
MvnI 289 CGCG CG^CG
1604 CGCG
PmaCI 382 CACGTG CAC^GTG
682 CACGTG
PmlI 382 CACGTG CAC^GTG
682 CACGTG
PvuII 230 CAGCTG CAG^CTG
2417 CAGCTG
SexAI 1841 ACCTGGT A^CC[AT]GGT
1967 ACCAGGT
TfiI 789 GAATC G^A[AT]TC
1240 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AfaI AluI AlwI AspI
AvaII BalI BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaAI
BsaI BsaJI BsaWI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII