Restriction map for
pFN21AB5790
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# NO CUT ENZYMES ( 120 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AgeI AhdI Alw44I AocI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII BbeI
BbrPI BcgI BglI BsaAI BsaHI
BseAI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BsrGI BssHII
BssSI Bst1107I BstEII BstPI BstXI
BstZ17I Bsu36I ClaI CpoI DraI
DraII DraIII EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT22I FauI FseI
FspI Hin1I HincII HindII HpaI
KasI KpnI Ksp632I KspI MfeI
MluI MroI MunI NaeI NarI
NgoMIV NotI NruI NsiI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 1916 GTCTAC GT^[AC][GT]AC
AflII 1774 CTTAAG C^TTAAG
AflIII 1145 ACATGT A^C[GA][CT]GT
AlwNI 800 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 2189 AGCGCT AGC^GCT
ApaI 101 GGGCCC GGGCC^C
AvrII 382 CCTAGG C^CTAGG
BalI 966 TGGCCA TGG^CCA
BamHI 38 GGATCC G^GATCC
BanII 101 GGGCCC G[GA]GC[CT]^C
BbsI 1731 GTCTTC GAAGAC(2/6)
BclI 2184 TGATCA T^GATCA
BfrI 1774 CTTAAG C^TTAAG
BglII 601 AGATCT A^GATCT
BlnI 382 CCTAGG C^CTAGG
BpuAI 1731 GTCTTC GAAGAC(2/6)
BsaBI 2179 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2172 GAGACC GGTCTC(1/5)
BsaWI 81 ACCGGA [AT]^CCGG[AT]
BseRI 1280 GAGGAG GAGGAG(10/8)
BsgI 2196 CTGCAC GTGCAG(16/14)
BsiEI 123 CGACCG CG[GA][CT]^CG
BsmI 1016 GCATTC GAATGC(1/-1)
BspLU11I 1145 ACATGT A^CATGT
BspMI 2089 GCAGGT ACCTGC(4/8)
BsrBI 42 CCGCTC CCGCTC(-3/-3)
BsrFI 26 ACCGGC [GA]^CCGG[CT]
BstBI 332 TTCGAA TT^CGAA
Cfr10I 26 ACCGGC [GA]^CCGG[CT]
DrdI 980 GACTTTCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 74 CCATGG C^C[GA][CT]GG
EaeI 966 TGGCCA [CT]^GGCC[GA]
Eco47III 2189 AGCGCT AGC^GCT
FbaI 2184 TGATCA T^GATCA
HaeII 2189 AGCGCT [GA]GCGC^[CT]
HgaI 67 GCGTC GACGC(5/10)
MamI 2179 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 966 TGGCCA TGG^CCA
MscI 966 TGGCCA TGG^CCA
MspA1I 1996 CAGCTG C[AC]G^C[GT]G
NcoI 74 CCATGG C^CATGG
NdeI 536 CATATG CA^TATG
NheI 1660 GCTAGC G^CTAGC
NspV 332 TTCGAA TT^CGAA
PvuII 1996 CAGCTG CAG^CTG
SexAI 1173 ACCAGGT A^CC[AT]GGT
SfuI 332 TTCGAA TT^CGAA
SmlI 1774 CTTAAG C^T[CT][GA]AG
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# TWO CUT ENZYMES ( 13 enzymes) #
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AvaII 1702 GGACC G^G[AT]CC
1966 GGTCC
BbvCI 1232 CCTCAGC CCTCAGC(-5/-2)
1655 CCTCAGC
BciVI 272 GGATAC GTATCC(6/5)
718 GTATCC
BlpI 523 GCTCAGC GC^T[ACGT]AGC
847 GCTGAGC
Bpu1102I 523 GCTCAGC GC^T[ACGT]AGC
847 GCTGAGC
BsmFI 989 GTCCC GGGAC(10/14)
1229 GTCCC
BstAPI 1047 GCAAACGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2161 GCATACCATGC
CelII 523 GCTCAGC GC^T[ACGT]AGC
847 GCTGAGC
EcoRI 420 GAATTC G^AATTC
1156 GAATTC
EcoT14I 74 CCATGG C^C[AT][AT]GG
382 CCTAGG
MslI 346 CATGACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1094 CACCTTCATG
PstI 563 CTGCAG CTGCA^G
2087 CTGCAG
StyI 74 CCATGG C^C[AT][AT]GG
382 CCTAGG
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# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI BanI BbvI
BcnI BfaI BmyI BpmI BsaJI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HindIII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII