Restriction map for
pFN21AE6528
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# NO CUT ENZYMES ( 177 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BanI BbeI BbrPI
BbsI BcgI BciVI BclI BfaI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
Cac8I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeI MaeII MaeIII MamI
MfeI MluI MluNI MroI MscI
MseI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
TfiI Tru9I Tsp45I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AcsI 362 AAATTC [GA]^AATT[CT]
ApoI 362 AAATTC [GA]^AATT[CT]
AspHI 313 GAGCTC G[AT]GC[AT]^C
AvaII 342 GGACC G^G[AT]CC
BamHI 395 GGATCC G^GATCC
BcnI 445 CCGGG CC^[GC]GG
BglII 275 AGATCT A^GATCT
BpmI 382 CTGGAG CTGGAG(16/14)
BsiHKAI 313 GAGCTC G[AT]GC[AT]^C
BsmAI 329 GTCTC GTCTC(1/5)
BsmFI 300 GGGAC GGGAC(10/14)
BsmI 52 GAATGC GAATGC(1/-1)
BsrDI 201 GCAATG GCAATG(2/0)
BsrI 162 CCAGT ACTGG(1/-1)
BstAPI 429 GCAGTATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 104 CCATCAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 28 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 28 AGGGCCT [GA]G^G[ACGT]CC[CT]
FauI 249 CCCGC CCCGC(4/6)
HapII 445 CCGG C^CGG
HpaII 445 CCGG C^CGG
HphI 11 TCACC GGTGA(8/7)
MboII 273 GAAGA GAAGA(8/7)
MslI 105 CATCAGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 445 CCGG C^CGG
NciI 445 CCGGG CC^[GC]GG
NlaIII 513 CATG CATG^
PvuII 390 CAGCTG CAG^CTG
SacI 313 GAGCTC GAGCT^C
ScaI 164 AGTACT AGT^ACT
SfaNI 408 GATGC GCATC(5/9)
SfcI 417 CTACAG C^T[GA][CT]AG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwI 396 GATCC GGATC(4/5)
395 GGATC
AlwNI 90 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
430 CAGTATCTG
BanII 313 GAGCTC G[GA]GC[CT]^C
476 GGGCTC
BbvCI 213 CCTCAGC CCTCAGC(-5/-2)
438 GCTGAGG
BmyI 313 GAGCTC G[AGT]GC[ACT]^C
476 GGGCTC
BsiYI 250 CCGCACTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
413 CCAACTACAGG
BslI 250 CCGCACTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
413 CCAACTACAGG
Bsp1286I 313 GAGCTC G[AGT]GC[ACT]^C
476 GGGCTC
BsrBI 228 CCGCTC CCGCTC(-3/-3)
117 GAGCGG
BstNI 36 CCAGG CC^[AT]GG
153 CCAGG
BstYI 275 AGATCT [GA]^GATC[CT]
395 GGATCC
CfoI 141 GCGC GCG^C
260 GCGC
EcoRII 36 CCAGG ^CC[AT]GG
153 CCAGG
HaeII 140 AGCGCC [GA]GCGC^[CT]
259 GGCGCT
HhaI 141 GCGC GCG^C
260 GCGC
HinP1I 141 GCGC G^CGC
260 GCGC
HinfI 220 GACTC G^A[ACGT]TC
509 GAGTC
MflI 275 AGATCT [GA]^GATC[CT]
395 GGATCC
MspA1I 93 CCGCTG C[AC]G^C[GT]G
390 CAGCTG
MvaI 36 CCAGG CC^[AT]GG
153 CCAGG
NlaIV 395 GGATCC GG[ACGT]^[ACGT]CC
477 GGCTCC
PleI 220 GACTC GAGTC(4/5)
509 GAGTC
PshAI 302 GACAAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
322 GACGACTGTC
Tsp509I 293 AATT ^AATT
363 AATT
TspRI 60 CACTG CA[GC]TG[ACGT][ACGT]^
357 CACTG
XhoII 275 AGATCT [GA]^GATC[CT]
395 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AciI AfaI AluI BbvI BsaJI
BssKI BstF5I Cfr13I DdeI DpnII
Fnu4HI FokI HaeIII ItaI MboI
MnlI MwoI NdeII RsaI Sau3AI
Sau96I ScrFI TseI