Restriction map for
pFN21AE1960
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# NO CUT ENZYMES ( 147 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BanII BbeI BbrPI BbvCI
BclI BcnI BfrI BglI BglII
BlnI BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HpaI HpaII KasI
KpnI KspI MaeII MamI MfeI
MluI MroI MspI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SspI SwaI Tth111I XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AciI 682 CCGC CCGC(-3/-1)
Alw44I 740 GTGCAC G^TGCAC
AlwNI 1378 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 740 GTGCAC G^TGCAC
AspHI 740 GTGCAC G[AT]GC[AT]^C
BalI 1319 TGGCCA TGG^CCA
BanI 1263 GGCACC G^G[CT][GA]CC
BcgI 1236 CGAAGCAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1339 GTATCC GTATCC(6/5)
BlpI 167 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 167 GCTCAGC GC^T[ACGT]AGC
BsaI 753 GGTCTC GGTCTC(1/5)
BseRI 244 CTCCTC GAGGAG(10/8)
BsiHKAI 740 GTGCAC G[AT]GC[AT]^C
Bsp1407I 824 TGTACA T^GTACA
BsrGI 824 TGTACA T^GTACA
BstXI 942 CCAGTGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 167 GCTCAGC GC^T[ACGT]AGC
DsaI 1071 CCATGG C^C[GA][CT]GG
EaeI 1319 TGGCCA [CT]^GGCC[GA]
HindIII 67 AAGCTT A^AGCTT
HphI 1207 GGTGA GGTGA(8/7)
MluNI 1319 TGGCCA TGG^CCA
MscI 1319 TGGCCA TGG^CCA
NcoI 1071 CCATGG C^CATGG
PflMI 99 CCAAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 512 AGGACCT [GA]G^G[AT]CC[CT]
SbfI 1275 CCTGCAGG CCTGCA^GG
SphI 705 GCATGC GCATG^C
Sse8387I 1275 CCTGCAGG CCTGCA^GG
SspBI 824 TGTACA T^GTACA
Tsp509I 1403 AATT ^AATT
Van91I 99 CCAAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 465 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccI 601 GTAGAC GT^[AC][GT]AC
1090 GTCTAC
AlwI 924 GATCC GGATC(4/5)
204 GGATC
BbsI 220 GAAGAC GAAGAC(2/6)
1420 GAAGAC
BpuAI 220 GAAGAC GAAGAC(2/6)
1420 GAAGAC
BspMI 350 ACCTGC ACCTGC(4/8)
709 GCAGGT
BstYI 204 GGATCT [GA]^GATC[CT]
923 AGATCC
DraII 512 AGGACCT [GA]G^G[ACGT]CC[CT]
1106 AGGCCCT
EcoO109I 512 AGGACCT [GA]G^G[ACGT]CC[CT]
1106 AGGCCCT
EcoT14I 1071 CCATGG C^C[AT][AT]GG
1203 CCAAGG
MflI 204 GGATCT [GA]^GATC[CT]
923 AGATCC
MseI 391 TTAA T^TAA
659 TTAA
MslI 910 CATAAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
970 CACAATAATG
MspA1I 818 CAGCTG C[AC]G^C[GT]G
1365 CAGCTG
PvuII 818 CAGCTG CAG^CTG
1365 CAGCTG
SmlI 8 CTCAAG C^T[CT][GA]AG
115 CTCAAG
SpeI 639 ACTAGT A^CTAGT
1530 ACTAGT
StuI 849 AGGCCT AGG^CCT
1196 AGGCCT
StyI 1071 CCATGG C^C[AT][AT]GG
1203 CCAAGG
TaqI 1235 TCGA T^CGA
1464 TCGA
TfiI 82 GATTC G^A[AT]TC
834 GAATC
Tru9I 391 TTAA T^TAA
659 TTAA
Tsp45I 589 GTGAC ^GT[GC]AC
979 GTGAC
TthHB8I 1235 TCGA T^CGA
1464 TCGA
XhoII 204 GGATCT [GA]^GATC[CT]
923 AGATCC
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AfaI AluI AvaII BbvI BfaI
BmyI BpmI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HaeIII HincII HindII HinfI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TseI TspRI XcmI