Restriction map for
pFN21AE2068
##################################
# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BclI BfrI BglI BglII BlnI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
BstEII BstPI BstXI CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HpaI KasI
KpnI KspI MamI MluI MroI
NaeI NarI NcoI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SmlI SnaBI Sse8387I SspBI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 527 GTATAC GT^[AC][GT]AC
AccII 973 CGCG CG^CG
AciI 766 GCGG CCGC(-3/-1)
AcsI 1349 AAATTC [GA]^AATT[CT]
AocI 256 CCTAAGG CC^T[ACGT]AGG
ApoI 1349 AAATTC [GA]^AATT[CT]
Asp700I 1603 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 2120 TGGCCA TGG^CCA
BbvCI 2059 CCTCAGC CCTCAGC(-5/-2)
BcgI 174 GCAGATCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 8 GGATAC GTATCC(6/5)
BlpI 1807 GCTCAGC GC^T[ACGT]AGC
BpmI 800 CTGGAG CTGGAG(16/14)
Bpu1102I 1807 GCTCAGC GC^T[ACGT]AGC
BsaAI 824 CACGTA [CT]AC^GT[GA]
BsgI 1436 CTGCAC GTGCAG(16/14)
BsiEI 140 CGACCG CG[GA][CT]^CG
BspMI 1024 GCAGGT ACCTGC(4/8)
Bst1107I 527 GTATAC GTA^TAC
BstUI 973 CGCG CG^CG
BstZ17I 527 GTATAC GTA^TAC
Bsu36I 256 CCTAAGG CC^T[ACGT]AGG
CelII 1807 GCTCAGC GC^T[ACGT]AGC
DraII 1391 AGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 2120 TGGCCA [CT]^GGCC[GA]
Eco81I 256 CCTAAGG CC^T[ACGT]AGG
EcoO109I 1391 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 670 GATATC GAT^ATC
EcoT22I 829 ATGCAT ATGCA^T
HincII 1789 GTCAAC GT[CT]^[GA]AC
HindII 1789 GTCAAC GT[CT]^[GA]AC
HindIII 152 AAGCTT A^AGCTT
MluNI 2120 TGGCCA TGG^CCA
MscI 2120 TGGCCA TGG^CCA
MspA1I 1472 CAGCTG C[AC]G^C[GT]G
MvnI 973 CGCG CG^CG
NsiI 829 ATGCAT ATGCA^T
NspI 170 GCATGC [GA]CATG^[CT]
PpuMI 1391 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 1472 CAGCTG CAG^CTG
SpeI 1507 ACTAGT A^CTAGT
SphI 170 GCATGC GCATG^C
SspI 778 AATATT AAT^ATT
XmnI 1603 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 536 GTAC GT^AC
1211 GTAC
AlwNI 1124 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1754 CAGGAGCTG
BcnI 133 CCGGG CC^[GC]GG
719 CCCGG
BspHI 195 TCATGA T^CATGA
515 TCATGA
BsrDI 1362 CATTGC GCAATG(2/0)
1485 CATTGC
BstBI 62 TTCGAA TT^CGAA
1253 TTCGAA
BstYI 176 AGATCC [GA]^GATC[CT]
854 GGATCT
HapII 133 CCGG C^CGG
720 CCGG
HpaII 133 CCGG C^CGG
720 CCGG
MfeI 1019 CAATTG C^AATTG
1169 CAATTG
MflI 176 AGATCC [GA]^GATC[CT]
854 GGATCT
MspI 133 CCGG C^CGG
720 CCGG
MunI 1019 CAATTG C^AATTG
1169 CAATTG
NciI 133 CCGGG CC^[GC]GG
719 CCCGG
NspV 62 TTCGAA TT^CGAA
1253 TTCGAA
RcaI 195 TCATGA T^CATGA
515 TCATGA
RsaI 536 GTAC GT^AC
1211 GTAC
SacI 1805 GAGCTC GAGCT^C
2008 GAGCTC
SfuI 62 TTCGAA TT^CGAA
1253 TTCGAA
StuI 23 AGGCCT AGG^CCT
33 AGGCCT
XcmI 749 CCATTGATGTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1443 CCAAAGAAATGATGG
XhoII 176 AGATCC [GA]^GATC[CT]
854 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AluI AlwI AspHI AvaII BanI
BanII BbsI BbvI BfaI BmyI
BpuAI BsaJI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstAPI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MvaI
MwoI NdeI NdeII NlaIII NlaIV
PleI PstI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I